Epigallocatechin gallate prevents senescence by alleviating oxidative stress and inflammation in WI-38 human embryonic fibroblasts

Increased levels of oxidative stress and inflammation are the underlying mechanisms behind the aging process and age-related diseases.

The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-induced Premature Senescence

Ovarian liquid and fish roe are valuable sources of biologically active compounds. In order to study mechanisms of aging and also to search for biologically active compounds capable of inhibiting aging, we have modelled premature senescence in human embryonic fibroblasts by incubating of cells with Н2О2. Cell senescence was assessed by staining for β-galactosidase (SA-β-Gal) at pH 6.0; cell proliferation was further evaluated by the optical method. The dried ovarian liquid of the Siberian sturgeon and the extract of the perch roe were able to enhance recovery of the cells after induction of their premature senescence caused by oxidative stress. In contrast to the extract from perch roe and the extracts from fish muscle, dried ovarian fluid gave such an effect without the growth of proliferation.