scholarly journals Poly(β-amino ester)-co -poly(caprolactone) Terpolymers as Nonviral Vectors for mRNA Delivery In Vitro and In Vivo

2018 ◽  
Vol 7 (14) ◽  
pp. 1800249 ◽  
Author(s):  
Umberto Capasso Palmiero ◽  
James C. Kaczmarek ◽  
Owen S. Fenton ◽  
Daniel G. Anderson
Keyword(s):  
Nonviral Vectors ◽  
Amino Ester ◽  
Poly Caprolactone

scholarly journals Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing

2019 ◽  
Vol 5 (12) ◽  
pp. eaay3255 ◽  
Author(s):  
Yuan Rui ◽  
David R. Wilson ◽  
John Choi ◽  
Mahita Varanasi ◽  
Katie Sanders ◽  
...  
Keyword(s):  
Protein Delivery ◽  
Gene Editing ◽  
Gene Knockout ◽  
Cell Types ◽  
Endosomal Escape ◽  
Biological Research ◽  
Amino Ester ◽  
Cytosolic Protein

Efficient cytosolic protein delivery is necessary to fully realize the potential of protein therapeutics. Current methods of protein delivery often suffer from low serum tolerance and limited in vivo efficacy. Here, we report the synthesis and validation of a previously unreported class of carboxylated branched poly(β-amino ester)s that can self-assemble into nanoparticles for efficient intracellular delivery of a variety of different proteins. In vitro, nanoparticles enabled rapid cellular uptake, efficient endosomal escape, and functional cytosolic protein release into cells in media containing 10% serum. Moreover, nanoparticles encapsulating CRISPR-Cas9 ribonucleoproteins (RNPs) induced robust levels of gene knock-in (4%) and gene knockout (>75%) in several cell types. A single intracranial administration of nanoparticles delivering a low RNP dose (3.5 pmol) induced robust gene editing in mice bearing engineered orthotopic murine glioma tumors. This self-assembled polymeric nanocarrier system enables a versatile protein delivery and gene editing platform for biological research and therapeutic applications.

In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site‐Specific Controlled Drug Release

2019 ◽  
Vol 220 (17) ◽  
pp. 1900188
Author(s):  
Vuk V. Filipović ◽  
Marija M. Babić ◽  
Dejan Gođevac ◽  
Aleksandar Pavić ◽  
Jasmina Nikodinović‐Runić ◽  
...  
Keyword(s):  
Drug Release ◽  
Controlled Drug Release ◽  
Amino Ester ◽  
Site Specific

In vitro and in vivo studies of biaxially electrospun poly(caprolactone)/gelatin nanofibers, reinforced with cellulose nanocrystals, for wound healing applications

Cellulose ◽  
2020 ◽  
Vol 27 (9) ◽  
pp. 5179-5196
Author(s):  
Ahmad Hivechi ◽  
S. Hajir Bahrami ◽  
Ronald A. Siegel ◽  
Peiman B.Milan ◽  
Moein Amoupour
Keyword(s):  
Wound Healing ◽  
Cellulose Nanocrystals ◽  
In Vivo Studies ◽  
Poly Caprolactone

Cationic phosphonolipids as nonviral vectors: In vitro and in vivo applications

2000 ◽  
Vol 89 (5) ◽  
pp. 629
Author(s):  
P. Delépine ◽  
C. Guillaume ◽  
V. Floch ◽  
S. Loisel ◽  
J.-J. Yaouanc ◽  
...  
Keyword(s):  
Nonviral Vectors

Silk fibroin and poly-caprolactone vascular grafts tested in vitro and in vivo mimic natural vessel

2015 ◽  
Vol 44 ◽  
pp. e246
Author(s):  
S.G. Kim ◽  
A.R. Park ◽  
Y.H. Park ◽  
H.J. Kim ◽  
M.K. Kim ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Vascular Grafts ◽  
Poly Caprolactone

scholarly journals Poly(β-amino ester) Nanoparticle Delivery of TP53 Has Activity against Small Cell Lung Cancer In Vitro and In Vivo

2013 ◽  
Vol 12 (4) ◽  
pp. 405-415 ◽  
Author(s):  
Chandrashekhar D. Kamat ◽  
Ron B. Shmueli ◽  
Nick Connis ◽  
Charles M. Rudin ◽  
Jordan J. Green ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Amino Ester ◽  
Small Cell Lung ◽  
Nanoparticle Delivery

scholarly journals ANTI-ALZHEIMER ACTIVITY OF TACRINE LOADED METHOXYPOLY (ETHYLENE GLYCOL) POLY (CAPROLACTONE) NANOPARTICLES

2018 ◽  
Vol 11 ◽  
Author(s):  
Felix Joe V ◽  
Sathesh Kumar S
Keyword(s):  
Ethylene Glycol ◽  
Therapeutic Efficacy ◽  
Laser Scanning ◽  
Amyloid Β ◽  
Cytotoxic Effects ◽  
Morris Water Maze Task ◽  
Control Step ◽  
Poly Caprolactone

Objective: The objective of the study was to study the in vitro cytotoxic effects of amyloid-β (Aβ) in SH-SY5Y cells and in vivo therapeutic efficacy of drug-loaded nanoparticles (TMPCN-3) in mice.Methods: In vitro cytotoxic effects of Aβ in SH-SY5Y cells were carried out using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay; cellular uptake study was carried using a laser scanning fluorescence microscope. In vivo therapeutic efficacy of drug loaded nanoparticles, optimized formulation (TMPCN-3) in mice. Animals (n=18) were divided into three different groups each consisting of six animals in each group.Results: Treatment with Aβ-induced cytotoxicity showed a 54% decrease in cell viability. Drug-treated with TMPCN-3 showed greater intracellular accumulation. In the invasive study TMPCN-3 nanoparticles, the SH-SY5Y cells (73%) were decreased when compared to the control cells (91%). In vivo therapeutic efficacy of drug-loaded nanoparticles was studied in mice, Morris water maze task shows polymeric nanoformulations indicates critical change execution (expanded memory maintenance) when contrasted with control, step down condition maintains a considered space from response created by the memory change and polymeric nanoformulations, and after that taken by tacrine and control the quantity of errors are higher. The nanoformulations distributed massive decreasing in acetylcholine esterase level brain homogenate.Conclusion: Tacrine-loaded methoxypoly(ethylene glycol) poly(caprolactone) reveals that the in vitro cell line believes emphasized the reduced risk of tacrine loaded nanoparticles as there is a significant decrease half maximal inhibitory concentration and higher convention of drug in the glial cells and by in vivo reflects was confirmed that nanoformulation indicated improved memory.

Prevention of abdominal adhesion by a polycaprolactone/phospholipid hybrid film containing quercetin and Ag nanoparticles

Nanomedicine ◽  
2021 ◽  
Author(s):  
Reza Hosseinpour-Moghadam ◽  
Shahram Rabbani ◽  
Arash Mahboubi ◽  
Sayyed Abbas Tabatabai ◽  
Azadeh Haeri
Keyword(s):  
Sustained Release ◽  
Histochemical Staining ◽  
In Vivo Studies ◽  
In Vitro Tests ◽  
Potential Candidate ◽  
Control Groups ◽  
Coating Films ◽  
Poly Caprolactone

Aim: To develop quercetin-loaded poly(caprolactone) (PCL)/soybean phosphatidylcholine (PC) films coated with silver (Ag) to prevent the formation of postoperative adhesions (POA). Materials & methods: Films were prepared using the solvent casting method, coated with Ag, and underwent  in vitro tests. In vivo studies were conducted employing an animal model of sidewall defect and cecum abrasion. Results: Films showed sustained release behavior of quercetin and Ag. Coating films with Ag improved their antimicrobial activity. In vivo studies confirmed superior antiadhesion properties of films compared with the control groups evaluated by gross observation, histochemical staining and immunohistochemistry analyses. Conclusion: Ag-Q-PCL-PC films are a potential candidate to prevent POA by acting as a sustained release delivery system and physical barrier.

scholarly journals Cationic Phosphonolipids as Nonviral Vectors: In Vitro and in Vivo Applications

2000 ◽  
Vol 89 (5) ◽  
pp. 629-638 ◽  
Author(s):  
P. Delépine ◽  
C. Guillaume ◽  
V. Floch ◽  
S. Loisel ◽  
J.‐J. Yaouanc ◽  
...  
Keyword(s):  
Nonviral Vectors
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