nisin yield
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2020 ◽  
Vol 86 (9) ◽  
Author(s):  
Hao Wu ◽  
Ershu Xue ◽  
Ning Zhi ◽  
Qianqian Song ◽  
Kairen Tian ◽  
...  

ABSTRACT Lactococcus lactis encounters various environmental challenges, especially acid stress, during its growth. The cell wall can maintain the integrity and shape of the cell under environmental stress, and d-amino acids play an important role in cell wall synthesis. Here, by analyzing the effects of 19 different d-amino acids on the physiology of L. lactis F44, we found that exogenously supplied d-methionine and d-phenylalanine increased the nisin yield by 93.22% and 101.29%, respectively, as well as significantly increasing the acid resistance of L. lactis F44. The composition of the cell wall in L. lactis F44 with exogenously supplied d-Met or d-Phe was further investigated via a vancomycin fluorescence experiment and a liquid chromatography-mass spectrometry assay, which demonstrated that d-Met could be incorporated into the fifth position of peptidoglycan (PG) muropeptides and d-Phe could be added to the fourth and fifth positions. Moreover, overexpression of the PG synthesis gene murF further enhanced the levels of d-Met and d-Phe involved in PG and increased the survival rate under acid stress and the nisin yield of the strain. This study reveals that the exogenous supply of d-Met or d-Phe can change the composition of the cell wall and influence acid tolerance as well as nisin yield in L. lactis. IMPORTANCE As d-amino acids play an important role in cell wall synthesis, we analyzed the effects of 19 different d-amino acids on L. lactis F44, demonstrating that d-Met and d-Phe can participate in peptidoglycan (PG) synthesis and improve the acid resistance and nisin yield of this strain. murF overexpression further increased the levels of d-Met and d-Phe incorporated into PG and contributed to the acid resistance of the strain. These findings suggest that d-Met and d-Phe can be incorporated into PG to improve the acid resistance and nisin yield of L. lactis, and this study provides new ideas for the enhancement of nisin production.


2020 ◽  
Vol 51 (3) ◽  
pp. 1247-1257
Author(s):  
Zeynep Girgin Ersoy ◽  
Ceyhun Kayıhan ◽  
Sedef Tunca

2018 ◽  
Vol 40 (6) ◽  
pp. 941-948 ◽  
Author(s):  
Sen Miao ◽  
Hao Wu ◽  
Yue Zhao ◽  
Qinggele Caiyin ◽  
Yanni Li ◽  
...  

2018 ◽  
Vol 84 (6) ◽  
Author(s):  
Hao Wu ◽  
Jingui Liu ◽  
Sen Miao ◽  
Yue Zhao ◽  
Hongji Zhu ◽  
...  

ABSTRACTTo overcome the adverse impacts of environmental stresses during growth, different adaptive regulation mechanisms can be activated inLactococcus lactis. In this study, the transcription levels of eight transcriptional regulators ofL. lactissubsp.lactisF44 under acid stress were analyzed using quantitative reverse transcription-PCR. Eight gene-overexpressing strains were then constructed to examine their influences on acid-resistant capability. OverexpressingythA, a PspC family transcriptional regulator, increased the survival rate by 3.2-fold compared to the control at the lethal pH 3.0 acid shock. Moreover, the nisin yield was increased by 45.50%. TheythA-overexpressing strain FythA appeared to have higher intracellular pH stability and nisin-resistant ability. Subsequently, transcriptome analysis revealed that the vast majority of genes associated with amino acid biosynthesis, including arginine, serine, phenylalanine, and tyrosine, were predominantly upregulated in FythA. Arginine biosynthesis (argGandargH), arginine deiminase pathway, and polar amino acid transport (ysfEandysfF) were proposed to be the main regulation mechanisms of YthA. Furthermore, the transcription of genes associated with pyrimidine and exopolysaccharide biosynthesis were upregulated. The transcriptional levels ofnisIPRKFEGgenes were substantially higher in FythA, which directly contributed to the yield and resistance of nisin. Three potential DNA-binding sequences were predicted by computer analysis using the upstream regions of genes with prominent changes. This study showed that YthA could increase acid resistance and nisin yield and revealed a putative regulation mechanism of YthA.IMPORTANCENisin, produced byLactococcus lactissubsp.lactis, is widely used as a safe food preservative. Acid stress becomes the primary restrictive factor of cell growth and nisin yield. In this research, we found that the transcriptional regulator YthA was conducive to enhancing the acid resistance ofL. lactisF44. OverexpressingythAcould significantly improve the survival rate and nisin yield. The stability of intracellular pH and nisin resistance were also increased. Transcriptome analysis showed that nisin immunity and the biosynthesis of some amino acids, pyrimidine, and exopolysaccharides were enhanced in the engineered strain. This study elucidates the regulation mechanism of YthA and provides a novel strategy for constructing robust industrialL. lactisstrains.


2017 ◽  
Vol 101 (16) ◽  
pp. 6483-6493 ◽  
Author(s):  
Jiakun Qi ◽  
Qinggele Caiyin ◽  
Hao Wu ◽  
Kairen Tian ◽  
Binbin Wang ◽  
...  

2017 ◽  
Vol 101 (15) ◽  
pp. 6137-6153 ◽  
Author(s):  
Panlong Hao ◽  
Dongmei Liang ◽  
Lijie Cao ◽  
Bin Qiao ◽  
Hao Wu ◽  
...  

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jian Zhang ◽  
Qinggele Caiyin ◽  
Wenjing Feng ◽  
Xiuli Zhao ◽  
Bin Qiao ◽  
...  

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