Orthogonal arrays of particles in plasma membranes of Müller cells in the guinea pig retina

Glia ◽  
1989 ◽  
Vol 2 (4) ◽  
pp. 273-285 ◽  
Author(s):  
Takahiro Gotow ◽  
Paulo H. Hashimoto
Keyword(s):  
Guinea Pig ◽  
Plasma Membranes ◽  
Müller Cells ◽  
Orthogonal Arrays ◽  
Muller Cells ◽  
Orthogonal Arrays Of Particles

Outwardly rectifying K+ channels display clustering in guinea pig retinal Müller cells

1999 ◽  
Vol 276 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Thomas Pannicke ◽  
Andreas Reichenbach ◽  
Winfried Reichelt
Keyword(s):  
Guinea Pig ◽  
Müller Cells ◽  
Muller Cells ◽  
K Channels

Patch-clamp recordings from Müller (glial) cell endfeet in the intact isolated retina and acutely isolated Müller cells of mouse and guinea-pig

Neuroscience ◽  
1993 ◽  
Vol 57 (3) ◽  
pp. 599-613 ◽  
Author(s):  
W. Reichelt ◽  
T. Müller ◽  
A. Pastor ◽  
T. Pannicke ◽  
P.M. Orkand ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Guinea Pig ◽  
Glial Cell ◽  
Müller Cells ◽  
Muller Cells ◽  
Müller Glial Cell ◽  
Isolated Retina

scholarly journals Aquaporin-4 Dynamics in Orthogonal Arrays in Live Cells Visualized by Quantum Dot Single Particle Tracking

2008 ◽  
Vol 19 (8) ◽  
pp. 3369-3378 ◽  
Author(s):  
Jonathan M. Crane ◽  
Alfred N. Van Hoek ◽  
William R. Skach ◽  
A. S. Verkman
Keyword(s):  
Plasma Membranes ◽  
Freeze Fracture ◽  
Orthogonal Arrays ◽  
Cell Types ◽  
Single Particle Tracking ◽  
Aquaporin 4 ◽  
Live Cells ◽  
Restoring Force ◽  
Orthogonal Arrays Of Particles ◽  
Freeze Fracture Electron Microscopy

Freeze-fracture electron microscopy (FFEM) indicates that aquaporin-4 (AQP4) water channels can assemble in cell plasma membranes in orthogonal arrays of particles (OAPs). We investigated the determinants and dynamics of AQP4 assembly in OAPs by tracking single AQP4 molecules labeled with quantum dots at an engineered external epitope. In several transfected cell types, including primary astrocyte cultures, the long N-terminal “M1” form of AQP4 diffused freely, with diffusion coefficient ∼5 × 10−10 cm2/s, covering ∼5 μm in 5 min. The short N-terminal “M23” form of AQP4, which by FFEM was found to form OAPs, was relatively immobile, moving only ∼0.4 μm in 5 min. Actin modulation by latrunculin or jasplakinolide did not affect AQP4-M23 diffusion, but deletion of its C-terminal postsynaptic density 95/disc-large/zona occludens (PDZ) binding domain increased its range by approximately twofold over minutes. Biophysical analysis of short-range AQP4-M23 diffusion within OAPs indicated a spring-like potential, with a restoring force of ∼6.5 pN/μm. These and additional experiments indicated that 1) AQP4-M1 and AQP4-M23 isoforms do not coassociate in OAPs; 2) OAPs can be imaged directly by total internal reflection fluorescence microscopy; and 3) OAPs are relatively fixed, noninterconvertible assemblies that do not require cytoskeletal or PDZ-mediated interactions for formation. Our measurements are the first to visualize OAPs in live cells.

K+ Channels of Müller Glial Cells in Retinal Disorders

2018 ◽  
Vol 17 (4) ◽  
pp. 255-260 ◽  
Author(s):  
Feng Gao ◽  
Lin-Jie Xu ◽  
Yuan Zhao ◽  
Xing-Huai Sun ◽  
Zhongfeng Wang
Keyword(s):  
Müller Cells ◽  
Major Type ◽  
Delayed Rectifier ◽  
Muller Cells ◽  
Bkca Channels ◽  
K Channels ◽  
Functional Changes ◽  
Physiological Properties ◽  
Retinal Disorders ◽  
Inwardly Rectifying

Background & Objective: Müller cell is the major type of glial cell in the vertebrate retina. Müller cells express various types of K+ channels, such as inwardly rectifying K+ (Kir) channels, big conductance Ca2+-activated K+ (BKCa) channels, delayed rectifier K+ channels (KDR), and transient A-type K+ channels. These K+ channels play important roles in maintaining physiological functions of Müller cells. Under some retinal pathological conditions, the changed expression and functions of K+ channels may contribute to retinal pathogenesis. Conclusion: In this article, we reviewed the physiological properties of K+ channels in retinal Müller cells and the functional changes of these channels in retinal disorders.

scholarly journals Melatonin attenuates oxidative stress and inflammation of Müller cells in diabetic retinopathy via activating the Sirt1 pathway

2021 ◽  
Vol 137 ◽  
pp. 111274
Author(s):  
Yuanyuan Tu ◽  
E Song ◽  
Zhenzhen Wang ◽  
Na Ji ◽  
Linling Zhu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Retinopathy ◽  
Müller Cells ◽  
Muller Cells

Vitreous from idiopathic epiretinal membrane patients induces glial-to-mesenchymal transition in Müller cells

2021 ◽  
pp. 166181
Author(s):  
Adwaid Manu Krishna Chandran ◽  
Daniela Coltrini ◽  
Mirella Belleri ◽  
Sara Rezzola ◽  
Elena Gambicorti ◽  
...  
Keyword(s):  
Epiretinal Membrane ◽  
Müller Cells ◽  
Muller Cells ◽  
Mesenchymal Transition ◽  
Idiopathic Epiretinal Membrane
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