Rational design of a novel mitochondrial-targeted near-infrared fluorescent pH probe for imaging in living cells and in vivo

RSC Advances ◽  
2016 ◽  
Vol 6 (98) ◽  
pp. 95708-95714 ◽  
Author(s):  
Peng Wang ◽  
Jinxin Huang ◽  
Yueqing Gu
Keyword(s):  
Rational Design ◽  
Near Infrared ◽  
Living Cells ◽  
Ph Changes ◽  
Ph Probe

A novel mitochondrial-targeted fluorescent pH probe NIR-F1 could sensitively and selectively monitor pH changes in living cells and living mice.

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

A near-infrared fluorescence off–on probe for sensitive imaging of hydrogen polysulfides in living cells and mice in vivo

2017 ◽  
Vol 53 (62) ◽  
pp. 8759-8762 ◽  
Author(s):  
Yu Fang ◽  
Wei Chen ◽  
Wen Shi ◽  
Hongyu Li ◽  
Ming Xian ◽  
...  
Keyword(s):  
Near Infrared ◽  
Living Cells ◽  
Near Infrared Fluorescence

A new near-infrared fluorescence off–on probe with phenyl 2-(benzoylthio)benzoate as the recognition moiety is developed and applied in imaging H2Sn in living cells and mice in vivo.

scholarly journals A near-infrared phosphorescent iridium(iii) complex for imaging of cysteine and homocysteine in living cells and in vivo

RSC Advances ◽  
2017 ◽  
Vol 7 (83) ◽  
pp. 52621-52625 ◽  
Author(s):  
Yongquan Wu ◽  
Renmiao Wu ◽  
Huifang Li ◽  
Hong Zeng ◽  
Yuanyan Li ◽  
...  
Keyword(s):  
Near Infrared ◽  
Living Cells

A novel NIR-emitting iridium(iii) complex was developed to detect Cys/Hcy levels in vitro and in vivo.

High-Quantum-Yield Mitochondria-Targeting Near-Infrared Fluorescent Probe for Imaging Native Hypobromous Acid in Living Cells and in Vivo

2017 ◽  
Vol 89 (3) ◽  
pp. 1787-1792 ◽  
Author(s):  
Xiaojun Liu ◽  
Aishan Zheng ◽  
Dongrui Luan ◽  
Xiaoting Wang ◽  
Fanpeng Kong ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
High Quantum Yield ◽  
High Quantum ◽  
Hypobromous Acid ◽  
Mitochondria Targeting

scholarly journals Rational design of a NIR-II fluorescent nano-system with maximized fluorescence performance and applications

2021 ◽  
Author(s):  
Haoli Yu ◽  
Yuesong Wang ◽  
Yan Chen ◽  
Min Ji
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Nir Fluorescence

Purpose: Near-infrared (NIR) fluorescence imaging (FI) become a research hotspot in the field of in vivo imaging. Here, we intend to synthesize a NIR-II fluorescence nano-system with an excellent fluorescence...

scholarly journals Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe

2018 ◽  
Vol 6 (41) ◽  
pp. 6637-6645 ◽  
Author(s):  
Min Gao ◽  
Rui Wang ◽  
Fabiao Yu ◽  
Bowei Li ◽  
Lingxin Chen
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Hypoxic Stress ◽  
Multicellular Spheroid ◽  
Sulfane Sulfur

Imaging of sulfane sulfur expression changes in living cells, in 3D-multicellular spheroid, in hippocampus and in vivo under hypoxic stress via a selenium-containing near-infrared fluorescent probe.

A novel near-infrared xanthene-based fluorescent probe for detection of thiophenol in vitro and in vivo

2020 ◽  
Vol 44 (40) ◽  
pp. 17360-17367
Author(s):  
Yongquan Wu ◽  
Aiping Shi ◽  
Huiying Liu ◽  
Yuanyan Li ◽  
Weican Lun ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells

A novel near-infrared xanthene-based fluorescent probe for detection of thiophenol in living cells and mice.

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