Evaluation of the Antimicrobial and Antioxidant Activity of Two Chlorophyceae and Two Rhodophyceae Seaweeds from Porbandar Coast

Seaweeds are significant marine sources of bioactive compounds with prospective use inefficient foods and nutraceutical products. Four marine macroalgae species from the western coasts of Gujarat were evaluated for their antimicrobial and antioxidant activity. The antimicrobial activity of extracts of red seaweed Hypnea valentiae and Spyridia filamentosa and green seaweed Enteromorpha compressa and Caulerpa racemosa using cold percolation extraction in an in-vitro method and testing against six pathogenic bacteria and one fungi. Results showed effective inhibition zone. The antioxidant activity of the seaweeds methanol extracts was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay for measuring potential scavenger of free radical. Both activities were shown promising result of all four seaweeds. This study suggested that seaweeds could have shown a potential role in the future remedy and nutrition which can be used as drug or food additive.

Antioxidant Properties of R-Phycoerythrin from Red Alga Spyridia filamentosa (Wulfen) Harvey Collected on the Pudumadam Coast

Natural antioxidants are found in algae, are important biologically active compounds that involve in protecting the cells from oxidation thereby used in defense mechanism on ageing processes and various diseases. In this study, R-Phycoerythrin (R-PE) was extracted from Spyridia filamentosa and was assessed for their antioxidant properties. The antioxidising property of decontaminated R-PE has been evaluated by measuring the free radical scavenging activity by DPPH radical scavenging assay, ABTS radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical (O2) scavenging assay, nitric oxide scavenging activity and phosphomolybdenum reduction assay. The purified R-PE extract was subjected to structural analysis by UV-Visible spectroscopy, fluorescence emission spectroscopy and infrared spectroscopy. The UV-Visible spectrum showed a peak at 496 nm, 543 nm and 564 nm and fluorescence emission spectrum of R-PE was measured 575 nm which confirmed the presence of R-Phycoerythrin. The IR spectral data showed the presence of aromatic constituents containing amide group in the protein. DPPH radical scavenging assay showed the highest antioxidant potential with an IC50 value of 128.25 μg/ml. The radical scavenging activity of Nitric oxide showed an IC50 value of 87.85 μg/ml, Phosphomolybdenum reduction assay showed an RC50 value of 61.45 μg/ml. The hydroxyl radical scavenging activity showed IC50 value of 34.56 μg/ml and Superoxide radical (O2) scavenging assay showed IC50 value of 18.58 μg/ml. The ABTS radical scavenging activity showed the lowest antioxidant potential of 3.13 μg/ml. The free radical scavenging property of R-Phycoerythrin (R-PE) concentrate of Spyridia filamentosa demonstrate that it has high antioxidant potency and it could be considered as utilitarian nourishment with essential pharmaceutical and biological importance.

JENIS-JENIS MAKROALGA EPIFIT PADA BUDIDAYA (Kappaphycus alvarezii) DI PERAIRAN TELUK GERUPUK LOMBOK TENGAH

Epiphytes are organisms that attach to other organisms to support their growth and development. One type of epiphytes that has a negative impact on the host is algae (macroalgae). This algae is a competing organism for cultivation algae (Kappaphycus alvarezii). This research was conducted From May to October 2017 in Gerupuk Bay, Central Lombok. The aim of the research is to find out the types of macroalgae epiphytes on macroalgae kappaphycus alvarezii. This research was conducted using exploratory methods by taking seaweed samples overgrown with epiphytes, the research data obtained were descriptive analisys. As supporting data, environmental parameters are measured including temperature, pH, salinity, DO, phosphate, and NH4. Based on the results of exploration found 13 species of epiphytic algae in kappaphycus alvarezi cultivation, namely: Chaetomorpha linum, Chaetomorpha linum, Cladophora dalmatica, Acanthophora spicifer, Batrachospermum sp, Callithamnion sp., Caulachantus ustulatus, Ceramium sp, Hypnea spinnosa, Hypnea spinella, Hypnea sp, Polysiphonia brodiaei , Spyridia filamentosa. The type of epiphytes found that grows in both cultivation methods and some others are found only in one method. Meanwhile, data on environmental parameters are in the optimal range for macroalgae growth.This data is expected to be used as a consideration for the cultivateddevelopment in the Gerupuk Bay cultivation center. Keywords : Macroalgae, Epiphytes, Aquaculture, Kappaphycus alvarezii