Multicolor Fluorescence Photoswitching: Color-Correlated versus Color-Specific Switching

2018 ◽  
Vol 6 (20) ◽  
pp. 1800678 ◽  
Author(s):  
Dojin Kim ◽  
Soo Young Park
Keyword(s):  
Versus Color ◽  
Multicolor Fluorescence ◽  
Fluorescence Photoswitching

scholarly journals Blue-conversion of Organic Dyes Produces Artifacts in Multicolor Fluorescence Imaging

2021 ◽  
Author(s):  
Do-Hyeon Kim ◽  
Yeonho Chang ◽  
Soyeon Park ◽  
Min Gyu Jeong ◽  
Yonghoon Kwon ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Image Artifacts ◽  
Multicolor Fluorescence

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit blue-conversion phenomenon, which can produce severe multicolor image artifacts...

Eliminating Nonspecific Binding Sites for Highly Reliable Immunoassay via Super-resolution Multicolor Fluorescence Colocalization

Nanoscale ◽  
2021 ◽  
Author(s):  
Shenfei Zong ◽  
Yun Liu ◽  
Kuo Yang ◽  
Zhaoyan Yang ◽  
Zhuyuan Wang ◽  
...  
Keyword(s):  
Binding Sites ◽  
Super Resolution ◽  
Specific Adsorption ◽  
Nonspecific Binding ◽  
Nonspecific Adsorption ◽  
Multicolor Fluorescence

Non-specific adsorption in immunoassays has always been a major problem that affects the reliability of assay results. Despite the emergence of various methods which can reduce nonspecific adsorption, a universal...

Dress versus Color

1892 ◽  
Vol 20 (2) ◽  
pp. 64
Author(s):  
Henri Kelly
Keyword(s):  
Versus Color

A Locked Nucleic Acid-Based Nanocrawler: Designed and Reversible Movement Detected by Multicolor Fluorescence

2013 ◽  
Vol 135 (7) ◽  
pp. 2423-2426 ◽  
Author(s):  
I. Kira Astakhova ◽  
Karol Pasternak ◽  
Meghan A. Campbell ◽  
Pankaj Gupta ◽  
Jesper Wengel
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Multicolor Fluorescence

DIAGNOSTIC ACCURACY OF GRAY SCALE ULTRASONOGRAPHY VERSUS COLOR DOPPLER IN SUSPECTED CASES OF ACUTE APPENDICITIS

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Tahir Iqbal ◽  
Muhammad Usman Shahid ◽  
Ishfaq Ahmad Shad ◽  
Shahzad Karim Bhatti ◽  
Syed Amir Gilani ◽  
...  
Keyword(s):  
Acute Appendicitis ◽  
Positive Predictive Value ◽  
Diagnostic Accuracy ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Color Doppler ◽  
Diagnostic Tools ◽  
Gray Scale ◽  
Versus Color ◽  
Sensitivity Specificity

ABSTRACT: BACKGROUND: A common surgical emergency is acute appendicitis. Various diagnostic tools are available to diagnosis acute appendicitis. Radiological investigations play an important role in making accurate and early diagnosis and thus preventing morbidity associated with the disease. OBJECTIVE: To determine the diagnostic accuracy of gray scale ultrasonography versus color Doppler in suspected cases of acute appendicitis. MATERIALS AND METHODS: The study was carried in the department of Radiology of Mayo Hospital, Lahore. A total of 75 patients were enrolled of age 18-40 years, both genders who were suspected cases of acute appendicitis. All patients underwent baseline investigations along with gray scale ultrasonography and color Doppler. All patients were subjected to surgery to confirm the diagnosis and findings were subjected to statistical analysis. RESULTS: The mean age of the patients was 23.25 ±10.55 and mean transverse diameter of appendix was 8.37 ±3.39. There were 62.7% males and 37.3%females. Findings of gray scale ultrasonography and color Doppler were then correlated with surgical findings to calculate the diagnostic accuracy of these modalities. The results revealed that gray scale ultrasonography sensitivity, specificity, positive predictive value, negative predictive value and accuracy was 92.7%, 94.32%, 95%, 91.4% and 93.3% respectively, whereas color Doppler had sensitivity, specificity, positive predictive value, negative predictive value and accuracy of 97.7%, 93.9%, 95.3%, 97% and 96% respectively. Diagnostic accuracy of both modalities together was 98.6%. CONCLUSION: Color Doppler has better diagnostic accuracy than gray scale ultrasonography for diagnosis of acute appendicitis and the combination of both modalities yields diagnostic accuracy that is similar to gold standard.

scholarly journals Snapshot multicolor fluorescence imaging using double multiplexing of excitation and emission on a single detector

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karolina Dorozynska ◽  
Simon Ek ◽  
Vassily Kornienko ◽  
David Andersson ◽  
Alexandra Andersson ◽  
...  
Keyword(s):  
Multispectral Imaging ◽  
Fast Imaging ◽  
Imaging Method ◽  
Emission Characteristics ◽  
Cross Term ◽  
Current Implementation ◽  
Visible Wavelength Range ◽  
Imaging Approach ◽  
Single Detector ◽  
Multicolor Fluorescence

AbstractFluorescence-based multispectral imaging of rapidly moving or dynamic samples requires both fast two-dimensional data acquisition as well as sufficient spectral sensitivity for species separation. As the number of fluorophores in the experiment increases, meeting both these requirements becomes technically challenging. Although several solutions for fast imaging of multiple fluorophores exist, they all have one main restriction; they rely solely on spectrally resolving either the excitation- or the emission characteristics of the fluorophores. This inability directly limits how many fluorophores existing methods can simultaneously distinguish. Here we present a snapshot multispectral imaging approach that not only senses the excitation and emission characteristics of the probed fluorophores but also all cross term combinations of excitation and emission. To the best of the authors’ knowledge, this is the only snapshot multispectral imaging method that has this ability, allowing us to even sense and differentiate between light of equal wavelengths emitted from the same fluorescing species but where the signal components stem from different excitation sources. The current implementation of the technique allows us to simultaneously gather 24 different spectral images on a single detector, from which we demonstrate the ability to visualize and distinguish up to nine fluorophores within the visible wavelength range.

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