cag pai
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Author(s):  
Samuel D R Dooyema ◽  
Uma S Krishna ◽  
John T Loh ◽  
Giovanni Suarez ◽  
Timothy L Cover ◽  
...  

Abstract Helicobacter pylori is the strongest risk factor for gastric adenocarcinoma. The H. pylori cancer-associated cag pathogenicity island (cag-PAI) encodes a type IV secretion system (T4SS) which translocates microbial DNA and activates TLR9; however, most cag-PAI + infected persons do not develop cancer and cag-PAI-independent regulators of pathogenesis, including strain-specific adhesins, remain understudied. We defined the relationships between H. pylori HopQ adhesin allelic type, gastric injury, and TLR9 activation. Type I hopQ alleles were significantly associated with magnitude of injury, cag-T4SS function, and TLR9 activation. Genetic deletion of hopQ significantly decreased H. pylori-induced TLR9 activation, implicating this adhesin in H. pylori-mediated disease.


2019 ◽  
Vol 8 (10) ◽  
pp. 4928-4937 ◽  
Author(s):  
Seyedeh Zahra Bakhti ◽  
Saeid Latifi‐Navid ◽  
Saber Zahri ◽  
Abbas Yazdanbod

2018 ◽  
Vol 59 ◽  
pp. 167-171 ◽  
Author(s):  
Rumyana Markovska ◽  
Lyudmila Boyanova ◽  
Daniel Yordanov ◽  
Petya Stankova ◽  
Galina Gergova ◽  
...  

2018 ◽  
Vol 08 (03) ◽  
Author(s):  
Firouzeh Morshedzadeh ◽  
Hossein Abbasinia ◽  
Davood Zaeifi

2017 ◽  
Vol 145 (16) ◽  
pp. 3468-3476 ◽  
Author(s):  
J. YAKOOB ◽  
Z. ABBAS ◽  
Z. AHMAD ◽  
K. TARIQ ◽  
S. AWAN ◽  
...  

SUMMARYB-cell non-Hodgkin lymphoma (B-cell NHL) is the second commonest malignancy in the stomach. We determined the distribution ofHelicobacter pyloriouter membrane protein Q (HopQ) allelic type, cytotoxin-associated gene (cag)-pathogenicity activity island (cag-PAI) and vacuolation activating cytotoxin A (vacA) genes, respectively, in patients with B-cell NHL. We also compared them with their distribution in non-ulcer dyspepsia (NUD).H. pyloriwas cultured from gastric biopsy tissue obtained at endoscopy. Polymerase chain reaction was performed. Of 170 patients enrolled, 114 (63%) had NUD and 56 (37%) had B-cell NHL. HopQ type 1 was positive in 66 (58%) in NUD compared with 46 (82%) (P= 0·002) in B-cell NHL; HopQ type 2 was positive in 93 (82%) with NUD compared with 56 (100%) (P< 0·001) in B-cell NHL. Multiple HopQ types were present in 46 (40%) in NUD compared with 46 (82%) (P< 0·001) in B-cell NHL. CagA was positive in 48 (42%) in NUDvs. 50 (89%) (P< 0·001) in B-cell NHL; cagT was positive in 35 (31%) in NUDvs. 45 (80%) (P< 0·001) in B-cell NHL; left end of thecagAgene (LEC)1 was positive in 23 (20%) in NUDvs. 43 (77%) (P< 0·001) in B-cell NHL. VacAs1am1 positive in B-cell NHL in 48 (86%) (P< 0·001)vs. 50 (44%) in NUD, while s1am2 was positive in 20 (17%) in NUDvs. 46 (82%) (P< 0·001) in B-cell NHL.H. pyloristrains with multiple HopQ allelic types, truncated cag-PAI evidenced by expression of cagA, cagT and cag LEC with virulent vacAs1 alleles are associated with B-cell NHL development.


2017 ◽  
Vol 48 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Xiao-yan Yuan ◽  
Jin-Jun Yan ◽  
Ya-chao Yang ◽  
Chun-mei Wu ◽  
Yan Hu ◽  
...  

2015 ◽  
Vol 63 (6) ◽  
pp. 252-257 ◽  
Author(s):  
A. Ahmadzadeh ◽  
H. Ghalehnoei ◽  
N. Farzi ◽  
A. Yadegar ◽  
M. Alebouyeh ◽  
...  

2015 ◽  
Vol 309 (3) ◽  
pp. G193-G201 ◽  
Author(s):  
Charles E. Hammond ◽  
Craig Beeson ◽  
Giovanni Suarez ◽  
Richard M. Peek ◽  
Steffen Backert ◽  
...  

Acute Helicobacter pylori infection of gastric epithelial cells and human gastric biopsies represses H,K-ATPase α subunit (HKα) gene expression and inhibits acid secretion, causing transient hypochlorhydria and supporting gastric H. pylori colonization. Infection by H. pylori strains deficient in the cag pathogenicity island ( cag PAI) genes cagL, cagE, or cagM, which do not transfer CagA into host cells or induce interleukin-8 secretion, does not inhibit HKα expression, nor does a cagA-deficient strain that induces IL-8. To test the hypothesis that virulence factors other than those mediating CagA translocation or IL-8 induction participate in HKα repression by activating NF-κB, AGS cells transfected with HKα promoter- Luc reporter constructs containing an intact or mutated NF-κB binding site were infected with wild-type H. pylori strain 7.13, isogenic mutants lacking cag PAI genes responsible for CagA translocation and/or IL-8 induction ( cagA, cagζ, cagε, cagZ, and cagβ), or deficient in genes encoding two peptidoglycan hydrolases ( slt and cagγ). H. pylori-induced AGS cell HKα promoter activities, translocated CagA, and IL-8 secretion were measured by luminometry, immunoblotting, and ELISA, respectively. Human gastric biopsy acid secretion was measured by microphysiometry. Taken together, the data showed that HKα repression is independent of IL-8 expression, and that CagA translocation together with H. pylori transglycosylases encoded by slt and cagγ participate in NF-κB-dependent HKα repression and acid inhibition. The findings are significant because H. pylori factors other than CagA and IL-8 secretion are now implicated in transient hypochlorhydria which facilitates gastric colonization and potential triggering of epithelial progression to neoplasia.


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