Magnetic cellulose beads as support for enzyme immobilization using polyelectrolytes through electrostatic adsorption

Pectinase was immobilized on magnetic regenerated cellulose microspheres using cationic polyacrylamide (CPAM) or polyethyleneimine (PEI) via electrostatic adsorption. PEI-immobilized enzyme (PEI-EMCB) had higher activity (2711 U/g) than CPAM-immobilized enzyme (CPAM-EMCB). The time course of PEI-immobilized enzyme catalytic reaction was similar to the free form. PEI-EMCB reached its maximal activity at pH 4.8, while the optimal pH for CPAM-EMCB was the same with the free form (4.4). CPAM3-EMCB was most tolerant to pH variation, which can be possibly ascribed to the high molecular weight of CPAM3. After immobilization, the optimum temperature for the enzyme declined from 45 °C to different degrees. PEI-EMCB exhibited good storage stability with 67% of the initial activity maintained after 7 days and with moderate reusability. The magnetic properties of the regenerated cellulose beads provided convenience for the immobilized enzyme to be used and recycled. The results indicate a potential route for utilization of cellulose as enzyme support via a simple method.

Application of Management Bionics to Technological Innovation in Agriculture

A study was done on agricultural enterprises technology innovation based on the management bionics method. From the perspective of bionics, this study investigates influence factors, mechanisms and team building of agricultural enterprises technology innovation based on the scientific and normative analysis of management bionics. Through standardized management bionic analogy analysis, the following analogies were drawn: agricultural enterprises technology innovation influence factors and the onion biological form, agricultural enterprises technology innovation mechanism and the biological enzyme catalytic reaction mechanism, agricultural enterprises technology innovation team building with the bee biological group effect. Therefore, three bionic element models of agricultural enterprises technology innovation were built, namely agricultural enterprises technology innovation onion model, technology innovation enzyme catalytic reaction bionic model and the bees’ team effect bionic model. This study ends with the corresponding bionic strategies and suggestions about agricultural enterprises technology innovation, which may provide references for the development of agricultural enterprises technology innovation in China. At the same time, this study also enriches the research range and theoretical system of management bionics.

Detection of L-Tert-Leucine in the Enzyme-Catalyzed Reaction System

Direct Determination of L-Ter-Leucine in Enzyme Catalytic Reaction System by HPLC Was Studied. the Detection Were Performed on a Kromasil 700-5C18 Column Using a Eluant Containing 0.25% (NH4)H2PO4 and 100% Methanol (V((NH4)H2PO4):V(methanol)=100:5) with the Flow Rate of 0.8 Ml/min at,detection Wavelength of 205nm. there Was a Good Line Correlation between Peak Area and Contents in the Rang of 0.2-10 Mg/ml, the Correlation Coefficient Was 0.9986, the Average Recovery Was 98.88% with a Relative Stand Deviation of 0.78% (n=5). this Method Is Simple, Stable, Accurate and Reliable for the Quality Control of L–ter-Leucine.