ETIOLOGY OF SKIN LEISHMANIOSIS IN OF ENDEMIC REGIONS OF UZBEKISTAN ON THE EXAMPLE OF DZHIZAK REGION

Taking into account the high prevalence of cutaneous leishmaniasis in Uzbekistan, as well as the tendency of increase in the incidence of this disease over the past 5 years, important and necessary is to determine the species composition of leishmania, as well as their vectors and natural reservoirs. According to the results of molecular-biological diagnostics, carried out in present study, in samples taken from ulcers of patients from Jizzakh region, which previously considered endemic for anthraponotic cutaneous leishmaniasis, was detected L.mayor, the causative agent of zoonotic cutaneous leishmaniasis.

CONTEMPORARY MOLECULAR-GENETIC METHODS USED FOR ETIOLOGIC DIAGNOSTICS OF SEPSIS

Etiologic diagnostics of sepsis is one of the most difficult problems of contemporary medicine due to a wide variety of sepsis causative agents, many of which are components of normal human microflora. Disadvantages of contemporary «golden standard» of microbiologic diagnostics of sepsis etiology by seeding of blood for sterility are duration of cultivation, limitation in detection of non-cultivable forms of microorganisms, significant effect of preliminary empiric antibiotics therapy on results of the analysis. Methods of molecular diagnostics that are being actively developed and integrated during the last decade are deprived of these disadvantages. Main contemporary methods of molecular-biological diagnostics are examined in the review, actual data on their diagnostic characteristic are provided. Special attention is given to methods of PCR-diagnostics, including novel Russian developments. Methods of nucleic acid hybridization and proteomic analysis are examined in comparative aspect. Evaluation of application and perspectives of development of methods of molecular diagnostics of sepsis is given.

Advanced methods for molecular-biological diagnostics of genital infections

At present to diagnose infectious diseases new achievements of molecular biology are more often used equally with classic cultural methods. The article briefly presents the base of molecular-biological diagnostics of infections and the use of nucleic acids amplification with different arrangements of polimerase chain reaction including that being performed in real time. Examples of quantitative determination of DNA of microorganisms actual for obstetrics and neonatology.