Fluorophore-functionalized graphene oxide with application in cell imaging

A fluorescent carbon material with excellent fluorescence performances and a nearly nucleus-staining was prepared by a simple method.

Bright red-emitting pyrene derivatives with a large Stokes shift for nucleus staining

A red-emitting probe with a large Stokes shift (Δλ≈ 130 nm) exhibits great selectivity and sensitivity for cell nucleus imaging.

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

The fluorescence magnetic nanoparticles offer versatile platforms for nucleus imaging and DNA adsorption.

Nucleus-staining with biomolecule-mimicking nitrogen-doped carbon dots prepared by a fast neutralization heat strategy

Biomolecule-mimicking nitrogen-doped carbon dots were synthesized from dopamine for nucleus-staining by a neutralization heat strategy.

The development of a nucleus staining fluorescent probe for dynamic mitosis imaging in live cells

The rapid and efficient synthesis of a novel fluorescent xanthone library (AX) and its application for the development of a new nucleus staining fluorescent probe (CDb12) for monitoring real-time mitosis progression in live cells is presented.

A multifunctional phosphorescent iridium(iii) complex for specific nucleus staining and hypoxia monitoring

A multifunctional phosphorescent iridium(iii) complex has been synthesized for specific nucleus staining and hypoxia monitoring through time-resolved luminescence imaging.

The uterine environment modulates trophectodermal POU5F1 levels in equine blastocysts

The reported patterns of trophectodermal expression of POU5F1 protein in blastocysts vary among species, and are possibly related to the differences in placental growth and function. This study investigated the pattern of embryonic POU5F1 expression in the horse, a species with delayed placental formation. Immature equine oocytes expressed POU5F1 protein in the cytoplasm and nucleus. Staining for POU5F1 protein inin vitro-produced (IVP) embryos decreased to day 5 of culture, then the nuclear staining increased to day 7. IVP day-7 to -11 blastocysts showed POU5F1 staining in nuclei throughout the blastocysts. In contrast,in vivo-produced day-7 to -10 blastocysts showed greatly reduced trophoectodermal POU5F1 protein expression. To determine whether the uterine environment modulates POU5F1 expression, IVP blastocysts were transferred to the uteri of mares, then recovered 2–3 days later (IVP-ET embryos). These embryos showed similar POU5F1 expression as thein vivo-produced embryos. Levels ofPOU5F1,SOX2, andNANOGmRNA in IVP-ET blastocysts were significantly higher in the inner cell mass than in trophectoderm (TE) cells. These data suggest that the differentiation of equine TE, as indicated by loss of POU5F1 expression, is impaired duringin vitroculture, but proceeds normally when the embryos are exposed to the uterine environment. Previously reported differences in trophectodermal expression of POU5F1 among species may thus be in part artifactual, i.e. related toin vitroculture. Failure for correction of such changes by the uterine environment is a potential factor in the placental abnormalities seen after transfer of cultured embryos in some species.