Rational Design and Development of Near-Infrared-Emitting Firefly Luciferins Available In Vivo

2012 ◽  
Vol 52 (4) ◽  
pp. 1175-1179 ◽  
Author(s):  
Ryosuke Kojima ◽  
Hideo Takakura ◽  
Takeaki Ozawa ◽  
Yukio Tada ◽  
Tetsuo Nagano ◽  
...  
Keyword(s):  
Rational Design ◽  
Near Infrared ◽  
Design And Development

Rational Design and Development of Near-Infrared-Emitting Firefly Luciferins Available In Vivo

2012 ◽  
Vol 125 (4) ◽  
pp. 1213-1217 ◽  
Author(s):  
Ryosuke Kojima ◽  
Hideo Takakura ◽  
Takeaki Ozawa ◽  
Yukio Tada ◽  
Tetsuo Nagano ◽  
...  
Keyword(s):  
Rational Design ◽  
Near Infrared ◽  
Design And Development

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

scholarly journals Rational design of a NIR-II fluorescent nano-system with maximized fluorescence performance and applications

2021 ◽  
Author(s):  
Haoli Yu ◽  
Yuesong Wang ◽  
Yan Chen ◽  
Min Ji
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Nir Fluorescence

Purpose: Near-infrared (NIR) fluorescence imaging (FI) become a research hotspot in the field of in vivo imaging. Here, we intend to synthesize a NIR-II fluorescence nano-system with an excellent fluorescence...

Rational design of a lysosome-targeting and near-infrared absorbing Ru(ii)–BODIPY conjugate for photodynamic therapy

2021 ◽  
Vol 57 (14) ◽  
pp. 1790-1793
Author(s):  
Liping Qiao ◽  
Jiangping Liu ◽  
Yunhong Han ◽  
Fangmian Wei ◽  
Xinxing Liao ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Malignant Melanoma ◽  
Rational Design ◽  
Near Infrared ◽  
Inhibitory Efficiency ◽  
A375 Cells

A lysosome-targeting and near-infrared absorbing Ru(ii)–BODIPY conjugate exhibits prominent tumor inhibitory efficiency in malignant melanoma A375 cells and in vivo experiments.

scholarly journals Enhanced Photothermal and Photoacoustic Performance of Graphene Oxide in NIR-II Biowindow by Chemical Reduction

Photonics ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 2
Author(s):  
Xiaoye Su ◽  
Liantong Li ◽  
Dandan Cui ◽  
Wei Fang ◽  
Yujiao Shi
Keyword(s):  
Graphene Oxide ◽  
Rational Design ◽  
Near Infrared ◽  
Chemical Reduction ◽  
Black Body ◽  
Absorption Enhancement ◽  
Chemically Reduced Graphene Oxide ◽  
Novel Strategy

We report on a novel strategy for constructing graphene oxide nanomaterials with strongly enhanced photothermal (PT) and photoacoustic (PA) performance in the near-infrared (NIR)-II biowindow by chemical reduction. Optical spectra clearly reveal that obvious enhancement of optical absorption is observed in the whole NIR wideband from the NIR-I to NIR-II region for chemically reduced graphene oxide (CR-G) nanomaterials, which is mainly arising from the restoration of the electronic conjugation within the graphene oxide sheets and therefore inducing a black-body re-introduction effect of typical graphite-like materials. We experimentally synthesized CR-G samples with different degrees of reduction to demonstrate the efficiency of the proposed strategy. Experimental results show that the PT performance of the CR-G samples is greatly improved owing to the absorption enhancement by chemical reduction in the NIR-II biowindow. Furthermore, both in vitro and in vivo PA imaging of the CR-G samples with different degrees of reduction are performed to demonstrate their enhanced NIR-II PA performances. This work provides a feasible guidance for the rational design of graphene oxide nanomaterials with great potential for PT and PA applications in the NIR-II biowindow by chemical reduction.

Rational Design of Ratiometric Near-Infrared Aza-BODIPY-Based Fluorescent Probe for in Vivo Imaging of Endogenous Hydrogen Peroxide

2019 ◽  
Vol 3 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Wenle Mao ◽  
Mingming Zhu ◽  
Chenxu Yan ◽  
Yiyu Ma ◽  
Zhiqian Guo ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared

Rational design of a novel mitochondrial-targeted near-infrared fluorescent pH probe for imaging in living cells and in vivo

RSC Advances ◽  
2016 ◽  
Vol 6 (98) ◽  
pp. 95708-95714 ◽  
Author(s):  
Peng Wang ◽  
Jinxin Huang ◽  
Yueqing Gu
Keyword(s):  
Rational Design ◽  
Near Infrared ◽  
Living Cells ◽  
Ph Changes ◽  
Ph Probe

A novel mitochondrial-targeted fluorescent pH probe NIR-F1 could sensitively and selectively monitor pH changes in living cells and living mice.

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