small hepatocytes
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2021 ◽  
Author(s):  
Naoki Tanimizu ◽  
Norihisa Ichinohe ◽  
Yasushi Sasaki ◽  
Tohru Itoh ◽  
Ryo Sudo ◽  
...  

Abstract Number of liver organoids have been reported, though it is not clearly shown whether the functional connection between hepatocytes and cholangiocytes is recapitulated in those organoids. Here, we report generation of a hepatobiliary tubular organoid (HBTO) using mouse hepatocyte progenitors called small hepatocytes (SHs) and cholangiocytes. SHs differentiate and form the bile canalicular network in HBTOs and secret metabolites into the canaliculi, which are then transported into the biliary structure. Hepatocytes in the organoid acquire and maintain metabolic functions including albumin secretion and cytochrome P450 activities, over the long term. We provide the step-by-step protocol for induction of HBTO including isolation of cholangiocytes and SHs and co-culture of these two types of cell to generate functional connections between hepatocytes and cholangiocytes.


2019 ◽  
Vol 98 (2) ◽  
pp. 717-721
Author(s):  
H. Fukasawa ◽  
H. Hashimoto ◽  
K. Hiramoto ◽  
M. Sugiyama ◽  
K. Taniguchi ◽  
...  

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Masayuki Ishii ◽  
Junichi Kino ◽  
Norihisa Ichinohe ◽  
Naoki Tanimizu ◽  
Takafumi Ninomiya ◽  
...  

2012 ◽  
Vol 227 (6) ◽  
pp. 2363-2370 ◽  
Author(s):  
Hidekazu Ooe ◽  
Qijie Chen ◽  
Junko Kon ◽  
Kazunori Sasaki ◽  
Hiroyuki Miyoshi ◽  
...  
Keyword(s):  

2009 ◽  
Vol 41 (9) ◽  
pp. 3887-3892 ◽  
Author(s):  
H. Zhang ◽  
Z. Liu ◽  
R. Li ◽  
D. Wang ◽  
W. Liu ◽  
...  

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
D Knobeloch ◽  
P Kupczyk ◽  
A Lehmann ◽  
M Glanemann ◽  
N Liu ◽  
...  

Author(s):  
D. Knobeloch ◽  
P. Kupczyk ◽  
A. Lehmann ◽  
N. Liu ◽  
M. Glanemann ◽  
...  
Keyword(s):  

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