Aspects of the Process of Sorting European Black Pine Seeds

Research Highlights: Seed separation criteria and the optimal parameters of sorting devices were described. Background and Objectives: Seeds are often sorted into fractions which are sown separately to promote uniform seed germination and seedling emergence. Therefore, the aim of this study was to determine the correlations between the basic physical properties of European black pine (Pinus nigra J.F. Arnold subsp. nigra) seeds for the needs of planning seed sorting operations. Materials and Methods: Black pine seeds were divided into 5 batches representing individual parent trees, and the physical properties (terminal velocity, thickness, width, length, angle of external friction, mass) of each seed were determined. The measured geometric parameters and seed mass were used to calculate the respective indicators for each seed. The values of the analyzed parameters were used to plan the seed separation process. Results: The average values of the basic physical properties of seeds were determined in the following range: Terminal velocity—8.32 to 8.73 m s−1, thickness—2.24 to 2.27 mm, width—3.34 to 3.44 mm, length—5.87 to 6.08 mm, angle of external friction—28 to 32°, mass—18.8 to 20.0 mg. Seed mass was most highly correlated with terminal velocity, and it was least correlated with the angle of external friction. Conclusions: The results of this study indicate that black pine seeds should be sorted with the use of pneumatic separators or, alternatively, mesh sieves with longitudinal openings. These sorting devices separate seeds into fractions characterized by similar seed mass, which delivers both economic and environmental benefits in nursery practice.

The genetic structure of the European black pine (Pinus nigra Arnold) is shaped by its recent Holocene demographic history

Fragmentation acting over geological times confers wide, biogeographical scale, genetic diversity patterns to species, through demographic and natural selection processes. To test the effects of historical fragmentation on the genetic diversity and differentiation of a major European forest tree and to resolve its demographic history, we describe and model its spatial genetic structure and gene genealogy. We then test which Pleistocene event, whether recent or ancient, could explain its widespread but patchy geographic distribution using population genetic data, environmental data and realistic demographic timed scenarios.The taxon of interest is a conifer forest tree, Pinus nigra (Arnold), the European black pine, whose populations are located in the mountains of southern Europe and North Africa, most frequently at mid-elevation. We used a set of different genetic markers, both neutral and potentially adaptive, and either bi-parentally or paternally inherited, and we sampled natural populations across the entire range of the species. We analysed the data using frequentist population genetic methods as well as Bayesian inference methods to calibrate realistic, demographic timed scenarios.Species with geographically fragmented distribution areas are expected to display strong among-population genetic differentiation and low within-population genetic diversity. Contrary to these expectations, we show that the current diversity of Pinus nigra and its weak genetic spatial structure are best explained as resulting from late Pleistocene or early Holocene fragmentation of one ancestral population into seven genetic lineages, which we found to be the main biogeographical contributors of the natural black pine forests of today. Gene flow among the different lineages is strong across forests and many current populations are admixed between lineages. We propose to modify the currently accepted international nomenclature made of five subspecies and name these seven lineages using regionally accepted subspecies-level names.HighlightsThe European black pine, Pinus nigra (Arnold), has a weak spatial genetic structure.Gene flow among populations is frequent and populations are often of admixed origin.Current genealogies result from recent, late Pleistocene or Holocene events.Seven modern genetic lineages emerged from divergence and demographic contractions.These seven lineages warrant a revision of subspecies taxonomic nomenclature.

Lluciapomaresius nisae, a new species of Ephippigerini (Orthoptera: Tettigoniidae: Bradyporinae) from the northeast of the Iberian Peninsula

A new species of the genus Lluciapomaresius Barat, 2012 is described from Serra de Llaberia in Catalonia (in the northeast of Iberian Peninsula). Lluciapomaresius nisae n. sp. was collected in a Mediterranean pine forest dominated by European black pine (Pinus nigra) and secondarily by Calcicolous rosemary scrub. L. nisae is compared to L. panteli (Navàs, 1899) from which it can be separated mainly by the shape of the male cerci, the titillators and the male calling song. Also in the females by the protuberances at the base of the ventral valves of the ovipositor.