Metaphase spread of mouse oocytes

this protocol briefly described the procedure of performing metaphase spreads on single mouse MII (metaphase-II) oocytes. This will allow the user to count the chromosomes as well as perform additional immunofluorescence based stains for protein localization on fixed samples. the assay is however prone to false positive whole chromosome aneuploidies due to the nature of dropping the oocytes onto the etched glass slides. Hence, some skill development by repetition is required to ensure consistent outcomes. The whole procedure from obtaining oocytes to imaging happens over two days.

Distribution of sex chromosomes (XY) in lymphocyte metaphase spreads of dairy bulls

Position of autosome and sex chromosomes in metaphase spreads is grate concerned of Cytogeneticians worldwide to understand cell biology. A few isolated studies have been conducted for the distribution of chromosomes in metaphase spread. Our studies reveal that most sex chromosomes (XY) remain on periphery and semi-periphery, 84.16% for X and 86.97% for Y respectively, in round metaphase spreads. The application of sex chromosome position in metaphase spreads is to easily find out sex chromosomes under microscope even without banding patterns. An another application is to identify or confirm sex chromosomes in unknown species on which cytogenetic studies have not been performed.

Characterization of Small Supernumerary Marker Chromosomes By A Simple Molecular and Molecular Cytogenetics Approach

Characterization of Small Supernumerary Marker Chromosomes By A Simple Molecular and Molecular Cytogenetics ApproachSmall supernumerary marker chromosomes (sSMC) are still a major problem especially in prenatal cytogenetic diagnostics and counseling. These structurally abnormal chromosomes cannot be identified or characterized unambiguously by conventional banding cytogenetics alone, and are generally about the size of or smaller than a chromosome 20 in the same metaphase spread. We describe a straightforward algorithm, based on data from 2,211 reported cases (http://www.markerchromosomes.ag.vu) to quickly characterize the sSMC's chromosomal origin.