Rational Design of Ratiometric Near-Infrared Aza-BODIPY-Based Fluorescent Probe for in Vivo Imaging of Endogenous Hydrogen Peroxide

2019 ◽  
Vol 3 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Wenle Mao ◽  
Mingming Zhu ◽  
Chenxu Yan ◽  
Yiyu Ma ◽  
Zhiqian Guo ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared

A near-infrared ratiometric/turn-on fluorescent probe for in vivo imaging of hydrogen peroxide in a murine model of acute inflammation

2018 ◽  
Vol 1024 ◽  
pp. 169-176 ◽  
Author(s):  
Jingli Hou ◽  
Meng Qian ◽  
Huanhuan Zhao ◽  
Yingchun Li ◽  
Yongfang Liao ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Murine Model ◽  
In Vivo Imaging ◽  
Acute Inflammation ◽  
Near Infrared ◽  
Turn On

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

A novel colorimetric and near-infrared fluorescent probe for hydrogen peroxide imaging in vitro and in vivo

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85957-85963 ◽  
Author(s):  
Peng Wang ◽  
Ke Wang ◽  
Dan Chen ◽  
Yibo Mao ◽  
Yueqing Gu
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Near Infrared

A novel NIR fluorescent probe (DCM-B2) based on dicyanomethylene-4H-pyran was synthesized for the detection of H2O2.

A hypoxia-activated near infrared fluorescent probe for cyclooxygenase-2 and in vivo imaging for tumor and inflammation

2018 ◽  
Vol 265 ◽  
pp. 582-590 ◽  
Author(s):  
Yuezheng Ti ◽  
Ling Yu ◽  
Yao Tang ◽  
Tongxia Jin ◽  
Ming Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Cyclooxygenase 2

A near-infrared fluorescent probe based on the hemicyanine skeleton for the detection of hydrogen peroxide in vivo

2022 ◽  
Vol 266 ◽  
pp. 120435
Author(s):  
Sheng Wang ◽  
Yu Zhang ◽  
Tian-Ran Wang ◽  
Yu-Jun Liu ◽  
Shi-Li Shen ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Near Infrared

Design, synthesis and application of a near-infrared fluorescent probe for in vivo imaging of aminopeptidase N

2017 ◽  
Vol 53 (68) ◽  
pp. 9438-9441 ◽  
Author(s):  
Xinyuan He ◽  
Yiming Hu ◽  
Wen Shi ◽  
Xiaohua Li ◽  
Huimin Ma
Keyword(s):  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Aminopeptidase N ◽  
Design Synthesis ◽  
First Time

We have, for the first time, developed a near-infrared fluorescent probe for aminopeptidase N by combining a hemicyanine and an alanyl residue.

A Selective Near-Infrared Fluorescent Probe for In Vivo Imaging of Thiophenols from a Focused Library

2016 ◽  
Vol 11 (24) ◽  
pp. 3575-3582 ◽  
Author(s):  
Yue Pan ◽  
Tian-Bing Ren ◽  
Dan Cheng ◽  
Ze-Bing Zeng ◽  
Lin Yuan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Near Infrared

Nanoparticles based on quantum dots and a luminol derivative: implications for in vivo imaging of hydrogen peroxide by chemiluminescence resonance energy transfer

2016 ◽  
Vol 52 (22) ◽  
pp. 4132-4135 ◽  
Author(s):  
Eun Sook Lee ◽  
V. G. Deepagan ◽  
Dong Gil You ◽  
Jueun Jeon ◽  
Gi-Ra Yi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Energy Transfer ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Hybrid Nanoparticles ◽  
Infrared Wavelength ◽  
Luminol Derivative

Hybrid nanoparticles allow for imaging hydrogen peroxide via chemiluminescence resonance energy transfer in the near-infrared wavelength range.

scholarly journals Rational design of a NIR-II fluorescent nano-system with maximized fluorescence performance and applications

2021 ◽  
Author(s):  
Haoli Yu ◽  
Yuesong Wang ◽  
Yan Chen ◽  
Min Ji
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Nir Fluorescence

Purpose: Near-infrared (NIR) fluorescence imaging (FI) become a research hotspot in the field of in vivo imaging. Here, we intend to synthesize a NIR-II fluorescence nano-system with an excellent fluorescence...

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