The lysosomal trafficking regulator is necessary for normal wound healing

2021 ◽  
Author(s):  
Jacob C. Zbinden ◽  
Gabriel J. M. Mirhaidari ◽  
Kevin M. Blum ◽  
Andrew J. Musgrave ◽  
James W. Reinhardt ◽  
...  
Keyword(s):  
Wound Healing ◽  
Lysosomal Trafficking ◽  
Normal Wound Healing

scholarly journals Xanthine Oxidoreductase Function Contributes to Normal Wound Healing

2015 ◽  
Vol 21 (1) ◽  
pp. 313-322 ◽  
Author(s):  
Michael C. Madigan ◽  
Ryan M. McEnaney ◽  
Ankur J. Shukla ◽  
Guiying Hong ◽  
Eric E. Kelley ◽  
...  
Keyword(s):  
Wound Healing ◽  
Xanthine Oxidoreductase ◽  
Normal Wound Healing

Single layer suturing in intracapsular myomectomy of intramural myomas is sufficient for a normal wound healing

2020 ◽  
Vol 248 ◽  
pp. 204-210
Author(s):  
Yannis Prapas ◽  
Athanasios Zikopoulos ◽  
Stamatios Petousis ◽  
Panayotis Xiromeritis ◽  
Andrea Tinelli ◽  
...  
Keyword(s):  
Wound Healing ◽  
Single Layer ◽  
Normal Wound Healing

scholarly journals Functional Mining of the Crotalus Spp. Venom Protease Repertoire Reveals Potential for Chronic Wound Therapeutics

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3401
Author(s):  
David Meléndez-Martínez ◽  
Luis Fernando Plenge-Tellechea ◽  
Ana Gatica-Colima ◽  
Martha Sandra Cruz-Pérez ◽  
José Manuel Aguilar-Yáñez ◽  
...  
Keyword(s):  
Wound Healing ◽  
Serine Proteases ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Healing Process ◽  
Wound Healing Process ◽  
Major Health Problem ◽  
Normal Wound Healing ◽  
Alternative Sources ◽  
Fibrin Clots

Chronic wounds are a major health problem that cause millions of dollars in expenses every year. Among all the treatments used, active wound treatments such as enzymatic treatments represent a cheaper and specific option with a fast growth category in the market. In particular, bacterial and plant proteases have been employed due to their homology to human proteases, which drive the normal wound healing process. However, the use of these proteases has demonstrated results with low reproducibility. Therefore, alternative sources of proteases such as snake venom have been proposed. Here, we performed a functional mining of proteases from rattlesnakes (Crotalus ornatus, C. molossus nigrescens, C. scutulatus, and C. atrox) due to their high protease predominance and similarity to native proteases. To characterize Crotalus spp. Proteases, we performed different protease assays to measure and confirm the presence of metalloproteases and serine proteases, such as the universal protease assay and zymography, using several substrates such as gelatin, casein, hemoglobin, L-TAME, fibrinogen, and fibrin. We found that all our venom extracts degraded casein, gelatin, L-TAME, fibrinogen, and fibrin, but not hemoglobin. Crotalus ornatus and C. m. nigrescens extracts were the most proteolytic venoms among the samples. Particularly, C. ornatus predominantly possessed low molecular weight proteases (P-I metalloproteases). Our results demonstrated the presence of metalloproteases capable of degrading gelatin (a collagen derivative) and fibrin clots, whereas serine proteases were capable of degrading fibrinogen-generating fibrin clots, mimicking thrombin activity. Moreover, we demonstrated that Crotalus spp. are a valuable source of proteases that can aid chronic wound-healing treatments.

Suppressor cell generation during normal wound healing

1988 ◽  
Vol 44 (4) ◽  
pp. 321-325 ◽  
Author(s):  
Robert J. Breslin ◽  
Hannah L. Wasserkrug ◽  
Gershon Efron ◽  
Adrian Barbul
Keyword(s):  
Wound Healing ◽  
Suppressor Cell ◽  
Cell Generation ◽  
Normal Wound Healing

scholarly journals From Grafts to Human Bioengineered Vascularized Skin Substitutes

2020 ◽  
Vol 21 (21) ◽  
pp. 8197
Author(s):  
Wasima Oualla-Bachiri ◽  
Ana Fernández-González ◽  
María I. Quiñones-Vico ◽  
Salvador Arias-Santiago
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Chronic Wounds ◽  
Thermal Regulation ◽  
Skin Substitute ◽  
Skin Substitutes ◽  
Normal Wound Healing ◽  
Physiological Homeostasis ◽  
Promising Solution ◽  
Native Skin

The skin plays an important role in the maintenance of the human’s body physiological homeostasis. It acts as a coverage that protects against infective microorganism or biomechanical impacts. Skin is also implied in thermal regulation and fluid balance. However, skin can suffer several damages that impede normal wound-healing responses and lead to chronic wounds. Since the use of autografts, allografts, and xenografts present source limitations and intense rejection associated problems, bioengineered artificial skin substitutes (BASS) have emerged as a promising solution to address these problems. Despite this, currently available skin substitutes have many drawbacks, and an ideal skin substitute has not been developed yet. The advances that have been produced on tissue engineering techniques have enabled improving and developing new arising skin substitutes. The aim of this review is to outline these advances, including commercially available skin substitutes, to finally focus on future tissue engineering perspectives leading to the creation of autologous prevascularized skin equivalents with a hypodermal-like layer to achieve an exemplary skin substitute that fulfills all the biological characteristics of native skin and contributes to wound healing.

Viral Infections and Wounds

1998 ◽  
Vol 6 (3) ◽  
pp. 159-161 ◽  
Author(s):  
Naznin Karsan ◽  
Ronald M Zuker
Keyword(s):  
Wound Healing ◽  
Case Report ◽  
Detrimental Effect ◽  
Viral Infections ◽  
Healing Process ◽  
Wound Healing Process ◽  
Normal Wound Healing

The effects of viral infections on normal wound healing are discussed. The case report presented suggests that viruses can have a detrimental effect on the normal wound healing process.

Role of Nitric Oxide in Wound Healing

2002 ◽  
Vol 4 (1) ◽  
pp. 5-15 ◽  
Author(s):  
Beverly B. Childress ◽  
Joyce K. Stechmiller
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Growth Factors ◽  
Animal Studies ◽  
Chronic Wounds ◽  
Current Knowledge ◽  
Wound Care ◽  
Impaired Wound Healing ◽  
Normal Wound Healing ◽  
Age Related

Chronic wounds mainly affect elderly individuals and persons with comorbid diseases due to a compromised immune status. An age-related decline in immune function deters proper healing of wounds in an orderly and timely manner. Thus, older adults with 1 or more concomitant illnesses are more likely to experience and suffer from a nonhealing wound, which may drastically decrease their quality of life and financial resources. Novel therapies in wound care management rely heavily on our current knowledge of wound healing physiology. It is well established that normal wound healing occurs sequentially and is strictly regulated by pro-inflammatory cytokines and growth factors. A multitude of commercial products such as growth factors are available; however, their effectiveness in healing chronic wounds has yet to be proven. Recently, investigators have implicated nitric oxide (NO) in the exertion of regulatory forces on various cellular activities of the inflammatory and proliferative phases of wound healing. Gene therapy in animal studies has shown promising results and is furthering our understanding of impaired wound healing. The purpose of this article is to review the literature on NO and its role in wound healing. A discussion of the physiology of normal healing and the pathophysiology of chronic wounds is provided.

scholarly journals Normal Wound Healing in Mice Deficient for Fibulin-5, an Elastin Binding Protein Essential for Dermal Elastic Fiber Assembly

2006 ◽  
Vol 126 (12) ◽  
pp. 2707-2714 ◽  
Author(s):  
Qian Zheng ◽  
Jiwon Choi ◽  
Leonie Rouleau ◽  
Richard L. Leask ◽  
James A. Richardson ◽  
...  
Keyword(s):  
Wound Healing ◽  
Binding Protein ◽  
Elastic Fiber ◽  
Fiber Assembly ◽  
Normal Wound Healing ◽  
Elastin Binding Protein

scholarly journals Shedding of microparticles by myofibroblasts as mediator of cellular cross-talk during normal wound healing

2010 ◽  
Vol 225 (3) ◽  
pp. 734-740 ◽  
Author(s):  
Véronique J. Moulin ◽  
Dominique Mayrand ◽  
Hugo Messier ◽  
Maria Carmen Martinez ◽  
Carlos A. Lopez-Vallé ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cross Talk ◽  
Normal Wound Healing

scholarly journals Reduction of ARNT in myeloid cells causes immune suppression and delayed wound healing

2014 ◽  
Vol 307 (4) ◽  
pp. C349-C357 ◽  
Author(s):  
Christopher Scott ◽  
James Bonner ◽  
Danqing Min ◽  
Philip Boughton ◽  
Rebecca Stokes ◽  
...  
Keyword(s):  
Wound Healing ◽  
Myeloid Cells ◽  
Myeloid Cell ◽  
Delayed Wound Healing ◽  
Impaired Wound Healing ◽  
Environmental Signals ◽  
Aryl Hydrocarbon ◽  
Normal Wound Healing ◽  
Full Benefit ◽  
The Difference

Aryl hydrocarbon receptor nuclear translocator (ARNT) is a transcription factor that binds to partners to mediate responses to environmental signals. To investigate its role in the innate immune system, floxed ARNT mice were bred with lysozyme M-Cre recombinase animals to generate lysozyme M-ARNT (LAR) mice with reduced ARNT expression. Myeloid cells of LAR mice had altered mRNA expression and delayed wound healing. Interestingly, when the animals were rendered diabetic, the difference in wound healing between the LAR mice and their littermate controls was no longer present, suggesting that decreased myeloid cell ARNT function may be an important factor in impaired wound healing in diabetes. Deferoxamine (DFO) improves wound healing by increasing hypoxia-inducible factors, which require ARNT for function. DFO was not effective in wounds of LAR mice, again suggesting that myeloid cells are important for normal wound healing and for the full benefit of DFO. These findings suggest that myeloid ARNT is important for immune function and wound healing. Increasing ARNT and, more specifically, myeloid ARNT may be a therapeutic strategy to improve wound healing.

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