Volatile Organic Compound Profiles Associated with Microbial Development in Feedlot Pellets Inoculated with Bacillus amyloliquefaciens H57 Probiotic

Mould and bacterial contamination releases microbial volatile organic compounds (mVOCs), causing changes in the odour profile of a feed. Bacillus amyloliquefaciens strain H57 (H57) has the potential ability to inhibit microbial growth in animal feeds. This study tested the hypothesis that H57 influences the odour profile of stored feedlot pellets by impeding the production of mVOCs. The emission of volatile organic compounds (VOCs) of un-inoculated pellets and those inoculated with H57, stored either at ambient temperature (mean 22 °C) or at 5 °C, was monitored at 0, 1, 2, and 3 months by gas chromatography–mass spectrometry. Forty VOCs were identified in all the pellet samples analysed, 24 of which were potentially of microbial and 16 of non-microbial origin. A score plot of the principal component analysis (PCA) showed that the VOC profiles of the pellets stored at ambient temperature changed more rapidly over the 3 months than those stored at 5 °C, and that change was greater in the un-inoculated pellets when compared to the inoculated ones. The bi-plot and correlation loading plots of the PCA indicated that the separation of the un-inoculated pellets from the other treatments over the 3 months was primarily due to nine mVOCs. These mVOCs have been previously identified in grains spoiled by fungi, and could be considered potential markers of the types of fungi that H57 can protect pellets against. These data indicate the ability of H57 to maintain the odour profile and freshness of concentrated feed pellets. This protective influence can be detected as early as 3 months into ambient temperature storage.

Feed Preference Response of Weaner Bull Calves to Bacillus amyloliquefaciens H57 Probiotic and Associated Volatile Organic Compounds in High Concentrate Feed Pellets

This study tested the hypothesis that Bacillus amyloliquefaciens strain H57 (H57) improves preference by reducing the development of microbial volatile organic compounds (mVOCs) in feed pellets. Sixteen bull calves were, for 4 weeks, provided equal access to a panel of 8 automated feed bunks in a single paddock with some hay. Each bunk contained pellets with (H57) or without (Control) the H57, each aged for 4 months at either ambient or chiller temperature. Each treatment was changed to a new bunk pair position weekly. Relative preference was determined according to weight of pellets remaining per hour per treatment bunk pair per 24 h. Pellets were analysed for volatile organic compounds (VOCs) and the concentrations tested for correlation with relative preference. Calves showed the lowest preference (p < 0.0001) for the Control/Ambient treatment whereas preference for all other treatments (H57/Ambient; H57/Chiller; Control/Chiller) was similar. The Control/Ambient treatment odour profile grouped differently to the other 3 treatments which grouped similarly to each other. Up to 16 mVOCs were determined to have potential as pre-ingestive signals for the extent of microbial spoilage. Further studies are required to find which combination of these mVOCs, when added to pellets, results in feed aversion.

Intelligent Classification of Palm Oil Tree Pollination Using E-Nose

The pollination period is one of the crucial steps needed to ensure crop yield increases, especially in palm oil palm plantations. Most of the research has difficulty determining the pollination period of palm oil. Many problems contribute to this problem, such as difficut to reach and depedency of the polination insect as the insect activity is influenced by the surrounding enviroment.E-Nose can help determine the period by classifiy odour pattern of the male and female palm oil flower. The pattern of each of the flowers were classified using cased – based reasoning artificial intelligent technique. This paper shows the research of the palm oil pollination flower odour profile pattern using case-based reasoning (CBR) classifier.

Species Specificity of the Putative Male Antennal Aphrodisiac Pheromone inLeptopilina heterotoma,Leptopilina boulardi, andLeptopilina victoriae

Male antennal aphrodisiac pheromones have been suggested to elicit female receptiveness in several parasitic Hymenoptera, includingLeptopilina boulardi. None of the proposed pheromones, however, has been fully identified to date. It is also unknown whether these antennal pheromones are species specific, because the species specificity of mate recognition and courtship elicitation inLeptopilinaprevented such experiments. In this study we present an experimental design that allows the investigation of the species specificity of the putative male aphrodisiac pheromone ofL. heterotoma, L. boulardi,andL. victoriae. This is achieved by chemical manipulation of the odour profile of heterospecific females, so that males perceive them as conspecifics and show antennal courtship behaviour. Males courted the manipulated heterospecific females and antennal contact between the male and the female was observed. However, males elicited receptiveness only in conspecific females, never in the manipulated heterospecific females. Chemical analysis showed the presence of species specific unsaturated hydrocarbons on the antennae of males. Only trace amounts of these hydrocarbons are found on the antennae of females. Our results are an important step towards the understanding and identification of antennal pheromones of parasitic wasps.

Tomato Quality during Short-Term Storage Assessed by Colour and Electronic Nose

An assay based on an electronic olfactory system was set to evaluate tomato fruits by sensing the aromatic volatiles during postharvest storage of 21 days at C in darkness. Olfactory system measurements were coupled with colour values. Odour profile and senescence parameters were carried out at 7-day intervals. Discriminant function analysis applied to electronic nose data showed three components, accounting for 99.2% of the total variance. In the present assay, separation among groups according to storage time (0, 7, and 14 days) was observed for wildtype. Overexpressed (Money Maker) lines/plants of tomato showed difference between odour profile for day 0 and day 21, even tough a no clear discrimination between 7 and 14 days was observed. Fruit lost weight almost linearly with shelf life () presenting an averaged loss of 21% () for over-expressed (Money Maker) lines/plants, 13% () for silenced (Money Maker), and 14% () for wild type during 21 days of storage. Colour values , , and data showed that colour properties changed during storage for all the lines considered. Correlations between odour profiles and colour parameter were obtained showing that the electronic nose is a useful technique for monitoring short-term storage of tomato.