Status of springs in mountain watershed of western Nepal

The study, conducted in western hilly areas of Nepal, inventoried and mapped over 4,222 springs from five different watersheds. The study showed that more than 50% of the spring sources were found under natural conditions, i.e., open spring whereas 15% of them were of pond type. Similarly, the other 15% spring was recorded as a concrete structure or tank while 1% was determined to be a well. Attempts were made to identify if a change in water discharge from springs relates to rainfall patterns. The inter-annual variability analysis shows a significant fluctuation suggesting variation in water discharge across spring sources. The lowest amount of yearly rainfall received in the river basin is governed by decreasing water flow from the springs in the upper and mid-hills of Nepal. Besides, the intra-annual variation (i.e., seasonal and concentrative nature of rainfall only during monsoon) leads to shortage of drinking water and other domestic purposes (e.g., cooking, cleaning) during the dry months of the year. This study, based on the estimation of discharge flow in these springs, revealed that about 70% were decreasing and, in particular, the flow over the recent ten years decreased significantly.

Bacteriological Assessment and Antibiotics Susceptibility Profile of Bacteria Recovered from Pond Water, Fish Skin, and Gut in Ile-Ife, Osun State, Nigeria

Fish currently provide 6.7% of all protein consumed by humans globally, nevertheless, aquaculture system has been linked to fish and environmental contamination and disease outbreak. This study aims to isolate, identify, and characterise, bacteria in fish and pond water as well as the antibiotic profile of detected Coliforms. The susceptibility of the isolates was tested using the Kirby-Bauer disc diffusion method on Mueller Hinton agar. A total of forty (40) isolates were isolated from the water samples of which (5) species were Gram Positive bacteria and 35 species of Gram Negative bacteria. The temperature for all ponds ranged from 25°C to 28°C. The mean bacteria count for pond C1 to T2 were 4.9 × 102, 4.9 × 102, 5.4 × 102, 2.5 × 102, 2.2 × 102, and 1.9 × 102 CFU/ml respectively. All isolates were 100% resistant to ceftazidime, cefuroxime and augmentin. More resistance to cefixime (80%) and gentamicin (73.3%) and nitrofurantoin (66.7%) was recorded. However, only 16.6% and 8.3% of the isolates were resistant to ciprofloxacin and ofloxacin respectively. The multiple antimicrobial resistance index (MARI) ranged from 0.5 to 0.9. The water quality parameters (temperature and pH) and the type of bacteria detected in all pond type did not differ significantly. The Multi-drug resistance bacteria detected could be pathogenic to fish and consumers.

Effects of pond structural complexity on the reproduction of Physalaemus ephippifer (Anura, Leptodactylidae)

Habitat structure is a factor that strongly affects the distribution of organisms. Habitats with high structural complexity provide a high number of microhabitats, allowing the coexistence of species with different behavioral, ecological and physiological requirements. We analyzed the effects of structural complexity of ponds on the number of males and foam nests of Physalaemus ephippifer, and the substrates to which individuals attach their foam nests. We sampled 41 ponds and recorded the number of individuals of P. ephippifer and environmental data (type of vegetation in, adjacent and around the pond; type of bottom substrate in the pond) to measure the degree of structural complexity of the ponds through an index ranging between 0 and 1. We found a positive influence of pond structural complexity on mean number of calling males, but not on number of foam nests. We found most foam nests attached to gramineans and herbaceous plants. Vegetation is an important component of habitats with high complexity, providing refuges for adults and tadpoles. The index was an efficient tool to measure habitat structural complexity, and may be used in further studies with other species.

Phytoplankton response to fish-induced environmental changes in a temperate shallow pond-type lake

Since 1967, the temperate, shallow, pond-type Lake Warniak has been subjected to different biomanipulation methods including the introduction of common carp,Cyprinus carpioL., grass carp,Ctenopharyngodon idella(Val.), silver carp,Hypophthalmichthys molitrix(Val.), and bighead carp,Hypophthalmichthys nobilis(Richardson) and then their removal in an effort to control macrophytes and phytoplankton. Recently, pilot stocking with predatory fish, particularly pike,Esox luciusL., has also been conducted. Hence, an examination of the long-term response patterns of phytoplankton to multiple fish-induced stressors was undertaken. In recent years,Charadomination (2000-2004) has helped to stabilize a clear-water state, high/good ecological status, and meso-eutrophic conditions. After the disappearance of Charales in 2004, the rapid, unstable changes in phytoplankton biomass, structure, and biodiversity suggested a shift toward a turbid-water state. As a result, the phytoplankton assemblages changed from those dominated by cryptophytes Y+X2+X1+LO(2000-2004) through those dominated by cyanobacteria K (2005-2008), dinoflagellates LO+Y (2009-2011), and cryptophytes Y+LO+F+X2 (2012), to those dominated by diatoms D+K+P+A (2013-2014) with representative taxa that occur in nutrient-rich and/or nutrient-poor water bodies. The 1967-2014 changes indicated that four periods, two with clear-water state and two with turbid-water state, alternately, one after the other, resulted from different fish pressure. Higher autochthonous fish biomass was usually accompanied by lower phytoplankton biomass. In contrast, the introduction of Cyprinidae fish had a stimulating effect on summer phytoplankton dominated by cyanobateria. Among the nutrients, only phosphorus played an important role.