Spatial-Resolution Limits in Mass Spectrometry Imaging of Supported Lipid Bilayers and Individual Lipid Vesicles

2010 ◽  
Vol 82 (6) ◽  
pp. 2426-2433 ◽  
Author(s):  
Anders Gunnarsson ◽  
Felix Kollmer ◽  
Sascha Sohn ◽  
Fredrik Höök ◽  
Peter Sjövall
Keyword(s):  
Mass Spectrometry ◽  
Lipid Bilayers ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Lipid Vesicles ◽  
Supported Lipid Bilayers ◽  
Resolution Limits

scholarly journals The role of surface charge in the interaction of nanoparticles with model pulmonary surfactants

Soft Matter ◽  
2018 ◽  
Vol 14 (28) ◽  
pp. 5764-5774 ◽  
Author(s):  
F. Mousseau ◽  
J.-F. Berret
Keyword(s):  
Surface Charge ◽  
Lipid Bilayers ◽  
Pulmonary Surfactant ◽  
Lipid Vesicles ◽  
Electrostatic Charge ◽  
Supported Lipid Bilayers ◽  
Pulmonary Surfactants ◽  
Inhaled Nanoparticles

Inhaled nanoparticles reaching the respiratory zone in the lungs enter first in contact with the pulmonary surfactant. It is shown here that nanoparticles and lipid vesicles formulated from different surfactant mimetics interact predominantlyviaelectrostatic charge mediated attraction and do not form supported lipid bilayers spontaneously.

Modality Agnostic Model for Spatial Resolution in Mass Spectrometry Imaging: Application to MALDI MSI Data

2021 ◽  
Author(s):  
Martin D. Metodiev ◽  
Rory T. Steven ◽  
Xavier Loizeau ◽  
Zoltan Takats ◽  
Josephine Bunch
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Imaging Application

scholarly journals NanoSIMS imaging: an approach for visualizing and quantifying lipids in cells and tissues

2016 ◽  
Vol 65 (3) ◽  
pp. 669-672 ◽  
Author(s):  
Cuiwen He ◽  
Loren G Fong ◽  
Stephen G Young ◽  
Haibo Jiang
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Secondary Ion Mass Spectrometry ◽  
High Spatial Resolution ◽  
High Sensitivity ◽  
New Approach ◽  
The Past ◽  
Secondary Ion ◽  
In Cells

Over the past few decades, several approaches have been used to image lipids in cells and tissues, but most have limited spatial resolution and sensitivity. Here, we discuss a relatively new approach, nanoscale secondary ion mass spectrometry imaging, that makes it possible to visualize lipids in cells and tissues in a quantitative fashion and with high spatial resolution and high sensitivity.

scholarly journals Correlative microscopy reveals the nanoscale morphology of E. coli-derived supported lipid bilayers

2021 ◽  
Author(s):  
Karan Bali ◽  
Zeinab Mohamed ◽  
Anna-Maria Pappa ◽  
Susan Daniel ◽  
Clemens F. Kaminski ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membrane ◽  
Lipid Vesicles ◽  
Outer Membrane Vesicles ◽  
Atomic Scale ◽  
Membrane Properties ◽  
Supported Lipid Bilayers ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Natural And Synthetic

Supported lipid bilayers (SLBs) made from reconstituted lipid vesicles are an important tool in molecular biology. A breakthrough in the field has come with the use of vesicles derived from cell membranes to form SLBs. These new supported bilayers, consisting both of natural and synthetic components, provide a physiologically relevant system on which to study protein-protein interactions as well as protein-ligand interactions and other lipid membrane properties. These complex bilayer systems hold promise but have not yet been fully characterised in terms of their composition, ratio of natural to synthetic component and membrane protein content. Here, we describe a method of correlative atomic force (AFM) with structured illumination microscopy (SIM) for the accurate mapping of complex lipid bilayers that consist of a synthetic fraction and a fraction of lipids derived from Escherichia coli outer membrane vesicles (OMVs). We exploit the enhanced resolution and molecular specificity that SIM can offer to identify areas of interest in these bilayers and the atomic scale resolution that the AFM provides to create detailed topography maps of the bilayers. We are thus able to understand the way in which the two different lipid fractions (natural and synthetic) mix within the bilayers, quantify the amount of bacterial membrane incorporated in the bilayer and directly visualise the interaction of these bilayers with bacteria-specific, membrane-binding proteins. Our work sets the foundation for accurately understanding the composition and properties of OMV-derived SLBs and establishes correlative AFM/ SIM as a method for characterising complex systems at the nanoscale.

scholarly journals Nanoparticle-Lipid Interaction: Job Scattering Plots to Differentiate Vesicle Aggregation from Supported Lipid Bilayer Formation

2018 ◽  
Vol 2 (4) ◽  
pp. 50 ◽  
Author(s):  
Fanny Mousseau ◽  
Evdokia Oikonomou ◽  
Victor Baldim ◽  
Stéphane Mornet ◽  
Jean-François Berret
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Living Cells ◽  
Supported Lipid Bilayers ◽  
Supported Lipid Bilayer ◽  
Mixed Aggregates ◽  
Method Of Continuous Variation ◽  
The Impact ◽  
Analytical Expressions ◽  
Lipid Interaction

The impact of nanomaterials on lung fluids, or on the plasma membrane of living cells, has prompted researchers to examine the interactions between nanoparticles and lipid vesicles. Recent studies have shown that nanoparticle-lipid interaction leads to a broad range of structures including supported lipid bilayers (SLB), particles adsorbed at the surface or internalized inside vesicles, and mixed aggregates. Currently, there is a need to have simple protocols that can readily evaluate the structures made from particles and vesicles. Here we apply the method of continuous variation for measuring Job scattering plots and provide analytical expressions for the scattering intensity in various scenarios. The result that emerges from the comparison between experiments and modeling is that electrostatics play a key role in the association, but it is not sufficient to induce the formation of supported lipid bilayers.

scholarly journals IR-MALDESI Mass Spectrometry Imaging at 50 Micron Spatial Resolution

2017 ◽  
Vol 28 (10) ◽  
pp. 2099-2107 ◽  
Author(s):  
Mark T. Bokhart ◽  
Jeffrey Manni ◽  
Kenneth P. Garrard ◽  
Måns Ekelöf ◽  
Milad Nazari ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging

scholarly journals Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers

2010 ◽  
Vol 98 (3) ◽  
pp. 673a
Author(s):  
Lisa V. Simonsson ◽  
Peter Jönsson ◽  
Gudrun Stengel ◽  
Fredrik Höök
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Supported Lipid Bilayers ◽  
Site Specific ◽  
Controlled Fusion

A Simple Method for Improving the Spatial Resolution in Infrared Laser Ablation Mass Spectrometry Imaging

2017 ◽  
Vol 28 (6) ◽  
pp. 1060-1065 ◽  
Author(s):  
Juha-Pekka Hieta ◽  
Anu Vaikkinen ◽  
Samuli Auno ◽  
Heikki Räikkönen ◽  
Markus Haapala ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Infrared Laser ◽  
Simple Method
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