Intrinsic positional memory guides target-specific axon regeneration in the zebrafish vagus nerve

ABSTRACT Regeneration after peripheral nerve damage requires that axons re-grow to the correct target tissues in a process called target-specific regeneration. Although much is known about the mechanisms that promote axon re-growth, re-growing axons often fail to reach the correct targets, resulting in impaired nerve function. We know very little about how axons achieve target-specific regeneration, particularly in branched nerves that require distinct targeting decisions at branch points. The zebrafish vagus motor nerve is a branched nerve with a well-defined topographic organization. Here, we track regeneration of individual vagus axons after whole-nerve laser severing and find a robust capacity for target-specific, functional re-growth. We then develop a new single-cell chimera injury model for precise manipulation of axon-environment interactions and find that (1) the guidance mechanism used during regeneration is distinct from the nerve's developmental guidance mechanism, (2) target selection is specified by neurons' intrinsic memory of their position within the brain, and (3) targeting to a branch requires its pre-existing innervation. This work establishes the zebrafish vagus nerve as a tractable regeneration model and reveals the mechanistic basis of target-specific regeneration.

Program Bimbingan dan Konseling Berbasis Nilai Karakter Taluba Bagi Siswa SMA di Kota Banjarmasin

This study aims to determine the design of Guidance and counseling programs in Taluba-based schools in the development of the character of learners, to find out the Taluba Character Concepts in the Banjar / Swamp Tribe Society, and to determine the validity of the developmental guidance and counseling program model. The design of this study is the design of educational research and development (Educational Research & Development) Research and development in the context of education is the process used to develop and validate educational products. The stages used in this study were only 8 stages out of 10 stages. The subjects of development in this study were all high schools in Banjarmasin, both public and private. The approach in this study uses qualitative and quantitative approaches. The technique for collecting data is to use interview techniques and documentation in the form of a literature review and use the Delphi to validate the model created by researchers as guidance and counseling experts and practitioners. The target of this research output is to become a speaker at national/international seminars and to be published in Sinta's e-ISSN Journal. The results obtained are based on the results of quantitative and qualitative validation tests by 3 experts in BK Science and 3 Practitioners who are BK teachers/school counselors who indeed understand the science of Guidance and counseling, the six validators alike state that the hypothetical model of the BK program is developed based Taluba character values ​​are very good, useful, easy, proper and appropriate to be used as a reference for BK teachers in providing guidance and counseling services that are integrated with local wisdom and the culture of the Banjar tribe community.

Perceptions of Healthcare Clinicians on Occupational Therapists Providing Services During Well-Baby Visits

Background: To explore the perceptions of healthcare clinicians on the involvement of occupational therapists during a well-baby visit to allot for more face-to-face time with a healthcare practitioner and more efficient developmental milestone screening. Due to the high demand of healthcare clinicians, well-baby visits are becoming shorter caused by lack of availability for healthcare professionals to take on the roles of parent education. Occupational therapy practitioners are qualified as developmental specialists. Therefore, occupational therapy practitioners’ involvement in well-baby visits can compensate for this lack of time and allow for additional parent education and screening for developmental delay. Methods: A 13 question survey to quantitatively analyze the perceptions of healthcare clinicians using a Likert-type psychometric rating scale. The Qualtrics XM survey was distributed to licensed practitioners (i.e. primary care physicians, pediatricians, obstetrician-gynecologists, advanced practice clinicians) through Facebook groups and snowball sampling. Data was analyzed using the Statistical Package for the Social Sciences. Results: Majority of clinicians believed the inclusion of occupational therapy during a well-baby visit would be beneficial in providing preventative education and developmental guidance to prevent developmental delay. This research was successful in determining that gender, age, and years of experience did not influence the perceptions of healthcare clinicians in regard to the inclusion of occupational therapy practitioners in primary care. Additional findings indicated that there is a discrepancy between the needs of parents and caregivers and the perceptions of healthcare clinicians regarding the sufficiency of education and resources provided during well-baby visits. Conclusion: Majority of clinicians believed the inclusion of occupational therapy practitioners during a well-baby visit would be beneficial to provide preventative education and developmental guidance to prevent developmental delay. Gender, age, and years of experience did not influence the perceptions of healthcare clinicians in regard to the inclusion of occupational therapy practitioners in primary care. Trial Registration: Retrospectively registered

The Glial Perspective on Sleep and Circadian Rhythms

While neurons and circuits are almost unequivocally considered to be the computational units and actuators of behavior, a complete understanding of the nervous system must incorporate glial cells. Far beyond a copious but passive substrate, glial influence is inextricable from neuronal physiology, whether during developmental guidance and synaptic shaping or through the trophic support, neurotransmitter and ion homeostasis, cytokine signaling and immune function, and debris engulfment contributions that this class provides throughout an organism's life. With such essential functions, among a growing literature of nuanced roles, it follows that glia are consequential to behavior in adult animals, with novel genetic tools allowing for the investigation of these phenomena in living organisms. We discuss here the relevance of glia for maintaining circadian rhythms and also for serving functions of sleep.

Tuba1a is uniquely important for axon guidance through midline commissural structures

Developing neurons undergo dramatic morphological changes to appropriately migrate and extend axons to make synaptic connections. The microtubule cytoskeleton, made of α/β-tubulin dimers, drives neurite outgrowth, promotes neuronal growth cone responses, and facilitates intracellular transport of critical cargoes during neurodevelopment. TUBA1A constitutes the majority of α-tubulin in the developing brain and mutations to TUBA1A in humans cause severe brain malformations accompanied by varying neurological defects, collectively termed tubulinopathies. Studies of TUBA1A function in vivo have been limited by the presence of multiple genes encoding highly similar tubulin proteins, which prevents TUBA1A-specific antibody generation and makes genetic manipulation challenging. Here we present a novel tagging method for studying and manipulating TUBA1A in cells without impairing tubulin function. Using this tool, we show that a TUBA1A loss-of-function mutation TUBA1AN102D (TUBA1AND), reduced the amount of TUBA1A protein and prevented incorporation of TUBA1A into microtubule polymers. Reduced Tuba1a α-tubulin in heterozygous Tuba1aND/+ mice significantly impacted axon extension and impaired formation of forebrain commissures. Neurons with reduced Tuba1a caused by Tuba1aND had altered microtubule dynamics and slower neuron outgrowth compared to controls. Neurons deficient in Tuba1a failed to localize microtubule associated protein-1b (Map1b) to the developing growth cone, likely impacting reception of developmental guidance cues. Overall, we show that reduced Tuba1a is sufficient to support neuronal migration, but not axon guidance, and provide mechanistic insight as to how TUBA1A tunes microtubule function to support neurodevelopment.

Strategy Guidance and Counseling Comprehensive Based Spiritual Intelligence for Student in the Digital Era

The purpose of this paper is to discuss strategy guidance and counseling comprehensive based on spiritual intelligence for students in the digital age. Guidance and counseling comprehensive is part of education, which is also a model of guidance and counseling that adheres to the principle of developmental guidance and counseling. The basic assumption of the Guidance and development counseling approach is the idea that healthy individual development will occur in the healthy interactions of individuals with their environment. In other words, the environment for individuals becomes a learning environment, because there are various intelligences that need to be considered in the process of individual development and spiritual intelligence is one aspect of individual development that needs attention. Spiritual intelligence is the highest form of intelligence that combines the two previous forms of intelligence, namely intellectual intelligence and emotional intelligence. Spiritual intelligence is considered as the highest intelligence because it is closely related to people's awareness to be able to interpret everything and is a way to be able to feel happiness. Especially in the digital era, the era illustrates that the technological revolution phase has changed the way and human behavior in scale, scope, complexity, and transformation from previous life experiences, requires that every individual must have good spiritual intelligence, so that they can face the development of the digital era this dynamic well.

The Collaborative Role of Counselors in Inclusive Schools

Strengthening Inclusive Education requires the role of various parties in educational institutions including guidance and counseling. Guidance and counseling services at the Inclusive Schools currently oriented towards developmental guidance and counseling with targeted services not limited to normal students in institutional settings, but will be directed to all students in various life and cultural settings including their special needs. Inclusive education is a shared responsibility between school Counselor, Teacher, Special Assistant teacher (GPK), and also Parents. Teachers cannot do everything themselves, because parents need to be involved so that the implementation of activities in school continues with the activities of students at home. Collaboration forms the basis between school and parents, as well as other experts, to provide assistance in a systematic, programmed, consistent and ongoing manner. Thus, the collaborative role of the counselors is needed, the development of the counselor's skills, and the professional implementation of the guidance and counseling program to optimize the development of students with special needs in inclusive schools. Collaboration for student success is an integral part of educational reform in inclusive schools

Guided High-Quality Development, Resources, and Environmental Forcing in China’s Green Development

High-quality guided development and environmental forcing are two routes for China’s green development, and each has different focuses and outcomes. The aim of this study is to clarify coordinated mechanisms to reveal the reasons and determine paths for China’s green development. A research framework for synergetic evolution is established, and the Haken model is applied to analyze the different effects of high-quality development that guide resource- and environmental-forcing mechanisms. This research showed that: (1) the preferred route and key factor in China’s green development is high-quality developmental guidance; (2) high-quality development and environmental-forcing mechanisms are non-coordinated, and while the former might coordinate with the latter, the latter does not; (3) mutual promotion of high-quality developmental guidance and the resource mechanism was not observed; (4) the critical point coordinated value is 0.5686 for China’s green development. Eastern China possesses a relatively high level of green development, while the north, east, west, and central areas of the country still have much progress to make.

Comprehensive School Guidance Programs in Nebraska

Archival data from an in-state survey of 428 elementary and secondary school counselors completed by the Nebraska Department of Education regarding comprehensive guidance programs was reviewed for relevant information. This information is discussed relative to the current views and knowledge regarding the state of comprehensive developmental guidance and their implications for school counselors and administrators.