scholarly journals Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

RSC Advances ◽  
2017 ◽  
Vol 7 (10) ◽  
pp. 5937-5947 ◽  
Author(s):  
Chi-Hsien Liu ◽  
Min-Han Tsao ◽  
Soubhagya Laxmi Sahoo ◽  
Wei-Chi Wu
Keyword(s):  
Magnetic Nanoparticles ◽  
Dna Sensing ◽  
Dna Adsorption ◽  
Nucleus Staining

The fluorescence magnetic nanoparticles offer versatile platforms for nucleus imaging and DNA adsorption.

Adsorption Behaviors of Nucleic Acid by Cationic Magnetic Nanoparticles

2013 ◽  
Vol 284-287 ◽  
pp. 271-275
Author(s):  
Chi Hsien Liu ◽  
Yi Fan Hsien
Keyword(s):  
Electron Microscopy ◽  
Magnetic Nanoparticles ◽  
Plasmid Dna ◽  
Langmuir Model ◽  
Dna Adsorption ◽  
Adsorption Behaviors ◽  
Adsorption Capacities ◽  
Transmission Electron ◽  
Modified Magnetic Nanoparticles ◽  
Surface Modified

Cationic magnetic nanoparticles are prepared by covalently binding spermidine and polyethylenimine onto the surface of nanoparticles via a glutaraldehyde coupling method. Nanoparticles modified by spermidine or polyethylenimine were characterized using Fourier-transformed infrared spectra, transmission electron microscopy and scanning electron microscopy. In this study, cationic magnetic nanoparticles were prepared by covalently conjugating cationic ligands onto the surface of nanoparticles. The plasmid DNA adsorption by the surface-modified magnetic nanoparticles was analyzed by Freundlich, Temkin, and Langmuir models. The maximal adsorption capacities in Langmuir model for polyethylenimine- and spermidine- modified nanoparticles are 341 and 116 μg/mg, respectively. Overall, the results demonstrated that the polyethylenimine-modified magnetic nanoparticles has the potential for purification of plasmid.

Contributions of Phosphate to DNA Adsorption/Desorption Behaviors on Aminosilane-Modified Magnetic Nanoparticles

Langmuir ◽  
2009 ◽  
Vol 25 (5) ◽  
pp. 2956-2961 ◽  
Author(s):  
Tsuyoshi Tanaka ◽  
Ririko Sakai ◽  
Ryosuke Kobayashi ◽  
Keiichi Hatakeyama ◽  
Tadashi Matsunaga
Keyword(s):  
Magnetic Nanoparticles ◽  
Dna Adsorption ◽  
Modified Magnetic Nanoparticles ◽  
Adsorption Desorption

Thermal Conductivity of Nanofluid with Magnetic Nanoparticles

PIERS Online ◽  
2009 ◽  
Vol 5 (3) ◽  
pp. 231-234 ◽  
Author(s):  
Tsung-Han Tsai ◽  
Long-Sheng Kuo ◽  
Ping-Hei Chen ◽  
Chin-Ting Yang
Keyword(s):  
Thermal Conductivity ◽  
Magnetic Nanoparticles

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

Magnetic Nanoparticles for Extracting DNA from Blood Cells

2020 ◽  
Vol 84 (11) ◽  
pp. 1362-1365
Author(s):  
A. V. Komina ◽  
R. N. Yaroslavtsev ◽  
Y. V. Gerasimova ◽  
S. V. Stolyar ◽  
I. A. Olkhovsky ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Blood Cells

MR Molecular Imaging Based on Magnetic Nanoparticles

2018 ◽  
pp. 17-28
Author(s):  
Hwunjae Lee ◽  
◽  
SangBock Lee ◽  
Geahwan Jin ◽  
Sergey NETESOV ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Molecular Imaging
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