The segregation of three characters not previously reported in lettuce and loci involved in anthocyanin production were observed to segregate in crosses within and between the closely related Lactuca species L. saliva L., L. serriola L., and L. aculeata Fisch. & Mey. as well as in crosses with the more distantly related L. saligna L. Pollen color segregated 3 yellow to 1 white. Basal branching was controlled by two loci with epistasis. One of the loci was linked to a leaf-lobing locus. Anthocyanin production in several crosses seemed to be controlled by three loci rather than two loci as previously reported. The distinctive pappus bristle type of L. saligna (one row of vertical cells) was recessive to the bristle type of the other three species (two rows of vertical cells).