Effects of temperature and novobiocin on the expression of calf prochymosin gene and on plasmid copy number in recombinantEscherichia coli

1998 ◽  
Vol 43 (1) ◽  
pp. 63-67 ◽  
Author(s):  
F. Kaprálek ◽  
P. J. Tichý ◽  
M. Fábry ◽  
J. Sedláček
Keyword(s):  
Copy Number ◽  
Plasmid Copy Number ◽  
Plasmid Copy ◽  
Effects Of Temperature

scholarly journals Segregational Drift and the Interplay between Plasmid Copy Number and Evolvability

2018 ◽  
Vol 36 (3) ◽  
pp. 472-486 ◽  
Author(s):  
Judith Ilhan ◽  
Anne Kupczok ◽  
Christian Woehle ◽  
Tanita Wein ◽  
Nils F Hülter ◽  
...  
Keyword(s):  
Copy Number ◽  
Plasmid Copy Number ◽  
Plasmid Copy

scholarly journals Plasmid copy number noise in monoclonal populations of bacteria

2010 ◽  
Vol 81 (1) ◽  
Author(s):  
Jérôme Wong Ng ◽  
Didier Chatenay ◽  
Jérôme Robert ◽  
Michael Guy Poirier
Keyword(s):  
Copy Number ◽  
Plasmid Copy Number ◽  
Plasmid Copy

scholarly journals Effects of Biofilm Growth on Plasmid Copy Number and Expression of Antibiotic Resistance Genes in Enterococcus faecalis

2013 ◽  
Vol 57 (4) ◽  
pp. 1850-1856 ◽  
Author(s):  
L. C. Cook ◽  
G. M. Dunny
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Resistance Genes ◽  
Copy Number ◽  
Antibiotic Resistance Genes ◽  
Plasmid Copy Number ◽  
Plasmid Copy ◽  
Rapid Reduction ◽  
Biofilm Growth ◽  
Content Type

ABSTRACTBiofilm growth causes increased average plasmid copy number as well as increased copy number heterogeneity inEnterococcus faecaliscells carrying plasmid pCF10. In this study, we examined whether biofilm growth affected the copy number and expression of antibiotic resistance determinants for several plasmids with diverse replication systems. Four differentE. faecalisplasmids, unrelated to pCF10, demonstrated increased copy number in biofilm cells. In biofilm cells, we also observed increased transcription of antibiotic resistance genes present on these plasmids. The increase in plasmid copy number correlated with increased plating efficiency on high concentrations of antibiotics. Single-cell analysis of strains carrying two different plasmids suggested that the increase in plasmid copy number associated with biofilm growth was restricted to a subpopulation of biofilm cells. Regrowth of harvested biofilm cells in liquid culture resulted in a rapid reduction of plasmid copy number to that observed in the planktonic state. These results suggest a possible mechanism by which biofilm growth could reduce susceptibility to antibiotics in clinical settings.

scholarly journals Determination of Plasmid Copy Number Reveals the Total Plasmid DNA Amount Is Greater than the Chromosomal DNA Amount in Bacillus thuringiensis YBT-1520

PLoS ONE ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. e16025 ◽  
Author(s):  
Chunying Zhong ◽  
Donghai Peng ◽  
Weixing Ye ◽  
Lujun Chai ◽  
Junliang Qi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Plasmid Dna ◽  
Copy Number ◽  
Plasmid Copy Number ◽  
Chromosomal Dna ◽  
Dna Amount ◽  
Plasmid Copy

scholarly journals Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli

2006 ◽  
Vol 123 (3) ◽  
pp. 273-280 ◽  
Author(s):  
Changsoo Lee ◽  
Jaai Kim ◽  
Seung Gu Shin ◽  
Seokhwan Hwang
Keyword(s):  
Escherichia Coli ◽  
Copy Number ◽  
Plasmid Copy Number ◽  
Plasmid Copy ◽  
Qpcr Quantification
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