photoactivatable proteins
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2019 ◽  
Author(s):  
Payel Mondal ◽  
Vishnu V. Krishnamurthy ◽  
Savanna R. Sharum ◽  
Neeka Haack ◽  
Kai Zhang

AbstractNon-neuronal optogenetic approaches empower precise regulation of protein dynamics in live cells but often require target-specific protein engineering. To address this challenge, we developed a generalizable light modulated protein stabilization system (GLIMPSe) to control intracellular protein level independent of its functionality. We applied GLIMPSe to control two distinct classes of proteins: mitogen-activated protein kinase phosphatase 3 (MKP3), a negative regulator of the extracellular signal-regulated kinase (ERK) pathway, as well as a constitutively active form of MEK (CA MEK), a positive regulator of the same pathway. Kinetics study showed that light-induced protein stabilization could be achieved within 1 minute of blue light stimulation. GLIMPSe enables target-independent optogenetic control of protein activities and therefore minimizes the systematic variation embedded within different photoactivatable proteins. Overall, GLIMPSe promises to achieve light-mediated post-translational stabilization of a wide array of target proteins in live cells.


IUCrJ ◽  
2018 ◽  
Vol 5 (6) ◽  
pp. 667-672 ◽  
Author(s):  
Inokentijs Josts ◽  
Stephan Niebling ◽  
Yunyun Gao ◽  
Matteo Levantino ◽  
Henning Tidow ◽  
...  

This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged.


2016 ◽  
Vol 7 (3) ◽  
pp. 1891-1895 ◽  
Author(s):  
Shan Tang ◽  
Zhengpeng Wan ◽  
Yiren Gao ◽  
Ji-Shen Zheng ◽  
Jing Wang ◽  
...  

We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen–antibody interaction mediated responses in B cells.


Microscopy ◽  
2014 ◽  
Vol 63 (3) ◽  
pp. 255-260 ◽  
Author(s):  
Nobukazu Araki ◽  
Yuka Ikeda ◽  
Takuma Kato ◽  
Katsuhisa Kawai ◽  
Youhei Egami ◽  
...  

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