scholarly journals Metaphase Spread

2020 ◽  
Author(s):  
Keyword(s):  
Metaphase Spread

A case with dicentric translocation between chromosome 9 and 18: Confirmation by fluorescent in situ hybridization on metaphase spread

1995 ◽  
Vol 37 (1) ◽  
pp. 101-104
Author(s):  
KEIICHI ISOYAMA ◽  
MITSURU IMAI ◽  
AKIRA ISHIKAWA ◽  
KAZUHIRO FUJITA ◽  
YUMIKO SUTO ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosome 9 ◽  
Metaphase Spread

scholarly journals Metaphase spread of mouse oocytes

2020 ◽  
Author(s):  
ADITYA SANKAR
Keyword(s):  
False Positive ◽  
Protein Localization ◽  
Skill Development ◽  
Metaphase Spread ◽  
Mouse Oocytes ◽  
Glass Slides ◽  
Metaphase Ii Oocytes

Abstract this protocol briefly described the procedure of performing metaphase spreads on single mouse MII (metaphase-II) oocytes. This will allow the user to count the chromosomes as well as perform additional immunofluorescence based stains for protein localization on fixed samples. the assay is however prone to false positive whole chromosome aneuploidies due to the nature of dropping the oocytes onto the etched glass slides. Hence, some skill development by repetition is required to ensure consistent outcomes. The whole procedure from obtaining oocytes to imaging happens over two days.

scholarly journals Characterization of Small Supernumerary Marker Chromosomes By A Simple Molecular and Molecular Cytogenetics Approach

2007 ◽  
Vol 10 (1) ◽  
pp. 33-37 ◽  
Author(s):  
T Liehr ◽  
V Trifonov ◽  
A Polityko ◽  
L Brecevic ◽  
K Mrasek ◽  
...  
Keyword(s):  
Molecular Cytogenetics ◽  
Metaphase Spread ◽  
Marker Chromosomes ◽  
Chromosomal Origin ◽  
Chromosome 20 ◽  
Small Supernumerary Marker Chromosomes ◽  
Supernumerary Marker Chromosomes

Characterization of Small Supernumerary Marker Chromosomes By A Simple Molecular and Molecular Cytogenetics ApproachSmall supernumerary marker chromosomes (sSMC) are still a major problem especially in prenatal cytogenetic diagnostics and counseling. These structurally abnormal chromosomes cannot be identified or characterized unambiguously by conventional banding cytogenetics alone, and are generally about the size of or smaller than a chromosome 20 in the same metaphase spread. We describe a straightforward algorithm, based on data from 2,211 reported cases (http://www.markerchromosomes.ag.vu) to quickly characterize the sSMC's chromosomal origin.

scholarly journals Distribution of sex chromosomes (XY) in lymphocyte metaphase spreads of dairy bulls

Genetika ◽  
2013 ◽  
Vol 45 (3) ◽  
pp. 761-768
Author(s):  
Rosaiah Kotikalapudi ◽  
Rajesh Patel ◽  
Hemanth Medidi ◽  
Nagaraju Sugali
Keyword(s):  
Sex Chromosomes ◽  
Cell Biology ◽  
Sex Chromosome ◽  
Metaphase Spread ◽  
Banding Patterns ◽  
Unknown Species ◽  
Chromosome Position ◽  
Cytogenetic Studies ◽  
Dairy Bulls

Position of autosome and sex chromosomes in metaphase spreads is grate concerned of Cytogeneticians worldwide to understand cell biology. A few isolated studies have been conducted for the distribution of chromosomes in metaphase spread. Our studies reveal that most sex chromosomes (XY) remain on periphery and semi-periphery, 84.16% for X and 86.97% for Y respectively, in round metaphase spreads. The application of sex chromosome position in metaphase spreads is to easily find out sex chromosomes under microscope even without banding patterns. An another application is to identify or confirm sex chromosomes in unknown species on which cytogenetic studies have not been performed.

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