Rational Design of Near-Infrared Cyanine-Based Fluorescent Probes for Rapid In Vivo Sensing Cysteine

2020 ◽  
Author(s):  
Hehe Zhang ◽  
Chenxu Yan ◽  
Hui Li ◽  
Lei Shi ◽  
Ruofei Wang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Rational Design ◽  
Near Infrared ◽  
In Vivo Sensing

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

scholarly journals Cypate and Cypate-Glucosamine as Near-Infrared Fluorescent Probes for In Vivo Tumor Imaging

2019 ◽  
Vol 95 (5) ◽  
pp. 475-489
Author(s):  
Mona Doshi ◽  
Daniel A. Nierenberg ◽  
Orielyz Flores-Fernandez ◽  
Pragney Deme ◽  
Edilu Becerra ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Tumor Imaging

Rational design of fluorescent probes for targeted in vivo nitroreductase visualization

2020 ◽  
Vol 18 (25) ◽  
pp. 4744-4747 ◽  
Author(s):  
Jie Gao ◽  
Xiaofan Yin ◽  
Mimi Li ◽  
Ji-An Chen ◽  
Jiahui Tan ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Rational Design

Three fluorescent probes were made by conjugation of para-, ortho-, and meta-nitrobenzene to the BODIPY core via a thiolether bond. It revealed that the linkage and nitro substituent position significantly influence the capability of NTR detection.

Synthesis and evaluation of curcumin-based near-infrared fluorescent probes for the in vivo optical imaging of amyloid-β plaques

2021 ◽  
pp. 105167
Author(s):  
Yong Dae Park ◽  
Mayank Kinger ◽  
Changho Min ◽  
Sang Yeob Lee ◽  
Youngjoo Byun ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Amyloid Β ◽  
In Vivo Optical Imaging

Amyloid-β Deposits Target Efficient Near-Infrared Fluorescent Probes: Synthesis, in Vitro Evaluation, and in Vivo Imaging

2016 ◽  
Vol 88 (3) ◽  
pp. 1944-1950 ◽  
Author(s):  
Hualong Fu ◽  
Peiyu Tu ◽  
Liu Zhao ◽  
Jiapei Dai ◽  
Boli Liu ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Amyloid Β ◽  
In Vitro Evaluation

scholarly journals Activatable Organic Near-Infrared Fluorescent Probes Based on a Bacteriochlorin Platform: Synthesis and Multicolor in Vivo Imaging with a Single Excitation

2014 ◽  
Vol 25 (2) ◽  
pp. 362-369 ◽  
Author(s):  
Toshiko Harada ◽  
Kohei Sano ◽  
Kazuhide Sato ◽  
Rira Watanabe ◽  
Zhanqian Yu ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
In Vivo Imaging ◽  
Near Infrared

scholarly journals Rational design of a NIR-II fluorescent nano-system with maximized fluorescence performance and applications

2021 ◽  
Author(s):  
Haoli Yu ◽  
Yuesong Wang ◽  
Yan Chen ◽  
Min Ji
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Nir Fluorescence

Purpose: Near-infrared (NIR) fluorescence imaging (FI) become a research hotspot in the field of in vivo imaging. Here, we intend to synthesize a NIR-II fluorescence nano-system with an excellent fluorescence...

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