Prevalensi dan Tingkat Kontaminasi Listeria monocytogenes di Tambak dan Unit Pengolahan Udang Vaname (Litopenaeus vannamei) untuk Pasar Ekspor

Listeria monocytogenes adalah salah satu bakteri patogen yang dapat menyebabkan penyakit bawaan pangan. Penolakan ekspor produk udang beku Indonesia karena kontaminasi L. monocytogenes masih terjadi yang berdampak pada kerugian material bagi pelaku usaha. Tujuan penelitian ini adalah untuk mengetahui prevalensi dan tingkat kontaminasi L. monocytogenes pada produk udang beku untuk pasar ekspor. Sampel yang diambil merupakan udang segar dari tambak dan bahan baku dari bagian penerimaan di Unit Pengolahan Ikan (UPI) serta udang beku sebagai produk akhir UPI, dengan menerapkan sistem ketertelusuran. Lokasi penelitian adalah Sumatra Utara (Medan), DKI Jakarta, Jawa Timur (Surabaya dan Banyuwangi), dan Sulawesi Selatan (Makassar). Identifikasi dan enumerasi L. monocytogenes dilakukan dengan metode MPN-PCR dengan target gen hlyA (~456bp). Prevalensi L. monocytogenes pada udang vaname secara keseluruhan sebesar 6,7% (9/135 sampel), dengan prevalensi di masing-masing titik pengambilan sampel berturut-turut 6,1% di tambak, 9,6% di bahan baku, dan 4% di produk akhir, yang merupakan sampel udang dari batch yang sama. Tingkat kontaminasi L. monocytogenes pada sampel udang vaname berkisar 6,1-1.100 APM/g. Persyaratan L. monocytogenes pada bahan pangan adalah negatif/25g, sehingga sampel udang yang terkontaminasi L. monocytogenes tersebut tidak memenuhi persyaratan sebagai pangan yang aman untuk dikonsumsi berdasarkan regulasi yang berlaku di Indonesia maupun di negara lain. Kontaminasi L. monocytogenes pada udang beku kemungkinan berasal dari tambak ataupun lingkungan pengolahan. Penerapan Good Aquaculture Practices (GAP) di lingkungan tambak udang, serta Hazard Analysis Critical Control Point (HACCP) dan Good Manufacturing Practices (GMP) di UPI perlu dilakukan dengan benar sebagai upaya pengendalian kontaminasi L. monocytogenes. Selain itu, perlu dilakukan kajian lebih lanjut mengenai sumber dan titik kritis kontaminasi L. monocytogenes di sepanjang rantai pengolahan udang beku mulai dari tambak sampai produk akhir.ABSTRACTListeria monocytogenes is pathogenic bacteria that can cause foodborne illness. Rejection of frozen shrimp exports due to L. monocytogenes contamination still occurs and causes economical losses for the industries. The objective of this study was to determine the prevalence and the level of L. monocytogenes contamination in frozen shrimp for export markets. Samples collected were fresh shrimp from shrimp culture and raw material from the receiving point of fish processing plants (UPI), and frozen shrimp as the end product, by implementing a traceability system. Study locations were in North Sumatra (Medan), Special Capital Region of Jakarta, East Java (Surabaya dan Banyuwangi), and South Sulawesi (Makassar). Identification and enumeration of L. monocytogenes were carried out using the MPN-PCR method with the target gene hlyA (~456bp). The prevalence of L. monocytogenes in vanname shrimp was 6.7% (9 out of 135 samples), where 6.1%, 9.6%, and 4% of the prevalence were found in samples from shrimp culture, raw material, and end product, respectively. These samples were from the same batch. The contamination level ranged from 6.1 to 1,100 MPN/g. L. monocytogenes in food should be negative/25g, thus the contaminated samples do not meet requirements as safe for human consumption based on food regulation in Indonesia and other countries. Findings from this study suggested that shrimp culture or fish processing environment are potential sources of L. monocytogenes contamination in frozen shrimp. Therefore, the implementation of Good Aquaculture Practices (GAP) in shrimp culture environment, as well as Hazard Analysis Critical Control Point (HACCP) and Good Manufacturing Practices (GMP) in shrimp processing plant are necessary to control L. monocytogenes contamination. Further studies regarding the sources and critical points of L. monocytogenes contamination throughout the processing of frozen shrimp from shrimp culture to end product are also needed.

Bioconversion of Shrimp Waste with Fermentation Stage Process on Proximate Analysis and Digestibility Values of Feed

Frozen shrimp processing waste has the potential to be used as feed, but the characteristics of the shrimp shells need to be improved so that they can be digested. Fermentation using three types of microbes in stages has been studied, to determine the optimal processing time that yields proximate values and protein digestibility of shrimp waste concentrate. Completely randomized design (CRD), 3 treatments and 7 replications, conducted with treatments of shrimp waste bioconversion in stage over time, T1 = Bacillus licheniformis (Bl.) 1 day + Lactobacillus sp. (Ls.) 1 day; + Saccharomyces cerevisiae (Sc) 1 day; T2 = Bl. 2 days + Ls. 2 days + Sc = 2 days; T3 = Bl. 3 days + Ls. 3 days + Sc = 3 days. Product of shrimp waste bioconversion was used as a nutrient concentrate in dietary of local poultry (CP 15%, ME 2750 kcal/kg). The best proximate analysis value showed that each stage overtime two days of bioconversion with Bacillus licheniformis, followed by Lactobacillus sp. and finally fermented by Saccharomyces cerevisiae. The proximate analysis resulted that the Crude Protein of product bioconversion was 48.5%. Extract ether, Calcium and Phosphorous contents respectively were 7.81%, 7.57% and 3.14%. The best value of digestibility of protein feed containing bioconversion product of concentrations of local poultry was 72.91%.

PENGOLAHAN UDANG PUTIH (Litopenaeus vannamei) KUPAS MENTAH BEKU DI PT. XXX, PONTIANAK-KALIMANTAN BARAT

ABSTRAK Udang vaname (Litopenaeus vannamei) merupakan udang yang saat ini dikembangkan di Indonesia. Produk udang beku merupakan produk yang digemari oleh masyarakat Indonesia. Tujuan dilakukannya penelitian ini yaitu untuk mengetahui mutu bahan baku udang, penerapan rantai dingin dalam proses pengolahan udang PND, mutu produk akhir, rendemen serta produktivitas tenaga kerja di PT.Pulau Mas Khatulistiwa. Bahan baku udang masih tergolong kedalam kategori udang segar dikarenakan rata-rata nilai organoleptiknya 7,6. Kandungan mikroba dalam bahan baku udang masih sesuai dengan standar SNI. Dalam bahan baku udang tidak ditemukan jenis antibiotik kloramfenikol, nitrofuran, maupun tetrasiklin. Produk akhir udang PND memiliki nilai organoleptik 8, kandungan mikrobiologi sesuai dengan standar SNI, dan rata-rata rendemen udang 82,54%. Nilai rata-rata produktifitas tenaga kerja di PT.XXX Pontianak-Kalimantan Barat yaitu 16,22 kg/jam/orang. Kata Kunci: Produk olahan, Udang PND, udang vaname ABSTRACT White shrimp (Litopenaeus vannamei) is a shrimp that is currently being developed in Indonesia. Frozen shrimp products are products that are favored by Indonesian people. The aims of this study were to determined quality of raw materials; cold chain application of PND processing; quality of PND shrimps; yield of product and labor productivity at PT.Pulau Mas Khatulistiwa. Raw material of shrimps were still classified as fresh shrimps (the value of organeoleptic test was 7,6). Microbial testing showed that raw shrimps microbial still fulfill SNI requirements. No antibiotik (chloramphenicol, nitrofuran, tetracyclin) were found in raw materials. Raw PND shrimps had an organoleptic value of 8. Microbiology testing of PND shrimps stil fulfill SNI requirements. Yield of PND was 82,54% and labor productivity was 16,22 kg/hour/labor at PT. XXX Pontianak-West Kalimantan. Keywords: Processed Product, Raw PND, Vaname shrimps

PROCESS INSTRUCTIONS, AS PART OF A PRODUCT QUALITY MANAGEMENT SYSTEM

Improvement of technical documentation for products from aquatic biological resources is of particular relevance and importance within the framework of creating a quality management system for fish products. In this regard, the updating and harmonization of technological instructions for the production of frozen shrimp and frozen seaweed with the modern requirements of technical regulations for production processes, ensuring the safety and high quality of finished products. The article presents technological schemes for the production of frozen shrimp and frozen seaweed, points of control of technological processes, recommended measuring instruments for controlled parameters of technological processes. Developed on the basis of an analysis of modern requirements for the production processes of frozen shrimp and frozen seaweed, the technological equipment used for their processing, the characteristics of raw materials, standard technological instructions will ensure the production of safe high quality products and can serve to form a food quality management system at fish processing enterprises.

Controlling Vaname Shrimp (Litopenaeus Vannamei) Raw Material Inventories (Case Study at PT. Grahamakmur Ciptapratama Sidoarjo)

Raw material inventory control is used to place orders and ensure that shrimp raw materials arrive in a timely manner in accordance with consumer demand. This will expedite the course of the production process. This research was conducted at PT. Grahamakmur Ciptapratama Sidoarjo with the aim of knowing and analyzing the control of raw red ginger in frozen shrimp products. The method used is the Economic Order Quantity (EOQ) Method with the Lot For Lot (LFL) Technique. In addition, safety stock analysis, Maximum Inventory, warehouse capacity and Reorder Point analysis were carried out. The results of the study showed that inventory costs using the EOQ technique were lower than the company method or using the LFL technique. The EOQ technique produces an inventory cost of IDR 292,591.00 in 2017 with an order frequency of 105 times and in 2018 of IDR 289,750.00 with a booking frequency of 116 times. In the LFL technique inventory costs are more expensive, but the planning technique in the LFL method can be applied by companies in anticipation of raw material inventory if there is a small inventory. If the company applies the EOQ method, the company purchases raw materials in a larger amount, but the warehouse capacity in the company can still be met, this can be seen from the calculation of Maximum Inventory and warehouse capacity.

Multi-Criteria Decision-Making Methods in Fuzzy Decision Problems: A Case Study in the Frozen Shrimp Industry

The European Union (EU) is the largest shrimp consumer market in the world in terms of requirements for shrimp product imports. Therefore, other enterprises that export frozen shrimp to the EU must consider many criteria when choosing suppliers of raw shrimp. The difficulty of choosing suppliers of raw shrimp makes selecting raw material suppliers in the fisheries sector a multi-criteria decision-making problem. In such problems, the decision makers must review and evaluate many criteria—including qualitative and quantitative factors—to achieve an optimal result. While there have been multiple multi-criteria decision making models developed to support supplier selection processes in different industries, none of these have been developed to solve the particular problems facing the shrimp industry, especially as it concerns a fuzzy decision-making environment. In this research, the authors propose a Multi-Criteria Decision Making model (MCDM) including the Fuzzy Analytical Network Process (FANP) and Weighted Aggregated Sum Product Assessment (WASPAS) for the evaluation and selection process of shrimp suppliers in the fisheries industry. The model is applied to a real-world case study and the results show that Supplier 3 (SA3) is the most optimal supplier of raw shrimp. The contribution of this work is the employment of FANP and WASPAS to propose an MCDM for ranking potential suppliers in the fisheries industry in a fuzzy environment. The proposed approach can also be modified to support complex decision-making processes in fuzzy environments in different industries.

Seafood Waste Management Status in Bangladesh and Potential for Silage Production

Frozen shrimp and fish are the second most valuable export items from Bangladesh. Thus, in processing industries, a considerable amount of seafood waste is produced every year. Neglecting seafood waste leads to serious forms of wastage. The purpose of this survey-based study was to estimate the amount of seafood waste produced and understand the existing waste management practices in Bangladesh. Potential for seafood waste-based silage production and its utilization were also studied. Across the seafood industry, around 43,321 tons of seafood waste are produced every year. The highest amount of seafood waste is produced in Khulna, followed by Chittagong, Cox’s Bazar, Dhaka, and Sylhet. Local people consume a portion of fresh shrimp carapace and heads and gills of large fish. A portion of seafood waste is also used to feed aquaculture species. Moreover, parts of dried shrimp shells, appendages, and fish scales, air bladders, and fins are exported to some Asian countries. The prospect of fish silage production constitutes a promising new development for animal feed production in Bangladesh. The availability of waste materials from seafood processors and the demand from feed millers favor the conditions for silage production. However, in order for the seafood waste-based silage industry to flourish, the establishment of supply chains for seafood waste and end products (silage) is required. Studies on growth performance, muscle quality, and digestibility of animal feed with silage-based diets are required for farmed species.