Efficient NIR-I fluorescence photoswitching based on a giant fluorescence quenching in photochromic nanoparticles

A photoswitchable near-infrared (NIR) fluorescent nanoparticle (NP) was designed and prepared. The NP showed a characteristic AIE property and a high-contrast NIR fluorescence photoswitching with full reversibility. Such efficient NIR...

Circumventing the optical diffraction limit with customized speckles

Speckle patterns have been widely used in imaging techniques such as ghost imaging, dynamic speckle illumination microscopy, structured illumination microscopy, and photoacoustic fluctuation imaging. Recent advances in the ability to control the statistical properties of speckles has enabled the customization of speckle patterns for specific imaging applications. In this work, we design and create special speckle patterns for parallelized nonlinear pattern-illumination microscopy based on fluorescence photoswitching. We present a proof-of-principle experimental demonstration where we obtain a spatial resolution three times higher than the diffraction limit of the illumination optics in our setup. Furthermore, we show that tailored speckles vastly outperform standard speckles. Our work establishes that customized speckles are a potent tool in parallelized super-resolution microscopy.

The first example of “turn-off” red fluorescence photoswitching for the representatives of nitrile-rich negative photochromes

The first example of reversible fluorescence photoswitching by visible light was shown for the representatives of negative photochromes containing a nitrile-rich acceptor.

Efficient “turn-off” fluorescence photoswitching in a highly fluorescent diarylethene single crystal

Efficient “turn-off” fluorescence photoswitching with full reversibility was successfully demonstrated in a fluorescent diarylethene single crystal.