scholarly journals Advanced methods for molecular-biological diagnostics of genital infections

2003 ◽  
Vol 52 (1) ◽  
pp. 78-82
Author(s):  
М. A. Bashmakova ◽  
Alevtina M. Savicheva
Keyword(s):  
Molecular Biology ◽  
Infectious Diseases ◽  
Nucleic Acids ◽  
Quantitative Determination ◽  
Real Time ◽  
Chain Reaction ◽  
Genital Infections ◽  
Cultural Methods ◽  
Molecular Biological Diagnostics

At present to diagnose infectious diseases new achievements of molecular biology are more often used equally with classic cultural methods. The article briefly presents the base of molecular-biological diagnostics of infections and the use of nucleic acids amplification with different arrangements of polimerase chain reaction including that being performed in real time. Examples of quantitative determination of DNA of microorganisms actual for obstetrics and neonatology.

scholarly journals A one-step method for quantitative determination of uracil in DNA by real-time PCR

2010 ◽  
Vol 38 (21) ◽  
pp. e196-e196 ◽  
Author(s):  
András Horváth ◽  
Beáta G. Vértessy
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Step Method ◽  
One Step

scholarly journals Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maria Kaltenbrunner ◽  
Walter Mayer ◽  
Kirsten Kerkhoff ◽  
Rita Epp ◽  
Hermann Rüggeberg ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Wild Boar ◽  
Real Time ◽  
Real Time Pcr ◽  
Processed Food ◽  
Domestic Pig ◽  
The Real ◽  
Qualitative And Quantitative ◽  
Pcr Assays

Abstract Appropriate analytical methods are needed for the detection of food authentication. We investigated the applicability of a duplex real-time PCR assay targeting chromosome 1 and two singleplex real-time PCR assays targeting chromosome 9, both published recently, for the qualitative and quantitative determination of wild boar and domestic pig in processed food products. In addition, two singleplex real-time PCR assays targeting chromosome 7 were tested for their suitability to differentiate the two subspecies. Even by targeting the three genome loci, the probability of misclassification was not completely eliminated. Application of the real-time PCR assays to a total of 35 commercial meat products, including 22 goulash products, revealed that domestic pig DNA was frequently present, even in 14 out of 15 products declared to consist of 100% wild boar. Quantitative results obtained with the real-time PCR assays for wild boar (p < 0.001) and those for domestic pig (p < 0.001) were significantly different. However, the results obtained with the real-time PCR assays for wild boar (r = 0.673; p < 0.001) and those for domestic pig (r = 0.505; p = 0.002) were found to be significantly correlated. If the rules given in the paper are followed, the real-time PCR assays are applicable for routine analysis.

A real-time fluorescent sensor specific to Mg2+: crystallographic evidence, DFT calculation and its use for quantitative determination of magnesium in drinking water

2015 ◽  
Vol 44 (6) ◽  
pp. 2755-2762 ◽  
Author(s):  
Guangwen Men ◽  
Chunrong Chen ◽  
Shitong Zhang ◽  
Chunshuang Liang ◽  
Ying Wang ◽  
...  
Keyword(s):  
Drinking Water ◽  
Quantitative Determination ◽  
Real Time ◽  
Dft Calculation ◽  
Fluorescent Sensor ◽  
Turn On ◽  
Quantitative Monitoring ◽  
Fluorescence Turn On

An “off-the-shelf” fluorescence “turn-on” Mg2+ chemosensor BCSA has been developed for real-time quantitative monitoring of magnesium in drinking water.

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