conduction function
Recently Published Documents


TOTAL DOCUMENTS

21
(FIVE YEARS 3)

H-INDEX

4
(FIVE YEARS 0)

2021 ◽  
Vol 118 (49) ◽  
pp. e2106459118
Author(s):  
Bingxue Li ◽  
Songling Li ◽  
Honglan Zheng ◽  
Zhiqiang Yan

Auditory transduction is mediated by chordotonal (Cho) neurons in Drosophila larvae, but the molecular identity of the mechanotransduction (MET) channel is elusive. Here, we established a whole-cell recording system of Cho neurons and showed that two transient receptor potential vanilloid (TRPV) channels, Nanchung (NAN) and Inactive (IAV), are essential for MET currents in Cho neurons. NAN and IAV form active ion channels when expressed simultaneously in S2 cells. Point mutations in the pore region of NAN-IAV change the reversal potential of the MET currents. Particularly, residues 857 through 990 in the IAV carboxyl terminus regulate the kinetics of MET currents in Cho neurons. In addition, TRPN channel NompC contributes to the adaptation of auditory transduction currents independent of its ion-conduction function. These results indicate that NAN-IAV, rather than NompC, functions as essential pore-forming subunits of the native auditory transduction channel in Drosophila and provide insights into the gating mechanism of MET currents in Cho neurons.


2021 ◽  
Author(s):  
Meijian Wang ◽  
Min Xie ◽  
Shuyan Yu ◽  
Pan Shang ◽  
Cong Zhang ◽  
...  

Abstract Diabetic peripheral neuropathy (DPN) is a chronic complication of diabetes, and its exact pathogenesis remains unclear. Autophagy plays an important role in neurodegenerative diseases, ischemia–reperfusion injury of nerve tissues, and nerve tissue injury repair. Lipin1 is a phosphatidic acid phosphatase enzyme that converts phosphatidic acid (PA) into diacylglycerol (DAG), a precursor of triacylglycerol and phospholipids which plays an important role in maintaining normal peripheral nerve conduction function. Here, we show that induction of DPN rat model via STZ injection could reduce Lipin1 expression, prevent DAG synthesis, and results in autophagic hyperactivity. Interestingly, these effects increase the apoptosis of Schwann cells and lead to demyelination in sciatic nerve in DPN rats. More importantly, upregulation of lipin1 in the DPN rats ameliorated autophagy disorders and pathological changes of the sciatic nerve, which associated with the increase of the motor nerve conductive velocity (MNCV) in DPN rats. In contrast, lipin1 downregulation exacerbates neuronal abnormalities and facilitates the genesis of DPN phenotypes in rats. In addition, overexpression of lipin1 in RSC96 cells also significantly decreased the autophagic hyperactivity and apoptosis induced by hyperglycemia. These results suggest that lipin1 may exert neuroprotection within the sciatic nerve anomalies and may serve as a potential therapeutic target for the treatment of DPN.


2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Petra Huehnchen ◽  
Klaus Viktor Toyka ◽  
Karen Gertz ◽  
Matthias Endres ◽  
Wolfgang Boehmerle

Abstract Objective Critical illness polyneuropathy (CIP) is a common complication of severe systemic illness treated in intensive care medicine. Ischemic stroke leads to an acute critical injury of the brain with hemiparesis, immunosuppression and subsequent infections, all of which require extended medical treatment. Stroke-induced sarcopenia further contributes to poor rehabilitation and is characterized by muscle wasting and denervation in the paralytic, but also the unaffected limbs. Therefore, we asked whether stroke leads to an additional CIP-like neurodegeneration. Results Focal brain ischemia was induced in adult mice by 60-min middle cerebral artery occlusion (MCAo) following reperfusion and led to functional deficits and marked hemispheric brain atrophy. Nerve conduction function and muscle potentials were measured in the ipsilateral sciatic nerve and gastrocnemius and quadriceps muscle with electroneurography/-myography on days 10, 22, 44 after stroke. An additional crush-injury to the sciatic nerve was included in two sham mice as positive control (sham +). We found no differences in nerve conduction function nor spontaneous electromyographic activity between MCAo and sham animals. Sham + mice developed marked reduction of the motor action potential amplitudes and conduction velocities with pathologic spontaneous activity. In conclusion, we found no peripheral nerve dysfunction/degeneration as signs of a CIP-like phenotype after MCAo.


2020 ◽  
Author(s):  
Petra Huehnchen ◽  
Klaus Viktor Toyka ◽  
Karen Gertz ◽  
Matthias Endres ◽  
Wolfgang Boehmerle

Abstract Objective: Critical illness polyneuropathy (CIP) is a common complication of severe systemic illness treated in intensive care medicine. Ischemic stroke leads to an acute critical injury of the brain with hemiparesis, immunosuppression and subsequent infections, all of which require extended medical treatment. Stroke-induced sarcopenia further contributes to poor rehabilitation and is characterized by muscle wasting and denervation in the paralytic, but also the unaffected limbs. Therefore, we asked whether stroke leads to an additional CIP-like neurodegeneration.Results: Focal brain ischemia was induced in adult mice by 60-minute middle cerebral artery occlusion (MCAo) following reperfusion and led to functional deficits and marked hemispheric brain atrophy. Nerve conduction function and muscle potentials were measured in the ipsilateral sciatic nerve and gastrocnemius and quadriceps muscle with electroneurography/-myography on days 10, 22, 44 after stroke. An additional crush-injury to the sciatic nerve was included in two sham mice as positive control (sham+). We found no differences in nerve conduction function nor spontaneous electromyographic activity between MCAo and sham animals. Sham+ mice developed marked reduction of the motor action potential amplitudes and conduction velocities with pathologic spontaneous activity. In conclusion, we found no peripheral nerve dysfunction/ degeneration as signs of a CIP-like phenotype after MCAo.


2020 ◽  
Author(s):  
Petra Huehnchen ◽  
Klaus Viktor Toyka ◽  
Karen Gertz ◽  
Matthias Endres ◽  
Wolfgang Boehmerle

Abstract Objective: Critical illness polyneuropathy (CIP) is a common complication of severe systemic illness treated in intensive care medicine. Ischemic stroke leads to an acute critical injury of the brain with hemiparesis, immunosuppression and subsequent infections, all of which require extended medical treatment. Stroke-induced sarcopenia further contributes to poor rehabilitation and is characterized by muscle wasting and denervation in the paralytic, but also the unaffected limbs. Therefore, we asked whether stroke leads to an additional CIP-like neurodegeneration. Results: Focal brain ischemia was induced in adult mice by 60-minute middle cerebral artery occlusion (MCAo) following reperfusion and led to functional deficits and marked hemispheric brain atrophy. Nerve conduction function and muscle potentials were measured in the ipsilateral sciatic nerve and gastrocnemius and quadriceps muscle with electroneurography/-myography on days 10, 22, 44 after stroke. An additional crush-injury to the sciatic nerve was included in two sham mice as positive control (sham+). We found no differences in nerve conduction function nor spontaneous electromyographic activity between MCAo and sham animals. Sham+ mice developed marked reduction of the motor action potential amplitudes and conduction velocities with pathologic spontaneous activity. In conclusion, we found no peripheral nerve dysfunction/ degeneration as signs of a CIP-like phenotype after MCAo.


2020 ◽  
Author(s):  
Petra Huehnchen ◽  
Klaus Viktor Toyka ◽  
Karen Gertz ◽  
Matthias Endres ◽  
Wolfgang Boehmerle

Abstract Objective: Critical illness polyneuropathy (CIP) is a common complication of severe systemic illness treated in intensive care medicine. Ischemic stroke leads to an acute critical injury of the brain with hemiparesis, immunosuppression and subsequent infections, all of which require extended medical treatment. Stroke-induced sarcopenia further contributes to poor rehabilitation and is characterized by muscle wasting and denervation in the paralytic, but also the unaffected limbs. Therefore, we asked whether stroke leads to an additional CIP-like neurodegeneration. Results: Focal brain ischemia was induced in adult mice by 60-minute middle cerebral artery occlusion (MCAo) following reperfusion and led to functional deficits and marked hemispheric brain atrophy. Nerve conduction function and muscle potentials were measured in the ipsilateral sciatic nerve and gastrocnemius and quadriceps muscle with electroneurography/-myography on days 10, 22, 44 after stroke. An additional crush-injury to the sciatic nerve was included in two sham mice as positive control (sham+). We found no differences in nerve conduction function nor spontaneous electromyographic activity between MCAo and sham animals. Sham+ mice developed marked reduction of the motor action potential amplitudes and conduction velocities with pathologic spontaneous activity. In conclusion, we found no peripheral nerve dysfunction/ degeneration as signs of a CIP-like phenotype after MCAo.


2020 ◽  
Author(s):  
Petra Huehnchen ◽  
Klaus Viktor Toyka ◽  
Karen Gertz ◽  
Matthias Endres ◽  
Wolfgang Boehmerle

Abstract Objective Critical illness polyneuropathy (CIP) is a common complication of severe systemic illness treated in intensive care medicine. Ischemic stroke leads to an acute critical injury of the brain with hemiparesis, immunosuppression and subsequent infections, all of which require extended medical treatment. Stroke-induced sarcopenia further contributes to poor rehabilitation and is characterized by muscle wasting and denervation in the paralytic, but also the unaffected limbs. Therefore, we asked whether stroke leads to an additional CIP-like neurodegeneration. Results Focal brain ischemia was induced in adult mice by 60-minute middle cerebral artery occlusion (MCAo) following reperfusion and led to functional deficits and marked hemispheric brain atrophy. Nerve conduction function and muscle potentials were measured in the ipsilateral sciatic nerve and gastrocnemius and quadriceps muscle with electroneurography/-myography on days 10, 22, 44 after stroke. An additional crush-injury to the sciatic nerve was included in two sham mice as positive control (sham+). We found no differences in nerve conduction function nor spontaneous electromyographic activity between MCAo and sham animals. Sham + mice developed marked reduction of the motor action potential amplitudes and conduction velocities with pathologic spontaneous activity.


2019 ◽  
Vol 148 ◽  
pp. 347-357 ◽  
Author(s):  
Tianyi Wang ◽  
Bo Li ◽  
Zhijie Wang ◽  
Xin Wang ◽  
Ziwei Xia ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document