Orthogonal arrays of particles in plasma membranes of the gastric parietal cell

1978 ◽  
Vol 192 (2) ◽  
pp. 297-303 ◽  
Author(s):  
Cesare Bordi ◽  
Alain Perrelet
Keyword(s):  
Parietal Cell ◽  
Plasma Membranes ◽  
Orthogonal Arrays ◽  
Gastric Parietal Cell ◽  
Orthogonal Arrays Of Particles

scholarly journals Aquaporin-4 Dynamics in Orthogonal Arrays in Live Cells Visualized by Quantum Dot Single Particle Tracking

2008 ◽  
Vol 19 (8) ◽  
pp. 3369-3378 ◽  
Author(s):  
Jonathan M. Crane ◽  
Alfred N. Van Hoek ◽  
William R. Skach ◽  
A. S. Verkman
Keyword(s):  
Plasma Membranes ◽  
Freeze Fracture ◽  
Orthogonal Arrays ◽  
Cell Types ◽  
Single Particle Tracking ◽  
Aquaporin 4 ◽  
Live Cells ◽  
Restoring Force ◽  
Orthogonal Arrays Of Particles ◽  
Freeze Fracture Electron Microscopy

Freeze-fracture electron microscopy (FFEM) indicates that aquaporin-4 (AQP4) water channels can assemble in cell plasma membranes in orthogonal arrays of particles (OAPs). We investigated the determinants and dynamics of AQP4 assembly in OAPs by tracking single AQP4 molecules labeled with quantum dots at an engineered external epitope. In several transfected cell types, including primary astrocyte cultures, the long N-terminal “M1” form of AQP4 diffused freely, with diffusion coefficient ∼5 × 10−10 cm2/s, covering ∼5 μm in 5 min. The short N-terminal “M23” form of AQP4, which by FFEM was found to form OAPs, was relatively immobile, moving only ∼0.4 μm in 5 min. Actin modulation by latrunculin or jasplakinolide did not affect AQP4-M23 diffusion, but deletion of its C-terminal postsynaptic density 95/disc-large/zona occludens (PDZ) binding domain increased its range by approximately twofold over minutes. Biophysical analysis of short-range AQP4-M23 diffusion within OAPs indicated a spring-like potential, with a restoring force of ∼6.5 pN/μm. These and additional experiments indicated that 1) AQP4-M1 and AQP4-M23 isoforms do not coassociate in OAPs; 2) OAPs can be imaged directly by total internal reflection fluorescence microscopy; and 3) OAPs are relatively fixed, noninterconvertible assemblies that do not require cytoskeletal or PDZ-mediated interactions for formation. Our measurements are the first to visualize OAPs in live cells.

Fatty Acids in a High-Fat Diet Potentially Induce Gastric Parietal-Cell Damage and Metaplasia in Mice

2017 ◽  
Vol 152 (5) ◽  
pp. S418
Author(s):  
Yuki Hirata ◽  
Shinji Fukuda ◽  
Kazuhiko Yamada ◽  
Kazuhide Higuchi ◽  
Yuki I. Kawamura ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Parietal Cell ◽  
High Fat Diet ◽  
Cell Damage ◽  
High Fat ◽  
Gastric Parietal Cell

scholarly journals Anemia and hematinic deficiencies in gastric parietal cell antibody-positive and -negative oral mucosal disease patients with microcytosis

2017 ◽  
Vol 116 (8) ◽  
pp. 613-619 ◽  
Author(s):  
Hung-Pin Lin ◽  
Yu-Hsueh Wu ◽  
Yi-Ping Wang ◽  
Yang-Che Wu ◽  
Julia Yu-Fong Chang ◽  
...  
Keyword(s):  
Parietal Cell ◽  
Gastric Parietal Cell ◽  
Mucosal Disease ◽  
Cell Antibody ◽  
Parietal Cell Antibody

3-Dimensional Modeling of the Gastric Parietal Cell

2002 ◽  
pp. 295-304
Author(s):  
John G. Forte ◽  
Joseph G. Duman ◽  
Nimesh J. Pathak ◽  
Kent L. Mcdonald
Keyword(s):  
Parietal Cell ◽  
Gastric Parietal Cell ◽  
3 Dimensional ◽  
Dimensional Modeling

Gastric parietal cell development: Expression of the H+/K+ATPase subunits coincides with the biogenesis of the secretory membranes

1993 ◽  
Vol 71 (3) ◽  
pp. 191-200 ◽  
Author(s):  
JOHN M. PETTITT ◽  
BAN-HOCK TOH ◽  
JUDY M. CALLAGHAN ◽  
PAUL A. GLEESON ◽  
IAN R. VAN DRIEL
Keyword(s):  
Parietal Cell ◽  
Cell Development ◽  
Gastric Parietal Cell ◽  
Atpase Subunits
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