13c tracer
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2021 ◽  
Vol 96 (12) ◽  
pp. 124023
Author(s):  
Tomi Vuoriheimo ◽  
Antti Hakola ◽  
Jari Likonen ◽  
Sebastijan Brezinsek ◽  
Timo Dittmar ◽  
...  
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2021 ◽  
Author(s):  
Jin Wook Cha ◽  
Xing Jin ◽  
Sihyang Jo ◽  
Yong Jin An ◽  
Sunghyouk Park

Metabolomic isotopic tracing can provide flux information useful for understanding drug mechanisms. For that, NMR has the unique advantage of giving positional isotope enrichment information, but the current 13C 1D...


2020 ◽  
Vol 25 ◽  
pp. 100805
Author(s):  
S. Möller ◽  
R. Ding ◽  
H. Xie ◽  
B.F. Gao ◽  
B.G. Wang ◽  
...  

2019 ◽  
Vol 1080 ◽  
pp. 127-137 ◽  
Author(s):  
Xueni Sun ◽  
Paul Heinrich ◽  
Raffaela S. Berger ◽  
Peter J. Oefner ◽  
Katja Dettmer

Metabolites ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 63 ◽  
Author(s):  
André Feith ◽  
Attila Teleki ◽  
Michaela Graf ◽  
Lorenzo Favilli ◽  
Ralf Takors

Dynamic 13C-tracer-based flux analyses of in vivo reaction networks still require a continuous development of advanced quantification methods applying state-of-the-art mass spectrometry platforms. Utilizing alkaline HILIC chromatography, we adapt strategies for a systematic quantification study in non- and 13C-labeled multicomponent endogenous Corynebacterium glutamicum extracts by LC-QTOF high resolution (HRMS) and LC-QQQ tandem mass spectrometry (MS/MS). Without prior derivatization, a representative cross-section of 17 central carbon and anabolic key intermediates were analyzed with high selectivity and sensitivity under optimized ESI-MS settings. In column detection limits for the absolute quantification range were between 6.8–304.7 (QQQ) and 28.7–881.5 fmol (QTOF) with comparable linearities (3–5 orders of magnitude) and enhanced precision using QQQ-MRM detection. Tailor-made preparations of uniformly (U)13C-labeled cultivation extracts for isotope dilution mass spectrometry enabled the accurate quantification in complex sample matrices and extended linearities without effect on method parameters. Furthermore, evaluation of metabolite-specific m+1-to-m+0 ratios (ISR1:0) in non-labeled extracts exhibited sufficient methodical spectral accuracies with mean deviations of 3.89 ± 3.54% (QTOF) and 4.01 ± 3.01% (QQQ). Based on the excellent HILIC performance, conformity analysis of time-resolved isotopic enrichments in 13C-tracer experiments revealed sufficient spectral accuracy for QQQ-SIM detection. However, only QTOF-HRMS ensures determination of the full isotopologue space in complex matrices without mass interferences.


Author(s):  
Marit van Gorsel ◽  
Ilaria Elia ◽  
Sarah-Maria Fendt
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2016 ◽  
Vol 100 ◽  
pp. 182-191 ◽  
Author(s):  
Ivana Lozanovska ◽  
Yakov Kuzyakov ◽  
Johannes Krohn ◽  
Shahnaj Parvin ◽  
Maxim Dorodnikov

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