scholarly journals Supported lipid bilayers, tethered lipid vesicles, and vesicle fusion investigated using gravimetric, plasmonic, and microscopy techniques

2008 ◽  
Vol 3 (2) ◽  
pp. FA108-FA116 ◽  
Author(s):  
Fredrik Höök ◽  
Gudrun Stengel ◽  
Andreas B. Dahlin ◽  
Anders Gunnarsson ◽  
Magnus P. Jonsson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers ◽  
Microscopy Techniques

scholarly journals The role of surface charge in the interaction of nanoparticles with model pulmonary surfactants

Soft Matter ◽  
2018 ◽  
Vol 14 (28) ◽  
pp. 5764-5774 ◽  
Author(s):  
F. Mousseau ◽  
J.-F. Berret
Keyword(s):  
Surface Charge ◽  
Lipid Bilayers ◽  
Pulmonary Surfactant ◽  
Lipid Vesicles ◽  
Electrostatic Charge ◽  
Supported Lipid Bilayers ◽  
Pulmonary Surfactants ◽  
Inhaled Nanoparticles

Inhaled nanoparticles reaching the respiratory zone in the lungs enter first in contact with the pulmonary surfactant. It is shown here that nanoparticles and lipid vesicles formulated from different surfactant mimetics interact predominantlyviaelectrostatic charge mediated attraction and do not form supported lipid bilayers spontaneously.

scholarly journals Correlative microscopy reveals the nanoscale morphology of E. coli-derived supported lipid bilayers

2021 ◽  
Author(s):  
Karan Bali ◽  
Zeinab Mohamed ◽  
Anna-Maria Pappa ◽  
Susan Daniel ◽  
Clemens F. Kaminski ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membrane ◽  
Lipid Vesicles ◽  
Outer Membrane Vesicles ◽  
Atomic Scale ◽  
Membrane Properties ◽  
Supported Lipid Bilayers ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Natural And Synthetic

Supported lipid bilayers (SLBs) made from reconstituted lipid vesicles are an important tool in molecular biology. A breakthrough in the field has come with the use of vesicles derived from cell membranes to form SLBs. These new supported bilayers, consisting both of natural and synthetic components, provide a physiologically relevant system on which to study protein-protein interactions as well as protein-ligand interactions and other lipid membrane properties. These complex bilayer systems hold promise but have not yet been fully characterised in terms of their composition, ratio of natural to synthetic component and membrane protein content. Here, we describe a method of correlative atomic force (AFM) with structured illumination microscopy (SIM) for the accurate mapping of complex lipid bilayers that consist of a synthetic fraction and a fraction of lipids derived from Escherichia coli outer membrane vesicles (OMVs). We exploit the enhanced resolution and molecular specificity that SIM can offer to identify areas of interest in these bilayers and the atomic scale resolution that the AFM provides to create detailed topography maps of the bilayers. We are thus able to understand the way in which the two different lipid fractions (natural and synthetic) mix within the bilayers, quantify the amount of bacterial membrane incorporated in the bilayer and directly visualise the interaction of these bilayers with bacteria-specific, membrane-binding proteins. Our work sets the foundation for accurately understanding the composition and properties of OMV-derived SLBs and establishes correlative AFM/ SIM as a method for characterising complex systems at the nanoscale.

scholarly journals Nanoparticle-Lipid Interaction: Job Scattering Plots to Differentiate Vesicle Aggregation from Supported Lipid Bilayer Formation

2018 ◽  
Vol 2 (4) ◽  
pp. 50 ◽  
Author(s):  
Fanny Mousseau ◽  
Evdokia Oikonomou ◽  
Victor Baldim ◽  
Stéphane Mornet ◽  
Jean-François Berret
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Living Cells ◽  
Supported Lipid Bilayers ◽  
Supported Lipid Bilayer ◽  
Mixed Aggregates ◽  
Method Of Continuous Variation ◽  
The Impact ◽  
Analytical Expressions ◽  
Lipid Interaction

The impact of nanomaterials on lung fluids, or on the plasma membrane of living cells, has prompted researchers to examine the interactions between nanoparticles and lipid vesicles. Recent studies have shown that nanoparticle-lipid interaction leads to a broad range of structures including supported lipid bilayers (SLB), particles adsorbed at the surface or internalized inside vesicles, and mixed aggregates. Currently, there is a need to have simple protocols that can readily evaluate the structures made from particles and vesicles. Here we apply the method of continuous variation for measuring Job scattering plots and provide analytical expressions for the scattering intensity in various scenarios. The result that emerges from the comparison between experiments and modeling is that electrostatics play a key role in the association, but it is not sufficient to induce the formation of supported lipid bilayers.

scholarly journals Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature

Langmuir ◽  
2014 ◽  
Vol 30 (25) ◽  
pp. 7259-7263 ◽  
Author(s):  
Tania Kjellerup Lind ◽  
Marité Cárdenas ◽  
Hanna Pauliina Wacklin
Keyword(s):  
Lipid Bilayers ◽  
Deposition Temperature ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers

scholarly journals Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers

2010 ◽  
Vol 98 (3) ◽  
pp. 673a
Author(s):  
Lisa V. Simonsson ◽  
Peter Jönsson ◽  
Gudrun Stengel ◽  
Fredrik Höök
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Supported Lipid Bilayers ◽  
Site Specific ◽  
Controlled Fusion

AFM Studies of Solid-Supported Lipid Bilayers Formed at a Au(111) Electrode Surface Using Vesicle Fusion and a Combination of Langmuir−Blodgett and Langmuir−Schaefer Techniques

Langmuir ◽  
2008 ◽  
Vol 24 (18) ◽  
pp. 10313-10323 ◽  
Author(s):  
Ming Li ◽  
Maohui Chen ◽  
Erin Sheepwash ◽  
Christa L. Brosseau ◽  
Hongqiang Li ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Electrode Surface ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers ◽  
Langmuir Blodgett

scholarly journals Nanoparticle-Lipid Interaction: Job Scattering Plots to Differentiate Vesicle Aggregationfrom Supported Lipid Bilayer Formation

2018 ◽  
Author(s):  
Fanny Mousseau ◽  
Evdokia Oikonomou ◽  
Victor Baldim ◽  
Stéphane Mornet ◽  
Jean-François Berret
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Living Cells ◽  
Supported Lipid Bilayers ◽  
Supported Lipid Bilayer ◽  
Mixed Aggregates ◽  
Method Of Continuous Variation ◽  
The Impact ◽  
Analytical Expressions ◽  
Lipid Interaction

The impact of nanomaterials on lung fluids or on the plasma membrane of living cells has prompted researchers to examine the interactions between nanoparticles and lipid vesicles. Recent studies have shown that nanoparticle-lipid interaction leads to a broad range of structures including supported lipid bilayers (SLB), particles adsorbed at the surface or internalized inside vesicles, and mixed aggregates. Today, there is a need to have simple protocols that can readily assess the nature of structures obtained from particles and vesicles. Here we apply the method of continuous variation for measuring Job scattering plots and provide analytical expressions for the scattering intensity in various scenarios. The result that emerges from the comparison between modeling and experimental measurements is that electrostatics plays a key role in the association, but it is not sufficient to induce the formation of supported lipid bilayers.

scholarly journals Biomimetic supported lipid bilayers with high cholesterol content formed by α-helical peptide-induced vesicle fusion

2012 ◽  
Vol 22 (37) ◽  
pp. 19506 ◽  
Author(s):  
Gregory J. Hardy ◽  
Rahul Nayak ◽  
S. Munir Alam ◽  
Joseph G. Shapter ◽  
Frank Heinrich ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Cholesterol Content ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers ◽  
High Cholesterol ◽  
Helical Peptide
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