Orthogonal arrays of particles in the gastric parietal cell of the rat: Differences between superficial and basal cells in the gland and after pentagastrin or metiamide treatment

1986 ◽  
Vol 215 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Cesare Bordi ◽  
Myl�gne Amherdt ◽  
Alain Perrelet
Keyword(s):  
Parietal Cell ◽  
Orthogonal Arrays ◽  
Basal Cells ◽  
Gastric Parietal Cell ◽  
Orthogonal Arrays Of Particles

Fatty Acids in a High-Fat Diet Potentially Induce Gastric Parietal-Cell Damage and Metaplasia in Mice

2017 ◽  
Vol 152 (5) ◽  
pp. S418
Author(s):  
Yuki Hirata ◽  
Shinji Fukuda ◽  
Kazuhiko Yamada ◽  
Kazuhide Higuchi ◽  
Yuki I. Kawamura ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Parietal Cell ◽  
High Fat Diet ◽  
Cell Damage ◽  
High Fat ◽  
Gastric Parietal Cell

scholarly journals Anemia and hematinic deficiencies in gastric parietal cell antibody-positive and -negative oral mucosal disease patients with microcytosis

2017 ◽  
Vol 116 (8) ◽  
pp. 613-619 ◽  
Author(s):  
Hung-Pin Lin ◽  
Yu-Hsueh Wu ◽  
Yi-Ping Wang ◽  
Yang-Che Wu ◽  
Julia Yu-Fong Chang ◽  
...  
Keyword(s):  
Parietal Cell ◽  
Gastric Parietal Cell ◽  
Mucosal Disease ◽  
Cell Antibody ◽  
Parietal Cell Antibody

3-Dimensional Modeling of the Gastric Parietal Cell

2002 ◽  
pp. 295-304
Author(s):  
John G. Forte ◽  
Joseph G. Duman ◽  
Nimesh J. Pathak ◽  
Kent L. Mcdonald
Keyword(s):  
Parietal Cell ◽  
Gastric Parietal Cell ◽  
3 Dimensional ◽  
Dimensional Modeling

Gastric parietal cell development: Expression of the H+/K+ATPase subunits coincides with the biogenesis of the secretory membranes

1993 ◽  
Vol 71 (3) ◽  
pp. 191-200 ◽  
Author(s):  
JOHN M. PETTITT ◽  
BAN-HOCK TOH ◽  
JUDY M. CALLAGHAN ◽  
PAUL A. GLEESON ◽  
IAN R. VAN DRIEL
Keyword(s):  
Parietal Cell ◽  
Cell Development ◽  
Gastric Parietal Cell ◽  
Atpase Subunits

scholarly journals Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells

2001 ◽  
Vol 154 (6) ◽  
pp. 1235-1244 ◽  
Author(s):  
Monica Carmosino ◽  
Giuseppe Procino ◽  
Grazia Paola Nicchia ◽  
Roberta Mannucci ◽  
Jean-Marc Verbavatz ◽  
...  
Keyword(s):  
Cell Line ◽  
Basolateral Membrane ◽  
Water Channel ◽  
Freeze Fracture ◽  
Orthogonal Arrays ◽  
Short Term ◽  
Water Permeability Coefficient ◽  
Cell Surface Biotinylation ◽  
Osmotic Water ◽  
Orthogonal Arrays Of Particles

To test the involvement of the water channel aquaporin (AQP)-4 in gastric acid physiology, the human gastric cell line (HGT)-1 was stably transfected with rat AQP4. AQP4 was immunolocalized to the basolateral membrane of transfected HGT-1 cells, like in native parietal cells. Expression of AQP4 in transfected cells increased the osmotic water permeability coefficient (Pf) from 2.02 ± 0.3 × 10−4 to 16.37 ± 0.5 × 10−4 cm/s at 20°C. Freeze-fracture EM showed distinct orthogonal arrays of particles (OAPs), the morphological signature of AQP4, on the plasma membrane of AQP4-expressing cells. Quantitative morphometry showed that the density of OAPs was 2.5 ± 0.3% under basal condition and decreased by 50% to 1.2 ± 0.3% after 20 min of histamine stimulation, mainly due to a significant decrease of the OAPs number. Concomitantly, Pf decreased by ∼35% in 20-min histamine-stimulated cells. Both Pf and OAPs density were not modified after 10 min of histamine exposure, time at which the maximal hormonal response is observed. Cell surface biotinylation experiments confirmed that AQP4 is internalized after 20 min of histamine exposure, which may account for the downregulation of water transport. This is the first evidence for short term rearrangement of OAPs in an established AQP4-expressing cell line.

Absence of orthogonal arrays in kidney, brain and muscle from transgenic knockout mice lacking water channel aquaporin-4

1997 ◽  
Vol 110 (22) ◽  
pp. 2855-2860 ◽  
Author(s):  
J.M. Verbavatz ◽  
T. Ma ◽  
R. Gobin ◽  
A.S. Verkman
Keyword(s):  
Cho Cells ◽  
Collecting Duct ◽  
Water Channel ◽  
Freeze Fracture ◽  
Orthogonal Arrays ◽  
Knock Out ◽  
Orthogonal Arrays Of Particles ◽  
Freeze Fracture Electron Microscopy ◽  
Knock Out Mice ◽  
And Function

Freeze-fracture electron microscopy (FFEM) of kidney collecting duct, muscle, astrocytes in brain, and other mammalian tissues has revealed regular square arrays of intramembrane particles called orthogonal arrays of particles (OAPs). Their possible role in membrane structure and transport have been proposed, and their absence or decrease has been noted in a variety of hereditary and acquired diseases. A transgenic mouse lacking water channel AQP4 was used to show that AQP4 is the OAP protein. FFEM was done on kidney, skeletal muscle, and brain from AQP4 wild-type [+/+], heterozygous [+/−] and knock-out [-/-] mice. The [-/-] mice did not express detectable AQP4 protein, but were grossly indistinguishable from [+/+] mice. FFEM was done on blinded samples of kidney, brain and muscle from 9 mice. In all 6 kidney samples from [+/+] and [+/−] mice, OAPs similar to those in AQP4-transfected CHO cells were found in basolateral membranes of collecting duct principal cells. In all muscle and brain samples from [+/+] and [+/−] mice, OAPs of identical ultrastructure to those in kidney were seen, but in smaller patch sizes. OAPs were not seen in any sample from [-/-] mice. Label-fracture analysis using a peptide-derived AQP4 polyclonal antibody showed immunogold labeling of OAPs in AQP4-expressing CHO cells. These studies provide direct evidence that AQP4 is required for formation of OAPs and is a component of OAPs, thus establishing the identity and function of OAPs.

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