Effect of dilution rate and methanol-glycerol mixed feeding on heterologousRhizopus oryzaelipase production withPichia pastorisMut+phenotype in continuous culture

2015 ◽  
Vol 31 (3) ◽  
pp. 707-714 ◽  
Author(s):  
Christian Canales ◽  
Claudia Altamirano ◽  
Julio Berrios
Keyword(s):  
Continuous Culture ◽  
Dilution Rate ◽  
Mixed Feeding

CONTINUOUS CULTURE OF BRUCELLA ABORTUS S.19

1961 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
Andreas H. W. Hauschild ◽  
Hilliard Pivnick
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Brucella Abortus ◽  
Specific Growth ◽  
Limiting Factor ◽  
Continuous Growth ◽  
Viable Cells ◽  
Metabolic Products ◽  
Growth Of Bacteria

An apparatus is described for the continuous growth of bacteria. Brucella abortus S.19 has been grown in continuous culture for periods up to 3 weeks with populations up to 2 × 1011viable cells per ml and without the establishment of nonsmooth variants.Concentrations between 3 × 109and 2 × 1011cells per ml could be maintained as a function of the dilution rate without the requirement of a known limiting factor in the medium. In a series of steady-state conditions, the specific growth rate increased steadily up to 0.28 hour−1with decreasing population levels.Incidence of mutants was governed by the dilution rate and could also be reduced by various chelating substances.In continuous growth combined with continuous dialysis, population levels were approximately twice those obtained in continuous growth without dialysis. The effect of dialysis appears to be the continuous removal of growth-limiting metabolic products.

scholarly journals Effect of dilution rate on lipopolysaccharide and serum resistance of Neisseria gonorrhoeae grown in continuous culture.

1983 ◽  
Vol 41 (1) ◽  
pp. 74-82 ◽  
Author(s):  
S A Morse ◽  
C S Mintz ◽  
S K Sarafian ◽  
L Bartenstein ◽  
M Bertram ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Serum Resistance

Effect of dilution rate on growth, productivity, cell cycle and size, and shear sensitivity of a hybridoma cell in a continuous culture

1993 ◽  
Vol 41 (4) ◽  
pp. 429-439 ◽  
Author(s):  
D. E. Martens ◽  
C. D. de Gooijer ◽  
C. A. M. van der Velden-de Groot ◽  
E. C. Beuvery ◽  
J. Tramper
Keyword(s):  
Cell Cycle ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Shear Sensitivity

scholarly journals Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge

2006 ◽  
Vol 49 (6) ◽  
pp. 873-880 ◽  
Author(s):  
Leila Larisa Medeiros Marques ◽  
João Batista Buzato ◽  
Maria Antonia Pedrine Colabone Celligoi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Culture ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Immobilized Cells ◽  
Invertase Activity ◽  
Free Cell ◽  
Immobilized Cell ◽  
Luffa Cylindrica ◽  
Ultrasound Application

This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These results showed that ultrasound application in continuous culture could have great potential for application in biotechnological techniques.

scholarly journals Coevolution of Bacteriophage PP01 and Escherichia coli O157:H7 in Continuous Culture

2003 ◽  
Vol 69 (1) ◽  
pp. 170-176 ◽  
Author(s):  
Katsunori Mizoguchi ◽  
Masatomo Morita ◽  
Curt R. Fischer ◽  
Masatoshi Yoichi ◽  
Yasunori Tanji ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Escape Mutant ◽  
E Coli ◽  
Escape Mutants ◽  
Low Efficiency

ABSTRACT The interaction between Escherichia coli O157:H7 and its specific bacteriophage PP01 was investigated in chemostat continuous culture. Following the addition of bacteriophage PP01, E. coli O157:H7 cell lysis was observed by over 4 orders of magnitude at a dilution rate of 0.876 h−1 and by 3 orders of magnitude at a lower dilution rate (0.327 h−1). However, the appearance of a series of phage-resistant E. coli isolates, which showed a low efficiency of plating against bacteriophage PP01, led to an increase in the cell concentration in the culture. The colony shape, outer membrane protein expression, and lipopolysaccharide production of each escape mutant were compared. Cessation of major outer membrane protein OmpC production and alteration of lipopolysaccharide composition enabled E. coli O157:H7 to escape PP01 infection. One of the escape mutants of E. coli O157:H7 which formed a mucoid colony (Mu) on Luria-Bertani agar appeared 56 h postincubation at a dilution rate of 0.867 h−1 and persisted until the end of the experiment (∼200 h). Mu mutant cells could coexist with bacteriophage PP01 in batch culture. Concentrations of the Mu cells and bacteriophage PP01 increased together. The appearance of mutant phage, which showed a different host range among the O157:H7 escape mutants than wild-type PP01, was also detected in the chemostat culture. Thus, coevolution of phage and E. coli O157:H7 proceeded as a mutual arms race in chemostat continuous culture.

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