Effects of Compostable Packaging and Perforation Rates on Cucumber Quality during Extended Shelf Life and Simulated Farm-to-Fork Supply-Chain Conditions

Cucumbers are highly perishable and suffer from moisture loss, shriveling, yellowing, peel damage, and decay. Plastic packaging helps to preserve cucumber quality, but harms the environment. We examined the use of compostable modified atmosphere packaging (MAP) with different perforation rates as a possible replacement for conventional plastic packaging materials. The results indicate that all of the tested types of packaging reduced cucumber weight loss and shriveling. However, compostable MAP with micro-perforations that created a modified atmosphere of between 16–18% O2 and 3–5% CO2 most effectively preserved cucumber quality, as demonstrated by reduced peel pitting, the reduced appearance of warts and the inhibition of yellowing and decay development. Overall, micro-perforated compostable packaging extended the storage life of cucumbers under both extended shelf conditions and simulated farm-to-fork supply-chain conditions and thus may serve as a replacement for the plastic packaging currently used to preserve the postharvest quality of cucumbers.

Role of Leaf Litter in Above-Ground Wood Decay

The effects of leaf litter on moisture content and fungal decay development in above-ground wood specimens were assessed. Untreated southern pine specimens were exposed with or without leaf litter contact. Two types of leaf litter were evaluated; aged (decomposed) and young (early stages of decomposition). The moisture content of specimens was monitored, and specimens were periodically removed for visual evaluation of decay development. In addition, amplicon-based sequencing analysis of specimens and associated leaf litter was conducted at two time points. Contact with either type of leaf litter resulted in consistently higher moisture contents than those not in contact with leaf litter. Visually, evident decay developed most rapidly in specimens in contact with the aged leaf litter. Analysis of amplicon-based sequencing revealed that leaf litter contributes a significant amount of the available wood decay fungal community with similar communities found in the litter exposed wood and litter itself, but dissimilar community profiles from unexposed wood. Dominant species and guild composition shifted over time, beginning initially with more leaf saprophytes (ascomycetes) and over time shifting to more wood rotting fungi (basidiomycetes). These results highlight the importance of the contributions of leaf litter to fungal colonization and subsequent decay hazard for above-ground wood.

The effect of ambient pH modulation on ochratoxin A accumulation by Aspergillus carbonarius

Aspergillus carbonarius, the main cause of severe post-harvest decay of vine fruit, is considered the major source of ochratoxin A (OTA) contamination of grapes and derived products. The factors inducing OTA accumulation by A. carbonarius and its contribution to pathogenicity remain unclear. Present findings indicate that the production of organic acids, such as D-gluconic acid (GLA) and citric acid, by A. carbonarius in the growth medium or in the decayed fruit tissue was directly related to ambient pH reduction. Under these conditions, induced transcript expression of genes involved in OTA biosynthesis occurred concurrently with mycotoxin accumulation. The high accumulation of OTA during acidification process raised the question of its importance in host-pathogen interactions during the fungal colonisation. Treatment of colonised grapes with sodium bicarbonate reduced accumulation of organic acid and OTA with a concomitant reduction in decay development, suggesting that tissue acidification is a significant factor in A. carbonarius pathogenicity. The present findings suggest that ambient pH is a regulatory signal for induction of mycotoxin production by A. carbonarius under the dynamic nutritional growth conditions occurring in culture. Yet the molecular mechanisms of OTA biosynthesis induction during colonisation of the acidic host environment are still unclear and should be further investigated.

The Management of Tree Root Systems in Urban and Suburban Settings II: A Review of Strategies to Mitigate Human Impacts

Root systems of nearly all trees in the built environment are subject to impacts of human activities that can affect tree health and reduce longevity. These influences are present from early stages of nursery development and throughout the life of the tree. Reduced root systems from root loss or constriction can reduce stability and increase stress. Natural infection of urban tree roots after severing has not been shown to lead to extensive decay development. Roots often conflict with infrastructure in urban areas because of proximity. Strategies to provide root space under pavements and to reduce pavement heaving have been developed, but strategies for prevention of foundation and sewer pipe damage are limited to increasing separation or improved construction.