Time-Interval Emphasis in an Aeronautical Dual-Task Context: A Countermeasure to Task Absorption

Objective: We tested a training method intended to prevent unsafe aeronautical behavior (i.e., too much time spent gazing inside the cockpit) induced by the modern cockpit, by teaching individuals to perform a task complementing the see-and-avoid mandatory safety task within a limited time interval. Background: Aeronautical activities led crews to perform several tasks simultaneously in an ergonomic environment under constant change. See and avoid remains one of the main safety tasks during visual flight. However, modern cockpits induce absorption and impair performance of this safety task. Many laboratory studies showed the relevance of training methods for managing dual-task situations and estimating time intervals. Method: A specific virtual environment was developed to expose participants to a dual-task situation in which time-interval emphasis was provided in real time. Two types of emphasis training were tested: a permissive one that allowed participants to pursue the inside-cockpit task beyond the time limit and a nonpermissive one that did not. Results: The best time-interval acquisition, with retention up to 24 hr later, was observed in the nonpermissive condition, but task performances immediately after the training sessions were equivalent across conditions. Conclusion: Time-emphasis training appears to be an efficient means of promoting absorption resistance while preserving task performance. Transferability of time-interval estimation skills has yet to be tested. Application: Most areas of application for absorption resistance (aviation, shipping, rail, road, etc.) could benefit from this type of training to manage multitask situations.

Characterization of a Novel Gene, wosA, Regulating FlhDC Expression in Proteus mirabilis

ABSTRACT In this study, we describe wosA, a Proteus mirabilis gene identified by its ability to increase swarming motility when overexpressed. At various times during the swarming cycle, the increased expression of wosA resulted in a 4- to 16-fold upregulation of the transcription of flhDC, encoding the master regulator of the flagellar cascade. In turn, the expression of flaA, encoding flagellin, was substantially increased in wosA-overexpressing strains. The overexpression of wosA also resulted in constitutive swarmer cell differentiation in liquid medium, a normally nonpermissive condition. However, in wosA-overexpressing strains, the onset of swarming was not altered. A null wosA allele resulted in a slight decrease in swarming motility. The expression of wosA was growth phase dependent during growth in liquid and on agar plates during swarmer cell differentiation. Increasing the viscosity of liquid medium by the addition of polyvinylpyrrolidone induced swarmer cell differentiation and resulted in a fourfold increase in wosA transcription. A fliL mutation that results in constitutive swarmer cell elongation also increased wosA transcription. In this study, we discuss the possible role of the wosA gene product in signal transduction from solid surfaces to induce swarmer cell differentiation, possibly via alterations in the motor switch complex. This study also suggests that despite constitutive swarmer cell differentiation in wosA-overexpressing strains, there are additional regulatory and/or environmental conditions that may control the onset of swarming migration.

Giant axonal neuropathy: a conditional mutation affecting cytoskeletal organization.

Giant axonal neuropathy (GAN) results from autosomal recessive mutations (gan-) that affect cytoskeletal organization; specifically, intermediate filaments (IFs) are found collapsed into massive bundles in a variety of different cell types. We studied the gan- fibroblast lines WG321 and WG139 derived from different GAN patients. Although previous studies implied that the gan- IF phenotype was constitutive, we find that it is conditional. That is, when cells were grown under the permissive condition of medium containing over 2% fetal calf serum, most cells had normal IF organization. IF bundles formed when gan- cells were transferred to the nonpermissive condition of low (0.1%) serum. Microtubule organization appeared normal in the presence or absence of serum. The effect of serum starvation was largely blocked or reversed by the addition of BSA to the culture media. We found no evidence that the gan- phenotype depends upon progress through the cell cycle. We discuss the possible role of serum effects in the etiology of GAN and speculate as to the molecular nature of the gan- defect.