Effect of dilution rate on growth, productivity, cell cycle and size, and shear sensitivity of a hybridoma cell in a continuous culture

1993 ◽  
Vol 41 (4) ◽  
pp. 429-439 ◽  
Author(s):  
D. E. Martens ◽  
C. D. de Gooijer ◽  
C. A. M. van der Velden-de Groot ◽  
E. C. Beuvery ◽  
J. Tramper
Keyword(s):  
Cell Cycle ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Shear Sensitivity

CONTINUOUS CULTURE OF BRUCELLA ABORTUS S.19

1961 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
Andreas H. W. Hauschild ◽  
Hilliard Pivnick
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Brucella Abortus ◽  
Specific Growth ◽  
Limiting Factor ◽  
Continuous Growth ◽  
Viable Cells ◽  
Metabolic Products ◽  
Growth Of Bacteria

An apparatus is described for the continuous growth of bacteria. Brucella abortus S.19 has been grown in continuous culture for periods up to 3 weeks with populations up to 2 × 1011viable cells per ml and without the establishment of nonsmooth variants.Concentrations between 3 × 109and 2 × 1011cells per ml could be maintained as a function of the dilution rate without the requirement of a known limiting factor in the medium. In a series of steady-state conditions, the specific growth rate increased steadily up to 0.28 hour−1with decreasing population levels.Incidence of mutants was governed by the dilution rate and could also be reduced by various chelating substances.In continuous growth combined with continuous dialysis, population levels were approximately twice those obtained in continuous growth without dialysis. The effect of dialysis appears to be the continuous removal of growth-limiting metabolic products.

scholarly journals Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 157
Author(s):  
Idan Koren ◽  
Sammy Boussiba ◽  
Inna Khozin-Goldberg ◽  
Aliza Zarka
Keyword(s):  
Cell Cycle ◽  
Continuous Culture ◽  
Continuous Cultivation ◽  
High Light ◽  
Nuclear Transformation ◽  
Storage Lipids ◽  
Microalgal Culture ◽  
Multiple Fission ◽  
Cell Release ◽  
Chromochloris Zofingiensis

Several green algae can divide by multiple fission and spontaneously synchronize their cell cycle with the available light regime. The yields that can be obtained from a microalgal culture are directly affected by cell cycle events. Chromochloris zofingiensis is considered as one of the most promising microalgae for biotechnological applications due to its fast growth and the flexible trophic capabilities. It is intensively investigated in the context of bio-commodities production (carotenoids, storage lipids); however, the pattern of cell-cycle events under common cultivation strategies was not yet characterized for C. zofingiensis. In this study, we have employed fluorescence microscopy to characterize the basic cell-cycle dynamics under batch and continuous modes of phototrophic C. zofingiensis cultivation. Staining with SYBR green—applied in DMSO solution—enabled, for the first time, the clear and simple visualization of polynuclear stages in this microalga. Accordingly, we concluded that C. zofingiensis divides by a consecutive pattern of multiple fission, whereby it spontaneously synchronizes growth and cell division according to the available illumination regime. In high-light continuous culture or low-light batch culture, C. zofingiensis cell-cycle was completed within several light-dark (L/D) cycles (14 h/10 h); however, cell divisions were synchronized with the dark periods only in the high-light continuous culture. In both modes of cultivation, daughter cell release was mainly facilitated by division of 8 and 16-polynuclear cells. The results of this study are of both fundamental and applied science significance and are also important for the development of an efficient nuclear transformation system for C. zofingiensis.

scholarly journals Effect of dilution rate on lipopolysaccharide and serum resistance of Neisseria gonorrhoeae grown in continuous culture.

1983 ◽  
Vol 41 (1) ◽  
pp. 74-82 ◽  
Author(s):  
S A Morse ◽  
C S Mintz ◽  
S K Sarafian ◽  
L Bartenstein ◽  
M Bertram ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Serum Resistance

scholarly journals Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge

2006 ◽  
Vol 49 (6) ◽  
pp. 873-880 ◽  
Author(s):  
Leila Larisa Medeiros Marques ◽  
João Batista Buzato ◽  
Maria Antonia Pedrine Colabone Celligoi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Culture ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Immobilized Cells ◽  
Invertase Activity ◽  
Free Cell ◽  
Immobilized Cell ◽  
Luffa Cylindrica ◽  
Ultrasound Application

This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These results showed that ultrasound application in continuous culture could have great potential for application in biotechnological techniques.

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