Inhibitory effect of tetramethylpyrazine combined with propranolol on murine hemangioma endothelial cells

Purpose: To study the inhibitory effect of different doses of tetramethylpyrazine (TMP) combined with the beta-blocker, propranolol (Pro) on hemangioma endothelial (EOMA) cells. Methods: EOMA cells were cultured in vitro with varying doses of TMP and Pro (5, 10, 20 and 40 uM). The effect of treatments on cell proliferation was assessed by MTT assay, while cell apoptosis was assayed by flow cytometry. The expressions of Bcl-2, Bax, p-mTOR), total-mammalian target of rapamycin (t-mTOR, p-p70S6) and total-p70 ribosomal protein S6 (t-p70S6) proteins were determined using Western blot. Results: MTT data showed that when used alone, TMP had no significant inhibitory effect on EOMA cells (p > 0.05). However, when TMP was combined with propranolol, there was significant inhibition of EOMA cells, and that the inhibition is dependent on TMP dose. Flow cytometry results showed that the combination of TMP and Pro induced EOMA cell apoptosis dose-dependently (p < 0.05). Moreover, TMP dose-dependently inhibited the phosphorylation of mTOR and p70S6 in EOMA cells, and enhanced Bax expression, but downregulated Bcl-2 (p < 0.05). Conclusion: These results suggest that TMP enhances the inhibitory influence of Pro p-mTOR and pp-70S6 in EOMA cells in a dose-dependent manner. Thus, TMP may enhance Pro-induced inhibition of the growth of endothelial cells, and promote apoptosis through suppression of activation of PI3K/AKT signal route. These findings provide a theoretical basis for the clinical application of TMP/Pro combination for the treatment of hemangioma.

Karakteristik CCR Dan Efesiensi Kanal Pada Penerapan Trafik Signalling Dengan Metode LSHB

This paper reports the investigation of the characteristic of call completion rate (CCR) and channelefficiency for the application of the Loadsharing Bit (LSHB) methode in the signalling. The model of LSHB isdevided to be six LSHB by varying two Signal Route Trunk Equipment (SRTE) and sixteen Signal Link Selection(SLS). The measurement was carried out in Palembang MSC related to its BSC and to Medan MSC andPakanbaru MSC, where the network was on. The results showed that by using the LSHB method, the CCR couldbe improved, and the channel efficiency was not so be influented yet, becaused the data was still low.

Frequency Response Characteristics of FM Mini-Loop Auditory Trainers

Personally sized inductance loops (mini-loops) now are available for use with an FM classroom amplification system and the student's personal ear-level hearing aid. Frequency response characteristics of five commercially-available hearing aids were assessed 1) using hearing aid test equipment available in most audiology clinics, with the hearing aid on microphone setting and 2) in a public school classroom, with the hearing aid on telecoil setting and operating with an FM mini-loop system. Clinic and classroom characteristics of the conventional FM receiver-insert earphone auditory trainers also were assessed. Results showed that the personal hearing aids' classroom performance could not be predicted from their clinic performance. Results also showed that gain provided by the environmental microphone circuit of the FM receiver-insert earphone units was uniformly higher than that provided by the teacher-microphone signal route.