Complements C3 and C4 in serum and stimulatedsaliva of patients suffer oral erosive lichen planus

Introduction: The objective of this study was to compare the level of complements C3 and C4 in serum, and stimulated saliva between oral lichen planus (OLP) and healthy individuals. Methods: A case-control study was performed on 31 healthy and 31 who suffer the erosive type of OLP. Serum and saliva level of C3 and C4 were measured by immunoturbidimetry method. Results: C3 and C4 were expressed at a lower level in serum and saliva of OLP patients compared to control groups. Serum C3 and C4 levels did not correlate with their saliva levels. The receiver operating characteristic analysis showed significantly diagnostic abilities for serum and saliva C3 and C4 to discrimination of OLP patients from controls (cutoff [mg/dl] for C3 were 83 in serum and 3.45 in saliva and for C4 were 9.5 in serum and 0.9 in saliva). Conclusion: Serum and salivary levels of total C3 and C4 were lower in patients with OLP than in healthy controls. Therefore, they may able to discriminate OLP from healthy.

Does Pepsin Saliva Concentration (Peptest™) Predict the Therapeutic Response of Laryngopharyngeal Reflux Patients?

Objective: To study the profile and the therapeutic response of patients with laryngopharyngeal reflux (LPR) at the hypopharyngeal-esophageal multichannel intraluminal impedance-pH monitoring (HEMII-pH) according to the initial pepsin saliva concentration. Methods: From January 2018 to January 2020, patients with positive LPR diagnosis at the HEMII-pH were consecutively recruited from 3 European Hospitals. Saliva pepsin concentration (Peptest™) was measured during the HEMII-pH testing period and patients were classified into 2 groups: negative versus positive Peptest. The clinical outcomes, that is, gastrointestinal and HEMII-pH findings, reflux symptom score-12 (RSS-12), and 3-month therapeutic response, were compared between groups. Results: A total of 124 patients completed the study. Among them, 30 patients had negative Peptest. Pharyngeal reflux events occurred outside 1-hour post-meal time in 74.0%, after the meals in 20.5% and nighttime in 5.5%. The pepsin saliva level was not significantly associated with the reflux events preceding the sample collection. Patients with positive Peptest had better improvement of RSS-12 digestive and respiratory subscores and oral, pharyngeal, and laryngeal findings compared with patients with negative Peptest. Conclusion: Patients with high saliva pepsin concentration had no stronger gastrointestinal, HEMII-pH, or clinical outcomes compared with those with low or undetectable saliva pepsin concentration.

Serum and Saliva Myelin Basic Protein as Multiple Sclerosis Biomarker

Objective: Multiple sclerosis (MS) is considered as motor and sensory function loss, which results from demyelination and following axonal lesion. As Myelin basic protein (MBP) is one of the key elements of the myelin cover, we examined the level of MBP in serum, stimulated and unstimulated saliva as a suitable biomarker for detecting of MS. Methods: A case–control study was performed in 29 healthy women and 32 definitive relapsing-remitting MS patients who were hospitalized in Imam Reza hospital, Tehran, Iran. MBP level was assayed in serum and stimulated and unstimulated whole saliva. Results: MBP was expressed at a lower level in serum and stimulated saliva of MS patients compared to control groups. The serum MBP level had a considerable correlation with its stimulated saliva level. The receiver operating characteristic analysis showed significantly diagnostic abilities for MBP to discrimination MS patients with serum and stimulated saliva from controls. Conclusions: Serum and saliva level of MBP is lower in MS and it may be considered as a potential biomarker in Multiple sclerosis.

Hybrid hydrogel films with graphene oxide for continuous saliva-level monitoring

Hybrid hydrogel films with graphene oxide were designed and coated on a quartz crystal microbalance (QCM) to continuously monitor the saliva glucose, resulting in a greatly-improved limit of detection.

The validity of carbohydrate antigen 19-9 in serum and saliva as a new type for diagnosis in Iraqi kids having cystic fibrosis

Cystic fibrosis is a life-limiting, recessive disease; it occurs due to mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Carbohydrate antigen 19-9 (CA19.9) is a tumour-associated, primarily occur in patients having biliary tract and pancreatic cancers but are also observed in patients having other malignancies. Conditions related to benign like cholestasis, cirrhosis, cholangitis, plus pancreatitis also result in CA 19-9 elevations. Raised levels of serum CA 19-9 seems in CF patient to be related to disease pulmonary exacerbation also in the lung the amount of sputum. Investigate whether serum and saliva (CA19.9) level may contribute to cystic fibrosis diagnosis establishment in patients. This "case-control study" consists of 80 individuals (30 patients and 50 healthy controls). Their age was range between one month and 18 years. Blood and saliva samples were taken from patients. Saliva level in patients was 1.775±1.030U/ml (P<0.001) as compared to control group (0.956±0.682 U/ml). A serum level of CA19.9 was found to be increased (P<0.001) by 0.818±0.601 U/ml in CF patients as compared to healthy (0.334±0.101 U/ml) control. From this study, we can conclude that the salivary CA19.9 level can be diagnosed as a marker for cystic fibrosis.

HUBUNGAN KADAR UREA SALIVA TERHADAP DERAJAT KEASAMAN (pH) SALIVA PADA ANAK USIA 12-15 TAHUN

Introduction: One of the dental disease in the oral cavity that often experience by society are caries. Caries is one of the hard tooth tissue disease that can touch enamel, dentin, and sementum. One of the factor that affect caries process is saliva. Urea is one of the organic composition that compose saliva. Urea in salive plays as buffer capacity and the pH saliva adjustment.Methods: This study is an analytic observational with cross sectional approach, this study are conducted in Daarul 'Ilmi Islamic boarding school with 19 subject of 12-15 years old child. The data are obtained from the examination of urea saliva level and pH saliva in child. The level of urea saliva are measured using spectrophotometer tools and the pH saliva are measured using pH meter. The Shapiro-wilkare used to deterime the data normality, continue with Leuvenetest to determinde the data homogeneity data. Data are analyzed using Pearson test to determine the relation of urea level in saliva against the pH saliva.Results: According the data analysis, the data are in normal distribution and homogeneous. The research result are obtain average of urea level in urea sample is 13,45 mg/dl, and the average of pH saliva sampelis 7,15. The result of Pearsonstatistic testare obtain p value of = 0,000, with the positive correlation level of 0,902.Conclusion: The conclusion of this study are note that there are relation between the urea level in saliva against pH salive. This are proven from the Pearson test result with significance value of 0.000 (p< 0.05).

Statherin saliva levels in patients with dental caries

Aim. To examine the influence of statherin on the rate of dental diseases and to determine the concentration of salivary staterin in patients with dental caries. Methods. The study recruited 134 persons, including 54 patients with dental caries and its complications (experimental group) and 80 healthy volunteers (control group). Pateints’ mean age ranged from 18 to 42 years. A comprehensive dental examination of all subjects was carried out, hygiene indexes were defined, complete dental history was gathered. Whole unstimulated saliva was examined. Statherin saliva level was determined using an enzyme-linked immunoassay test. To study the relationship between statherin saliva level and studied dental clinical features (amount of teeth with caries, sealed and extracted teeth, age, hygiene indices, eating and smoking habits, xerostomia), Spearman nonparametric rank correlation test was used. Results. Statherin saliva level was higher in patients with dental caries and its complications compared to healthy controls. Mean saliva statherin concentration was Me=6.1 [2.5, 18.4] mmol/l in patients with caries compared to Me=3.5 [2.9, 7.3] mmol/l in control group. There was no statistically significant differences of saliva staterin concentrations between men and women in both groups. Oral hygiene (as defined by simplified oral hygiene index), food consumption (right after a meal or 4 hours after a meal), bad habits (smoking) had no effect on statherin concentrations in saliva. Conclusions. Statherin saliva levels are indicative of the diseases of the oral cavity.