Influence of ferrocene and its derivatives on growth of Escherichia coli (ATTC 25922)

This study is a continued investigation of the influence of ferrocene and its derivatives on trypsin activity. The goal was to examine the effect in vivo, by monitoring the growth of the bacteria Escherichia coli. The growth of the bacteria with the addition of ferrocene and derivatives of various concentrations was followed up spectrophotometrically, measuring changes in OD, correlating OD with the number of formed bacterial colonies and comparing the results as the mean generation time. The obtained results in relation to control experiments indicate a very strong inhibitory action of ferrocene and (dimethylaminomethyl) ferrocene, a medium or modest inhibitory effect of methyl 1'-acetamidoferrocene- 1-carboxylate and benzyl 1'-methoxycarbonyl-1-ferrocenecarbamate; influence of benzyl 1'-carboxy-1-ferrocenecarbamate is negligible, while 1'-acetamidoferrocene-1-carboxylic acid causes the increase in the growth of Escherichia coli.