Effects of Long Noncoding RNA TUG1 (Taurine Up-Regulated Gene 1) on Growth and Metastasis of the Non-Small Cell Lung Cancer and Its Mechanism

Objective: Our research was to discuss effects and mechanism of lncRNA TUG1 in NSCLC by vitro study. Methods: A549 and H1299 cells were divided into NC, pcDNA 3.1 and lncRNA TUG1 groups. Measuring cell proliferation using CCK-8 assay, cell apoptosis by flow cytometry, invasion cell number by transwell and wound healing rate by wound healing assay. Relative gene and protein expressions by RT-qPCR and WB assay. Results: Compared with NC group, the cell proliferation rate, invasion cell number and wound healing rate were significantly depressed in A549 and H1299 cell lines (P < 0.001, respectively). By RT-qPCR and WB assay, lncRNA TUG1 gene expression were significantly increased (P < 0.001, respectively); E-cadherin gene and protein expression were significantly up-regulation, and N-cadherin and Vimentin gene and protein expressions were significantly depressed compared with those of NC group in A549 and H1299 cell lines (P < 0.001, respectively). Conclusion: lncRNA TUG1 had effects to suppress NSCLC cell biological activities by regulation EMT relative gene and proteins expression in vivo study.

Effect of Thymosin β4 on Deep Second-Degree Scald Wound Healing in Rats via Wnt/β-Catenin Pathway

<sec> <title>Objective:</title> The purpose of this research is to explore the influences of thymosin β4 (Tβ4) in deepsecond-degree scald wound healing of rat skin and its relationship with Wnt/β-catenin pathway. </sec> <sec> <title>Methods:</title> Deep second-degree scalded model rats were prepared and divided into normal saline (NS) treatment group, Tβ4 treatment group and FH535 inhibitor group. Then, the concentrations of inflammatory factors in the rats were monitored via adopting the correlated TNF-α and IL-1β ELISA kits. In the meantime, the wound healing rate was analyzed via photography. Subsequently, the qRTPCR procedure was wielded to determine Wnt1 and β-catenin expression in wound tissues, and the degree of wound tissue injury was examined via hematoxylin and eosin (HE) staining. Finally, Western blotting (WB) was adopted to assess Wnt/β-catenin pathway-associated protein levels. </sec> <sec> <title>Results:</title> Releasing amount of TNF-α and IL-1β were conspicuously up-regulated after scalding (p <0.01), and Wnt1 and β-catenin expression at molecular transcription level was also significantly raised (p < 0.01). Besides, treatment with 18 μg of Tβ4 significantly increased the wound healing rate of scalded rats (p < 0.01). In addition, Tβ4 treatment significantly promoted wound healing (p < 0.01) and increased the Wnt1 and β-catenin expression levels (p < 0.01). Moreover, FH535 significantly restrained the Wnt/β-catenin pathway-correlated protein levels (p < 0.01) and wound healing. </sec> <sec> <title>Conclusion:</title> Tβ4 can promote scald wound healing in rats and may play a role via evoking Wnt/β-catenin pathway activation. </sec>

Nanosilver Dressing in Treating Deep II Degree Burn Wound Infection in Patients with Clinical Studies

Background. Patients’ clinical antibiotic treatment of deep II degree burns usually fails to achieve the ideal effect; in order to avoid the late result in pigmentation, scarring, and even limb dysfunction, it also needs to deal effectively with burn wounds. Aim. The purpose of this study is to evaluate nanosilver dressing in treating deep II degree burn wound infection in patients with clinical studies. Materials and Methods. 106 burn patients were classified into the Sulfadiazine Silver Cream (SSC) group ( n = 53 ) and the Nanosilver Burn Dressing (NSBD) group ( n = 53 ). Both of them received basic wound treatment, and wound healing time and pigmentation fading away time of all patients were recorded. And the wound healing rate of the patients was calculated. Serum levels of tumor necrosis factor alpha (TNF-α) and interleukin-1beta (IL-1β) were detected pre- and posttreatment. Results. After basic treatment for all patients, Sulfadiazine Silver Cream was used in the SSC group, and Nanosilver Burn Dressing was used in the NSBD group. It was observed that after treatment, compared with the SSC group, there was significant efficiency; wound healing rate, healing time, and pigmentation fading away time were shortened in the NSBD group, and IL-1β levels were decreased, and the positive rate of bacterial culture was decreased (all P < 0.05 ). Conclusion. Nanosilver Burn Dressing in treating deep II degree burns can effectively reduce the wound infection and promote wound healing. The curative effect was distinct, which was worthy of popularization and application.

Curative Effect Observation of Applying Silver-Zinc Bacteriostatic Cream to Patients with Second-Degree Burn under Targeted Nursing Intervention and Its Effect on Wound Healing Rate

Second-degree burn is the most common type of burn wound in the clinics, which presents a deeper wound, skin blisters, white or red bottom, and thick and clear fluid inside, is sensitive to tenderness, and turns white by compression [1, 2]. The aim of this study is to explore the efficacy of applying silver-zinc bacteriostatic cream to patients with second-degree burn under targeted nursing intervention and its effect on wound healing rate. A total of 110 patients with second-degree burn treated in our hospital from January 2019 to June 2021 were selected as the research object for the retrospective study. Between the experimental group and the control group, no statistical differences in patients’ general information were observed ( P > 0.05 ); 1 week, 2 weeks, and 3 weeks after treatment, the visual analogue scale (VAS) pain scores of the experimental group were significantly lower ( P < 0.05 ); and the total incidence rate of adverse reactions was remarkably lower in the experimental group ( P < 0.05 ). On the basis of targeted nursing intervention, applying silver-zinc bacteriostatic cream obtains an obviously better clinical efficacy than silver sulfadiazine ointment in treating second-degree burn and works better in promoting wound healing, relieving pain sensation, and reducing adverse reactions.

Effect of Combining Immersion Therapy with Shengji Ointment on Wound Healing Rate and Adverse Reaction Rate in Patients with Second-Degree Burn

Objective. To explore the effect of combining immersion therapy with Shengji ointment on wound healing rate and adverse reaction rate (ARR) in patients with second-degree burn. Methods. The data of 80 patients with second-degree burn treated in our hospital from February 2019 to February 2020 were retrospectively analyzed by the means of retrospective study, and the patients were equally divided into the treatment group and the control group according to their admission order, with 40 cases each. Immersion therapy was performed to all patients (7 d); after that, patients in the control group received routine medication (7 d), and those in the treatment group were treated with Shengji ointment until the wounds were healed, so as to compare their wound healing condition, ARRs, levels of inflammatory factors, and infection incidence. Results. Compared with the control group after treatment, the treatment group presented significantly shorter wound healing time (12.14 ± 1.26 vs. 15.98 ± 1.20, P < 0.001), better wound healing rate and quality ( P < 0.05), and lower levels of inflammatory factors ( P < 0.001); no significant between-group difference in ARRs was shown ( P > 0.05); 34 patients in the treatment group (85.0%) and 26 patients in the control group (65.0%) had no infections, so the incidence rate of infections was significantly lower in the treatment group than in the control group (P < 0.05). Conclusion. Combining immersion therapy with Shengji ointment can reduce the levels of inflammatory factors in patients with second-degree burn, lower the incidence rate of infections, provide the conditions for wound healing, and increase the wound healing rate, which shall be promoted and applied in practice.

Study on the mechanism of microporous sheep ADM combined with hUCMSC in promoting wound healing

Objective: To explore the therapeutic mechanism of microporous sheep acellular dermal matrix (ADM) combined with human umbilical cord mesenchymal stem cell (hUCMSC) to promote the healing of full-thickness skin defect.Methods: hUCMSC was co-cultured on microporous sheep ADM to form composite biological dressings. Seventy-two nude mice were selected to make full-thickness skin injury models and randomly divided into 3 groups (hUCMSC + microporous sheep ADM group, sheep ADM group and iodophor gauze group), with 24 mice in each group. The wound healing rate of each group was detected at 14 d, 21 d and 28 d after operation, qRT-PCR technique was used to detect the expression of Bax and Bcl-2 in the wound tissues, and the immunohistochemical staining technique was used to detect the expression of Collagen I and vascular endothelial growth factor (VEGF). Data were analyzed with one-way ANOVA and t test.Results: At 14 d after operation, the wound healing rate of the hUCMSC + microporous sheep ADM group was (65.34 ± 14.72)%, which was significantly higher than that of the iodophor gauze group [(37.54 ± 10.21)%], and higher than that of the sheep ADM group [(49.08 ± 11.16)%], the differences were statistically significant (t = 19.52, 14.72; p < .05). With the gradual healing of the wound, at 28 d after operation, the wound healing rate of the hUCMSC + microporous sheep ADM group was (98.63 ± 15.41)%, which was significantly higher than that of the iodophor gauze group [(81.74 ± 16.27)%], and higher than that of the sheep ADM group [(63.47 ± 14.80)%], the differences were statistically significant (t = -16.42, 20.35; p < .05). The expression of Bax in the wound tissues of the hUCMSC + microporous sheep ADM group was significantly reduced, especially at 21 d after operation, the expression level was 0.25 ± 0.06, which was significantly lower than the iodophor gauze group (0.53 ± 0.16) and the sheep ADM group (0.41 ± 0.12), the differences were statistically significant (t = 3.52, -2.83; p < .05). The expression of Bcl-2 in the wound tissues of the hUCMSC + microporous sheep ADM group was significantly higher than those ofthe other two groups, especially at 21 d after operation, the expression level was 0.63 ± 0.19, which was significantly higher than the iodophor gauze group (0.34 ± 0.09) and the sheep ADM group (0.46 ± 0.13), the differences were statistically significant (t = 5.31, -6.07; p < .05). Immunohistochemical detection showed that the expression of Collagen I and VEGF in the hUCMSC + microporous sheep ADM group was slightly more than that of the sheep ADM group and the iodophor gauze group, but the effect was not remarkable.Conclusions: hUCMSC + microporous sheep ADM composite dressing can promote the healing of the full-thickness skin injury and reduce the production of apoptotic cells by carrying hUCMSC.

Therapeutic laser with or without andiroba oil in treating cutaneous wounds by second intention in Wistar rats

This study’s objective was to evaluate the efficacy of treating cutaneous wounds in Wistar rats using a therapeutic laser alone or in combination with topical application of andiroba oil. Twenty-four Wistar rats were distributed into three groups (T4, T7, and T14) of eight animals. To prepare the wounds, a total of four skin fragments per animal were removed using an 8-mm cutaneous biopsy punch. Each animal was inflicted with four surgical wounds, and each wound was subjected to one treatment. The treatments were as follows: saline solution (control, Cn); laser therapy (L), using a 660-nm laser wavelength and 10-J/cm² energy density; fresh andiroba oil (An); laser therapy followed by topical andiroba oil administration (LAn). All treatments in all animals were conducted for 4- (T4), 7- (T7), and 14- (T14) day periods. Edema and purulent secretion were observed in three animals in the An group, and the appearance of an exuberant crust was also observed in one animal from the same group. The LAn group presented the worst wound healing rate and contraction velocity (p < 0.05). Microscopically, there was no difference between groups regarding the presence of inflammation, necrosis, formation of granulation tissue, fibroplasia, and the presence of types 1 and 3 collagen at different treatment times. It was concluded that laser treatment of cutaneous wounds in conjunction with andiroba oil application did not present benefits in reference to the 0.9% NaCl.

Efficacy of Oxidized Regenerated Cellulose/Collagen Dressing for Management of Skin Wounds: A Systematic Review and Meta-Analysis

Objective. The purpose of this study was to evaluate the wound healing efficacy of oxidized regenerated cellulose (ORC)/collagen dressing and ORC/collagen/silver-ORC dressings compared to standard of care or control in treatment of chronic skin wounds such as diabetic foot ulcers (DFUs), venous leg ulcers (VLUs), and pressure injuries sore ulcers (PISUs). Methods. An electronic search was carried out in four popular databases PubMed, Scopus, Embase, and CENTRAL to identify thirteen included studies, comparing the clinical efficacy of ORC/collagen dressings when compared to control in management of chronic skin wounds, especially DFUs, VLUs, and PISUs, and skin graft donor site wounds. Results. Consolidated data from thirteen comparative clinical studies undertaken for management of DFUs, VLUs, and PISUs showed favorable outcomes towards use of ORC/collagen compared to other traditional and hydrocolloid foam dressings in terms of wound healing rate ( P = 0.02 ) and percentage wound relative reduction ( P = 0.003 ). The time taken to achieve complete wound healing in the included studies did not show any statistical significant difference ( P = 0.24 ). There was no significant difference in adverse events between ORC/collagen-treated group and comparative group ( P = 0.19 ). Conclusion. ORC/collagen wound dressings are beneficial in terms of improved wound healing rate and percentage wound relative reduction compared to already existing traditional standard of care with non-MMP, inhibiting biomaterials such as moistened gauze, autologous growth factors, hydrocolloid foam dressings, or ovine extracellular matrix.

Skin Wound Healing Rate in Fish Depends on Species and Microbiota

The skin is a barrier between the body and the environment that protects the integrity of the body and houses a vast microbiota. By interacting with the host immune system, the microbiota improves wound healing in mammals. However, in fish, the evidence of the role of microbiota and the type of species on wound healing is scarce. We aimed to examine the wound healing rate in various fish species and evaluate the effect of antibiotics on the wound healing process. The wound healing rate was much faster in two of the seven fish species selected based on habitat and skin types. We also demonstrated that the composition of the microbiome plays a role in the wound healing rate. After antibiotic treatment, the wound healing rate improved in one species. Through 16S rRNA sequencing, we identified microbiome correlates of varying responses on wound healing after antibiotic treatment. These findings indicate that not only the species difference but also the microbiota play a significant role in wound healing in fish.

A comparative multicentre study evaluating gluteal turnover flap for wound closure after abdominoperineal resection for rectal cancer

Background The aim of this study was to compare perineal wound healing between gluteal turnover flap and primary closure in patients undergoing abdominoperineal resection (APR) for rectal cancer. Methods Patients who underwent APR for primary or recurrent rectal cancer with gluteal turnover flap in two university hospitals (2016–2021) were compared to a multicentre cohort of primary closure (2000–2017). The primary endpoint was uncomplicated perineal wound healing within 30 days. Secondary endpoints were long-term wound healing, related re-interventions, and perineal herniation. The perineal hernia rate was assessed using Kaplan Meier analysis. Results Twenty–five patients had a gluteal turnover flap and 194 had primary closure. The uncomplicated perineal wound-healing rate within 30 days was 68% (17/25) after gluteal turnover flap versus 64% (124/194) after primary closure, OR 2.246; 95% CI 0.734–6.876; p = 0.156 in multivariable analysis. No major wound complications requiring surgical re-intervention occurred after flap closure. Eighteen patients with gluteal turnover flap completed 12-month follow-up, and none of them had chronic perineal sinus, compared to 6% (11/173) after primary closure (p = 0.604). The symptomatic 18-month perineal hernia rate after flap closure was 0%, compared to 9% after primary closure (p = 0.184). Conclusions The uncomplicated perineal wound-healing rate after the gluteal turnover flap and primary closure after APR is similar, and no chronic perineal sinus or perineal hernia occurred after flap closure. Future studies have to confirm potential benefits of the gluteal turnover flap.