An Isothermal Titration Calorimetric Method to Determine the Kinetic Parameters of Enzyme Catalytic Reaction by Employing the Product Inhibition as Probe

2001 ◽  
Vol 299 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Lifeng Cai ◽  
Aoneng Cao ◽  
Luhua Lai
Keyword(s):  
Kinetic Parameters ◽  
Catalytic Reaction ◽  
Product Inhibition ◽  
Calorimetric Method ◽  
Enzyme Catalytic Reaction

Detection of L-Tert-Leucine in the Enzyme-Catalyzed Reaction System

2013 ◽  
Vol 641-642 ◽  
pp. 717-720
Author(s):  
Sheng Deng ◽  
Li Cui ◽  
Li Min Ma
Keyword(s):  
Quality Control ◽  
Correlation Coefficient ◽  
Flow Rate ◽  
Catalytic Reaction ◽  
Reaction System ◽  
Direct Determination ◽  
Average Recovery ◽  
Enzyme Catalytic Reaction

Direct Determination of L-Ter-Leucine in Enzyme Catalytic Reaction System by HPLC Was Studied. the Detection Were Performed on a Kromasil 700-5C18 Column Using a Eluant Containing 0.25% (NH4)H2PO4 and 100% Methanol (V((NH4)H2PO4):V(methanol)=100:5) with the Flow Rate of 0.8 Ml/min at,detection Wavelength of 205nm. there Was a Good Line Correlation between Peak Area and Contents in the Rang of 0.2-10 Mg/ml, the Correlation Coefficient Was 0.9986, the Average Recovery Was 98.88% with a Relative Stand Deviation of 0.78% (n=5). this Method Is Simple, Stable, Accurate and Reliable for the Quality Control of L–ter-Leucine.

scholarly journals Consequences of product inhibition in the quantification of kinetic parameters

2020 ◽  
Vol 389 ◽  
pp. 468-475 ◽  
Author(s):  
James W. Harris ◽  
Anuj A. Verma ◽  
Jeremy W. Arvay ◽  
Arthur J. Shih ◽  
W. Nicholas Delgass ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Product Inhibition

scholarly journals Glucose-6-phosphate dehydrogenase variants: reexamination of G6PD Chicago and Cornell and a new variant (G6PD Pea Ridge) resembling G6PD Chicago

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 216-220
Author(s):  
VF Fairbanks ◽  
AG Nepo ◽  
E Beutler ◽  
ER Dickson ◽  
G Honig
Keyword(s):  
Kinetic Parameters ◽  
Electrophoretic Mobility ◽  
Hemolytic Anemia ◽  
Product Inhibition ◽  
Heterogeneous Group ◽  
New Variant ◽  
Glucose 6 Phosphate Dehydrogenase

Two large and unrelated families were investigated for hereditary nonspherocytic hemolytic anemia associated with deficiency of erythrocyte glucose-6-phosphate dehydrogenase (G6PD). In both families, the kinetic and electrophoretic features of the G6PD variants resembled those of G6PD Chicago. Further investigation revealed that members of one of these families previously had been characterized as having the G6PD variants Chicago and Cornell. However, it is clear that each of these terms has been applied to the same variant in this single large kindred. In the second family, we describe a newly identified variant with unique characteristics, which we have designated G6PD Pea Ridge. G6PD Pea Ridge resembles G6PD Chicago but differs in electrophoretic mobility and in a few kinetic parameters. It exhibits an unusually high Ki for NADPH and thus appears to be insensitive to product inhibition. As other cases previously considered to be the Chicago variant become more fully characterized, this probably will be shown to be a heterogeneous group of variants.

Conversion of Synthesis Gas into Light Hydrocarbons. Modelling of the Catalytic Reaction Network

2004 ◽  
Vol 2 (1) ◽  
Author(s):  
Louis Hagey ◽  
Hugo Ignacio de Lasa
Keyword(s):  
Kinetic Model ◽  
Kinetic Parameters ◽  
Gas Phase ◽  
Statistical Methods ◽  
Synthesis Gas ◽  
Catalytic Reaction ◽  
Reaction Network ◽  
Light Hydrocarbons ◽  
Model Based

This study considers a bi-functional catalyst for the conversion of synthesis gas into valuable light hydrocarbons. Catalysts are tested in a well-mixed Berty reactor. The effects of phosphorous additions on the overall catalyst performance including C5+ fractions and hydrocarbon yields are evaluated. The data obtained is very useful for establishing the validity of a kinetic model based on the gas phase observable species and to determine the various kinetic parameters of this model using statistical methods.

Application of Management Bionics to Technological Innovation in Agriculture

2013 ◽  
Vol 461 ◽  
pp. 482-490
Author(s):  
Yin Sheng Yang ◽  
Yue Meng Jiang ◽  
Hai Na Wang
Keyword(s):  
Catalytic Reaction ◽  
Technology Innovation ◽  
Team Building ◽  
Influence Factors ◽  
Agricultural Enterprises ◽  
Biological Form ◽  
Enzyme Catalytic Reaction ◽  
Innovation Mechanism ◽  
Biological Group ◽  
Catalytic Reaction Mechanism

A study was done on agricultural enterprises technology innovation based on the management bionics method. From the perspective of bionics, this study investigates influence factors, mechanisms and team building of agricultural enterprises technology innovation based on the scientific and normative analysis of management bionics. Through standardized management bionic analogy analysis, the following analogies were drawn: agricultural enterprises technology innovation influence factors and the onion biological form, agricultural enterprises technology innovation mechanism and the biological enzyme catalytic reaction mechanism, agricultural enterprises technology innovation team building with the bee biological group effect. Therefore, three bionic element models of agricultural enterprises technology innovation were built, namely agricultural enterprises technology innovation onion model, technology innovation enzyme catalytic reaction bionic model and the bees’ team effect bionic model. This study ends with the corresponding bionic strategies and suggestions about agricultural enterprises technology innovation, which may provide references for the development of agricultural enterprises technology innovation in China. At the same time, this study also enriches the research range and theoretical system of management bionics.

scholarly journals Glucose-6-phosphate dehydrogenase variants: reexamination of G6PD Chicago and Cornell and a new variant (G6PD Pea Ridge) resembling G6PD Chicago

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 216-220 ◽  
Author(s):  
VF Fairbanks ◽  
AG Nepo ◽  
E Beutler ◽  
ER Dickson ◽  
G Honig
Keyword(s):  
Kinetic Parameters ◽  
Electrophoretic Mobility ◽  
Hemolytic Anemia ◽  
Product Inhibition ◽  
Heterogeneous Group ◽  
New Variant ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract Two large and unrelated families were investigated for hereditary nonspherocytic hemolytic anemia associated with deficiency of erythrocyte glucose-6-phosphate dehydrogenase (G6PD). In both families, the kinetic and electrophoretic features of the G6PD variants resembled those of G6PD Chicago. Further investigation revealed that members of one of these families previously had been characterized as having the G6PD variants Chicago and Cornell. However, it is clear that each of these terms has been applied to the same variant in this single large kindred. In the second family, we describe a newly identified variant with unique characteristics, which we have designated G6PD Pea Ridge. G6PD Pea Ridge resembles G6PD Chicago but differs in electrophoretic mobility and in a few kinetic parameters. It exhibits an unusually high Ki for NADPH and thus appears to be insensitive to product inhibition. As other cases previously considered to be the Chicago variant become more fully characterized, this probably will be shown to be a heterogeneous group of variants.

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