scholarly journals Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jian Zhang ◽  
Qinggele Caiyin ◽  
Wenjing Feng ◽  
Xiuli Zhao ◽  
Bin Qiao ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Acid Tolerant ◽  
Nisin Yield

Promoting acid resistance and nisin yield of Lactococcus lactis F44 by genetically increasing D-Asp amidation level inside cell wall

2017 ◽  
Vol 101 (15) ◽  
pp. 6137-6153 ◽  
Author(s):  
Panlong Hao ◽  
Dongmei Liang ◽  
Lijie Cao ◽  
Bin Qiao ◽  
Hao Wu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Lactococcus Lactis ◽  
Acid Resistance ◽  
Nisin Yield

scholarly journals Higher nisin yield is reached with glutathione and pyruvate compared with heme in Lactococcus lactis N8

2020 ◽  
Vol 51 (3) ◽  
pp. 1247-1257
Author(s):  
Zeynep Girgin Ersoy ◽  
Ceyhun Kayıhan ◽  
Sedef Tunca
Keyword(s):  
Lactococcus Lactis ◽  
Nisin Yield

scholarly journals D-Methionine and D-Phenylalanine Improve Lactococcus lactis F44 Acid Resistance and Nisin Yield by Governing Cell Wall Remodeling

2020 ◽  
Vol 86 (9) ◽  
Author(s):  
Hao Wu ◽  
Ershu Xue ◽  
Ning Zhi ◽  
Qianqian Song ◽  
Kairen Tian ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cell Wall ◽  
Lactococcus Lactis ◽  
Acid Stress ◽  
Acid Tolerance ◽  
Acid Resistance ◽  
Cell Wall Synthesis ◽  
Nisin Production ◽  
Content Type ◽  
Nisin Yield

ABSTRACT Lactococcus lactis encounters various environmental challenges, especially acid stress, during its growth. The cell wall can maintain the integrity and shape of the cell under environmental stress, and d-amino acids play an important role in cell wall synthesis. Here, by analyzing the effects of 19 different d-amino acids on the physiology of L. lactis F44, we found that exogenously supplied d-methionine and d-phenylalanine increased the nisin yield by 93.22% and 101.29%, respectively, as well as significantly increasing the acid resistance of L. lactis F44. The composition of the cell wall in L. lactis F44 with exogenously supplied d-Met or d-Phe was further investigated via a vancomycin fluorescence experiment and a liquid chromatography-mass spectrometry assay, which demonstrated that d-Met could be incorporated into the fifth position of peptidoglycan (PG) muropeptides and d-Phe could be added to the fourth and fifth positions. Moreover, overexpression of the PG synthesis gene murF further enhanced the levels of d-Met and d-Phe involved in PG and increased the survival rate under acid stress and the nisin yield of the strain. This study reveals that the exogenous supply of d-Met or d-Phe can change the composition of the cell wall and influence acid tolerance as well as nisin yield in L. lactis. IMPORTANCE As d-amino acids play an important role in cell wall synthesis, we analyzed the effects of 19 different d-amino acids on L. lactis F44, demonstrating that d-Met and d-Phe can participate in peptidoglycan (PG) synthesis and improve the acid resistance and nisin yield of this strain. murF overexpression further increased the levels of d-Met and d-Phe incorporated into PG and contributed to the acid resistance of the strain. These findings suggest that d-Met and d-Phe can be incorporated into PG to improve the acid resistance and nisin yield of L. lactis, and this study provides new ideas for the enhancement of nisin production.

The acid tolerant and cold-active β-galactosidase from Lactococcus lactis strain is an attractive biocatalyst for lactose hydrolysis

2012 ◽  
Vol 103 (4) ◽  
pp. 701-712 ◽  
Author(s):  
Violette Vincent ◽  
Nushin Aghajari ◽  
Noémie Pollet ◽  
Anaïs Boisson ◽  
Samira Boudebbouze ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Lactose Hydrolysis ◽  
Cold Active ◽  
Acid Tolerant ◽  
Lactis Strain

Enhancing nisin yield by engineering a small noncodding RNA anti41 and inhibiting the expression of glnR in Lactococcus lactis F44

2018 ◽  
Vol 40 (6) ◽  
pp. 941-948 ◽  
Author(s):  
Sen Miao ◽  
Hao Wu ◽  
Yue Zhao ◽  
Qinggele Caiyin ◽  
Yanni Li ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Nisin Yield

The novel sRNA s015 improves nisin yield by increasing acid tolerance of Lactococcus lactis F44

2017 ◽  
Vol 101 (16) ◽  
pp. 6483-6493 ◽  
Author(s):  
Jiakun Qi ◽  
Qinggele Caiyin ◽  
Hao Wu ◽  
Kairen Tian ◽  
Binbin Wang ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Acid Tolerance ◽  
The Novel ◽  
Nisin Yield

Exportation de protéines hétérologues chez Lactococcus lactis : développement d'outils moléculaires et applications

2000 ◽  
Vol 20 (1) ◽  
pp. 135-142
Author(s):  
Philippe Langella ◽  
Yves Le Loir ◽  
Jean-Christophe Piard ◽  
Isabelle Pocquet ◽  
Alexandra Gruss
Keyword(s):  
Lactococcus Lactis

Production et sécrétion de L7/L12, un antigène de Brucella abortus, chez Lactococcus lactis : vers de nouveaux vaccins oraux anti-brucellose ?

2002 ◽  
Vol 22 (1-2) ◽  
pp. 199-208 ◽  
Author(s):  
Lucian A Ribeiro ◽  
Vasco Azevedo ◽  
Yves Le Loir ◽  
Daniela Pontes ◽  
Sergio C Oliveira ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Brucella Abortus
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