scholarly journals Reprogramming efficiency of human embryonic fibroblasts using polycistronic lentiviral expression of reprogramming factors OSKM. Testing of factors that enhance efficiency

2013 ◽  
Author(s):  
W.W.M. Pim Pijnappel ◽  
W.W.M. Pim Pijnappel ◽  
Erik van der Wal ◽  
Holm Zaehres ◽  
Hans. R. Schöler ◽  
...  
Keyword(s):  
Embryonic Fibroblasts ◽  
Reprogramming Efficiency ◽  
Reprogramming Factors ◽  
Enhance Efficiency ◽  
Human Embryonic Fibroblasts

scholarly journals Abortive Infection and Transformation of Human Embryonic Fibroblasts by Molluscum contagiosum Virus

1974 ◽  
Vol 24 (2) ◽  
pp. 237-246 ◽  
Author(s):  
G. Barbanti-Brodano ◽  
A. Mannini-Palenzona ◽  
O. Varoli ◽  
M. Portolani ◽  
M. La Placa
Keyword(s):  
Molluscum Contagiosum ◽  
Abortive Infection ◽  
Molluscum Contagiosum Virus ◽  
Embryonic Fibroblasts ◽  
Human Embryonic Fibroblasts

Colchicine-like action of sevine on human embryonic fibroblasts in vitro

1972 ◽  
Vol 73 (6) ◽  
pp. 703-705
Author(s):  
A. F. Vasilos ◽  
V. D. Dmitrienko ◽  
I. G. Shroit
Keyword(s):  
Embryonic Fibroblasts ◽  
Human Embryonic Fibroblasts

Abstract 33: Unlocking Reprogramming Capability: Silencing Antiplasticity Gene p63 Enhances the Reprogramming of Fibroblasts into Induced Cardiomyocytes

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Vivekkumar Patel ◽  
Austin Cooney ◽  
Elsa Flores ◽  
Vivek Singh ◽  
Megumi Mathison ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Troponin T ◽  
Cardiac Fibroblasts ◽  
Fold Increase ◽  
Cellular Reprogramming ◽  
Embryonic Fibroblasts ◽  
Reprogramming Efficiency ◽  
Cardiac Transcription Factors

Objective: In situ cellular reprogramming of cardiac fibroblasts into (induced) cardiomyocytes (iCMs) represents a promising new potential intervention for the treatment of heart failure. Despite encouraging in vivo data in rodent myocardial infarction models, the relative resistance of human cells to reprogramming may be a significant barrier to the clinical application of this new therapy. We hypothesized that knockdown of the anti-plasticity gene p63 could therefore be used to enhance cellular reprogramming efficiency. Methods: p63 knockout (KO) murine embryonic fibroblasts (MEFs) and MEFs treated with p63 silencing shRNA were assessed for expression of the cardiomyocyte marker Cardiac Troponin T (cTnT) and pro-cardiogenic genes, with or without the treatment with known cardiac transcription factors Hand2 and Myocardin (HM). Results: After 3 wks in culture, expression of the cardiomyocyte marker cTnT (FACS) was significantly greater in p63 KO MEFs than in wild-type (WT) MEFs or WT MEFs treated with transcription factors Hand2 and Myocardin (39% ± 8%, 2.0% ± 1% and 2.7 ± 0.3%, respectively, p < 0.05). Treatment of p63 KO MEFs with Hand2 and Myocardin further increased cTnT expression up to 74% ± 3%. Treatment of WT MEFs with p63 shRNA likewise yielded a 20-fold increase in cTnT expression (qPCR) without HM and a 600-fold increase with HM when compared to non-silencing shRNA treated MEFs. Consistent with these findings, p63 KO or p63 shRNA-treated MEFs demonstrated increased expression (qPCR) of pro-cardiogenic genes Gata4, Mef2c and Tbx5 compared to naïve or non-silencing shRNA treated MEFs. After treatment with p63 shRNA, adult human epidermal cells also demonstrated increased expression of cTnT, myosin heavy chain and pro-cardiogenic genes when analyzed by qPCR. Conclusions: Downregulation of the anti-plasticity gene p63 enhances cellular reprogramming efficiency and iCM generation, as reflected in the increased expression of the cardiomyocyte marker cTnT and pro-cardiogenic genes Gata4, Mef2c and Tbx5. Use of such cellular plasticity enhancing strategies may be a useful strategy to overcome barriers to cellular reprogramming in the clinical arena.

scholarly journals Epigallocatechin gallate prevents senescence by alleviating oxidative stress and inflammation in WI-38 human embryonic fibroblasts

RSC Advances ◽  
2019 ◽  
Vol 9 (46) ◽  
pp. 26787-26798 ◽  
Author(s):  
Qiao Zhang ◽  
Yuqing Wu ◽  
Yue Guan ◽  
Fan Ling ◽  
Ying Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aging Process ◽  
Epigallocatechin Gallate ◽  
Embryonic Fibroblasts ◽  
Age Related ◽  
Underlying Mechanisms ◽  
Human Embryonic Fibroblasts

Increased levels of oxidative stress and inflammation are the underlying mechanisms behind the aging process and age-related diseases.

scholarly journals Restoration of down-regulated PDGF receptors by TGF-β in human embryonic fibroblasts

FEBS Letters ◽  
1994 ◽  
Vol 339 (1-2) ◽  
pp. 84-88 ◽  
Author(s):  
Stelios Psarras ◽  
Dimitris Kletsas ◽  
Dimitri Stathakos
Keyword(s):  
Embryonic Fibroblasts ◽  
Human Embryonic Fibroblasts

Impaired oxidation of pyruvate in human embryonic fibroblasts after exposure to L-dopa

1994 ◽  
Vol 263 (1-2) ◽  
pp. 157-162 ◽  
Author(s):  
Peter Werner ◽  
Catherine Mytilineou ◽  
Gerald Cohen ◽  
Melvin D. Yahr
Keyword(s):  
Embryonic Fibroblasts ◽  
Human Embryonic Fibroblasts
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