Measuring the pH environment of DNA delivered using nonviral vectors: Implications for lysosomal trafficking

2002 ◽  
Vol 78 (5) ◽  
pp. 503-508 ◽  
Author(s):  
Akin Akinc ◽  
Robert Langer
Keyword(s):  
Nonviral Vectors ◽  
Lysosomal Trafficking

scholarly journals Biopolymeric Materials Used as Nonviral Vectors: A Review

2021 ◽  
Vol 2 (1) ◽  
pp. 100-109
Author(s):  
Jailson de Araújo Santos ◽  
Daniel Barbosa Liarte ◽  
Alessandra Braga Ribeiro ◽  
Marcia dos Santos Rizzo ◽  
Marcília Pinheiro da Costa ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Web Of Science ◽  
Gene Transfection ◽  
Genetic Material ◽  
Nonviral Vectors ◽  
Cationic Polymers ◽  
Genetic Trait ◽  
Low Diversity ◽  
Different Types ◽  
Materials Used

Bacterial transformation and gene transfection can be understood as being the results of introducing specific genetic material into cells, resulting in gene expression, and adding a new genetic trait to the host cell. Many studies have been carried out to investigate different types of lipids and cationic polymers as promising nonviral vectors for DNA transfer. The present study aimed to carry out a systematic review on the use of biopolymeric materials as nonviral vectors. The methodology was carried out based on searches of scientific articles and applications for patents published or deposited from 2006 to 2020 in different databases for patents (EPO, USPTO, and INPI) and articles (Scopus, Web of Science, and Scielo). The results showed that there are some deposits of patents regarding the use of chitosan as a gene carrier. The 16 analyzed articles allowed us to infer that the use of biopolymers as nonviral vectors is limited due to the low diversity of biopolymers used for these purposes. It was also observed that the use of different materials as nonviral vectors is based on chemical structure modifications of the material, mainly by the addition of cationic groups. Thus, the use of biopolymers as nonviral vectors is still limited to only a few polysaccharide types, emphasizing the need for further studies involving the use of different biopolymers in processes of gene transfer.

scholarly journals Complete tumor prevention by engineered tumor cell vaccines employing nonviral vectors

2003 ◽  
Vol 10 (12) ◽  
pp. 887-897 ◽  
Author(s):  
Inés Moret-Tatay ◽  
Joaquín Díaz ◽  
Francisco M Marco ◽  
Antonio Crespo ◽  
Salvador F Aliño
Keyword(s):  
Tumor Cell ◽  
Nonviral Vectors ◽  
Tumor Prevention ◽  
Tumor Cell Vaccines ◽  
Complete Tumor

Chitosan-Based Nonviral Vectors for Gene Delivery

2005 ◽  
pp. 817-836
Author(s):  
Wen Guang Liu ◽  
William Lu ◽  
Kang De Yao
Keyword(s):  
Gene Delivery ◽  
Nonviral Vectors

scholarly journals Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor–mediated inflammatory responses

2016 ◽  
Vol 214 (1) ◽  
pp. 227-244 ◽  
Author(s):  
Andreas Westphal ◽  
Weijia Cheng ◽  
Jinbo Yu ◽  
Guntram Grassl ◽  
Martina Krautkrämer ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Membrane Trafficking ◽  
Inflammatory Responses ◽  
Functional Integration ◽  
Receptor Signaling ◽  
Toll Like Receptor ◽  
Tlr Signaling ◽  
Lysosomal Trafficking ◽  
Phagosomal Maturation ◽  
Chediak Higashi Syndrome

Subcellular compartmentalization of receptor signaling is an emerging principle in innate immunity. However, the functional integration of receptor signaling pathways into membrane trafficking routes and its physiological relevance for immune responses is still largely unclear. In this study, using Lyst-mutant beige mice, we show that lysosomal trafficking regulator Lyst links endolysosomal organization to the selective control of toll-like receptor 3 (TLR3)– and TLR4-mediated proinflammatory responses. Consequently, Lyst-mutant mice showed increased susceptibility to bacterial infection and were largely resistant to endotoxin-induced septic shock. Mechanistic analysis revealed that Lyst specifically controls TLR3- and TLR4-induced endosomal TRIF (TIR domain–containing adapter-inducing interferon β) signaling pathways. Loss of functional Lyst leads to dysregulated phagosomal maturation, resulting in a failure to form an activation-induced Rab7+ endosomal/phagosomal compartment. This specific Rab7+ compartment was further demonstrated to serve as a major site for active TRIF signaling events, thus linking phagosomal maturation to specific TLR signaling pathways. The immunoregulatory role of Lyst on TLR signaling pathways was confirmed in human cells by CRISPR/Cas9-mediated gene inactivation. As mutations in LYST cause human Chédiak-Higashi syndrome, a severe immunodeficiency, our findings also contribute to a better understanding of human disease mechanisms.

scholarly journals Poly(β-amino ester)-co -poly(caprolactone) Terpolymers as Nonviral Vectors for mRNA Delivery In Vitro and In Vivo

2018 ◽  
Vol 7 (14) ◽  
pp. 1800249 ◽  
Author(s):  
Umberto Capasso Palmiero ◽  
James C. Kaczmarek ◽  
Owen S. Fenton ◽  
Daniel G. Anderson
Keyword(s):  
Nonviral Vectors ◽  
Amino Ester ◽  
Poly Caprolactone

Viral and Nonviral Vectors for In Vivo and Ex Vivo Gene Therapies

2016 ◽  
pp. 155-177 ◽  
Author(s):  
A. Crespo-Barreda ◽  
M.M. Encabo-Berzosa ◽  
R. González-Pastor ◽  
P. Ortíz-Teba ◽  
M. Iglesias ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Nonviral Vectors ◽  
Gene Therapies

The lysosomal trafficking regulator is necessary for normal wound healing

2021 ◽  
Author(s):  
Jacob C. Zbinden ◽  
Gabriel J. M. Mirhaidari ◽  
Kevin M. Blum ◽  
Andrew J. Musgrave ◽  
James W. Reinhardt ◽  
...  
Keyword(s):  
Wound Healing ◽  
Lysosomal Trafficking ◽  
Normal Wound Healing

Dramatic influence of the hydroxy functionality of azasugar moiety in the head group region of tocopherol-based cationic lipids on in vitro gene transfection efficacies

2021 ◽  
Author(s):  
Venkanna Muripiti ◽  
Brijesh Lohchania ◽  
Venkatesh Ravula ◽  
Shireesha Manturthi ◽  
Srujan Marepally ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Lipids ◽  
Head Group ◽  
Nonviral Vectors

Cationic lipids have been effectively used as nonviral vectors for the delivery of polynucleic acids into the cytosol.

scholarly journals Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaolai Zhou ◽  
Lirong Sun ◽  
Oliver Bracko ◽  
Ji Whae Choi ◽  
Yan Jia ◽  
...  
Keyword(s):  
Frontotemporal Lobar Degeneration ◽  
Lysosomal Trafficking
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