scholarly journals Spectrophotometric Estimation of Abacavir Sulphate in Pharmaceutical Formulations

2008 ◽  
Vol 5 (3) ◽  
pp. 511-514 ◽  
Author(s):  
N. Appala Raju ◽  
J. Venkateswara Rao ◽  
K. Vanitha Prakash ◽  
K. Mukkanti
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Bromocresol Green ◽  
Bromophenol Blue ◽  
Green Method ◽  
Pharmaceutical Dosage Form ◽  
Extractable Complex ◽  
Bulk Drug ◽  
Tablet Dosage

Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of abacavir sulphate in its pharmaceutical dosage form. The method A and B are based on the formation of chloroform extractable complex of abacavir sulphate with bromophenol blue (method A) and bromocresol green (method B), which shows absorbance maxima at 460 nm and 469 nm respectively. The absorbance-concentration plot is linear over the range of 1-10 mcg/mL for method A and B respectively. Results of analysis for all the methods were validated statistically and by recovery studies. The proposed methods are economical and sensitive for the estimation of abacavir sulphate in bulk drug and in its tablet dosage form.

scholarly journals Spectrophotometric Determination of Zidovudine in Pharmaceutical Dosage Forms

2012 ◽  
Vol 9 (1) ◽  
pp. 89-92
Author(s):  
J. Sudhakar Reddy ◽  
MD. S. Maqsood Ahmed ◽  
I. E. Chakravarthy ◽  
K. Prabhavathi
Keyword(s):  
Statistical Analysis ◽  
Dosage Form ◽  
Dosage Forms ◽  
Ion Pair ◽  
Reagent Blank ◽  
Pharmaceutical Dosage Forms ◽  
Extractable Complex ◽  
Bulk Drug ◽  
Tablet Dosage

A simple, sensitive and economical spectrophotometric method has been developed for the determination of zidovudine in commercial dosage forms. The method was based on the formation of chloroform extractable complex of zidovudine with wool fast blue. The absorbance of the extractable ion pair complex is measured at the wavelength of maximum absorbance 590 nm against the reagent blank treated similarly. Statistical analysis proves that the proposed methods are reproducible and selective for the estimation of zidovudine in bulk drug and in its tablet dosage form.

scholarly journals Development and Validation of Reverse Phase HPLC Method for Simultaneous Estimation of Allopurinol and Lesinurad in its API and Pharmaceutical Dosage Form

2019 ◽  
Vol 4 (04) ◽  
pp. 50-57
Author(s):  
Sayma Khader ◽  
Ayesha Begum K ◽  
D. Ramakrishna
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Development And Validation ◽  
Tablet Dosage

A new, reliable, and validated reverse phase-high-performance liquid chromatography (HPLC) method was developed to quantify the amount of allopurinol and lesinurad simultaneously in solid (tablet) dosage form. A clear chromatographic division was attained on inertsil ODS (4.6 x 250 mm, 5 mm) column, and a mixture of 0.1% trifluoroacetic acid and methanol in the ratio of 40:60 v/v was used as mobile phase. The rate of flow was set at 1 mL/min, and UV detection was achieved at λmax of 255 nm. Injection volume was set to 20 μL. The correlation coefficient of 0.999 was established, and the accurateness was found to be 100.69 and 100.49 for both the drugs, respectively. Therefore, the developed method was simple, specific, precise, and stable. Hence, the method can be employed to estimate the said drugs in other pharmaceutical formulations.

scholarly journals Spectrophotometric Determination of Raloxifene Hydrochloride in Pharmaceutical Formulations

2007 ◽  
Vol 4 (1) ◽  
pp. 79-82 ◽  
Author(s):  
M. Mathrusri Annapurna ◽  
M. E. Bhanoji Rao ◽  
B. V. V. Ravi Kumar
Keyword(s):  
Ferric Chloride ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Reagent Blank ◽  
Spectrophotometric Methods ◽  
Maximum Absorbance ◽  
Bulk Drug ◽  
Raloxifene Hydrochloride ◽  
Tablet Dosage

Three new simple, sensitive, rapid and economical spectrophotometric Methods (A, B and C) have been developed for the determination of Raloxifene Hydrochloride in pharmaceutical bulk and tablet dosage form. Method A is based on the formation of yellow colored chromogen with 0.1N Sodium hydroxide exhibiting maximum absorbance against the corresponding reagent blank. The method B is based on the reaction of Raloxifene with Ferric chloride and 1, 10-phenanthroline to form blood red colored chromogen. The method C is based on the formation of blood red colored chromogen with Ferric chloride and 2, 2' bipyridyl. The absorbencies of the chromogens were measured at their respective wavelength of maximum absorbance against the corresponding reagent blank. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate.

scholarly journals Spectrophotometric Estimation of Amoxicillin Trihydrate in Bulk and Pharmaceutical Dosage Form

2008 ◽  
Vol 5 (s2) ◽  
pp. 1114-1116 ◽  
Author(s):  
K. Prakash ◽  
P. Narayana Raju ◽  
K. Shanta Kumari ◽  
M. Lakshmi Narasu
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Phosphate Buffer ◽  
Dosage Form ◽  
Molar Absorptivity ◽  
Pharmaceutical Dosage Form ◽  
Amoxicillin Trihydrate ◽  
Bulk Drug ◽  
Tablet Dosage

New simple, sensitive, rapid, reproducible and economical spectrophotometric method have been developed for the determination of amoxicillin trihydrate in pharmaceutical bulk and tablet dosage form using citro phosphate buffer pH 7.2. The system obeys Lambert-Beer's law at 231 nm in the concentration range 2.5-50 μg/m. Molar absorptivity, correlation coefficient and Sandell's sensitivity values were found to be 1.0020 x 104mol-1cm,-10.9996 and 0.03906 μg cm-2respectively. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate

scholarly journals A Novel Ultra Performance Liquid Chromatographic Method for the Estimation of Lercanidipine in Bulk and Tablet Dosage Form

2021 ◽  
pp. 768-776
Author(s):  
Satyabrata Sahu ◽  
U. Sunila Kumar Patra ◽  
Pratap Kumar Patra
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Ultra Performance Liquid Chromatography ◽  
Stability Study ◽  
Stability Indicating ◽  
Ich Guidelines ◽  
Liquid Chromatographic Method ◽  
Bulk Drug ◽  
Tablet Dosage ◽  
Liquid Chromatographic

Aim: In this present study an accurate reverse phase ultra-performance liquid chromatography (RP-UPLC) method has been developed, validated and applied to stability indicating studies to determine Lercanidipine HCL in bulk and marketed dosage form. Methods: Optimized chromatographic conditions were achieved by using Waters Acquity BEH C18 (2.1 x 50mm, 1.7m) UPLC column. Empower 2 is a software, dihydrogen Orthophosphate Buffer : Methanol (40 : 60)  as eluent at flow rate 0.3 ml/min. PDA detection was performed at 254nm. Results: The developed method was validated and stability study was conducted as per ICH guidelines. The retention time was found at 0.503 min. The method shows linearity over a range of 1 μg/ml to 60 μg /ml with the obtained correlation coefficient is 0.999. The LOD and LOQ values were found 0.025 and 0.05 μg /ml. The acidic and peroxide stressed study shows more degradation of 6.23% and 3.03%. Conclusion: The present developed method was found stability indicating, reliable, validated method was applied for the routine analysis of lercanidipine in bulk drug and the pharmaceutical formulations.

scholarly journals Development and Validation of a Stability-Indicating HPTLC Method for Analysis of Rasagiline Mesylate in the Bulk Drug and Tablet Dosage Form

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Singaram Kathirvel ◽  
Suggala Venkata Satyanarayana ◽  
Garikapati Devalarao
Keyword(s):  
Dosage Form ◽  
Degradation Products ◽  
Linear Regression Analysis ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Ich Guidelines ◽  
Bulk Drug ◽  
Rasagiline Mesylate ◽  
Development And Validation ◽  
Tablet Dosage

A simple and sensitive thin-layer chromatographic method has been established for analysis of rasagiline mesylate in pharmaceutical dosage form. Chromatography on silica gel 60 F254 plates with 6 : 1 : 2(v/v/v) butanol-methanol water as mobile phase furnished compact spots at Rf  0.76±0.01. Densitometric analysis was performed at 254 nm. To show the specificity of the method, rasagiline mesylate was subjected to acid, base, neutral hydrolysis, oxidation, photolysis, and thermal decomposition, and the peaks of degradation products were well resolved from that of the pure drug. Linear regression analysis revealed a good linear relationship between peak area and amount of rasagiline mesylate in the range of 100–350 ng/band. The minimum amount of rasagiline mesylate that could be authentically detected and quantified was 11.12 and 37.21 ng/band, respectively. The method was validated, in accordance with ICH guidelines for precision, accuracy, and robustness. Since the method could effectively separate the drug from its degradation products, it can be regarded as stability indicating.

STABILITY-INDICATING HPTLC METHOD FOR DETERMINATION OF DACLATASVIR IN PHARMACEUTICAL DOSAGE FORM

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (4) ◽  
pp. 56-62
Author(s):  
Hemlata M. Nimje ◽  
◽  
Meenakshi N. Deodhar
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Degradation Products ◽  
Assay Method ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Tlc Plates ◽  
Rf Values ◽  
Bulk Drug ◽  
Tablet Dosage

A simple, specific, precise, and accurate stability-indicating assay method using high performance thinlayer chromatography (HPTLC) is described for estimation of daclatasvir dihydrochloride (DCV) in bulk drug and in the tablet dosage form. The separations were achieved on prepared TLC plates precoated with silica gel 60 F254. The mobile phase developed and optimized for bringing out the separation involves toluene: methanol in the ratio of (6:4 V/V). The densitometric scanning wavelength selected was 312.0 nm. The compact bands of DCV were obtained at RF value of 0.702±0.032. The method developed was able to separate peaks of all the degradation products formed in ICH prescribed stress conditions with sufficient difference in their RF values. The developed method was validated for linearity and range, specificity, precision, accuracy, and robustness, and the results were found to be within acceptance criteria. The reliability of the method was evaluated when it was applied for the estimation of DCV in pharmaceutical tablet formulation and assay results gave good recovery.

scholarly journals Development and Validation of Direct Spectrophotometric Method for the Estimation of Glimepiride

2021 ◽  
pp. 176-186
Author(s):  
Dilip Chafle ◽  
Lata Awale
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Least Square ◽  
Research Centre ◽  
Bromophenol Blue ◽  
Blue Dye ◽  
Statistical Parameters ◽  
Pharmaceutical Dosage Form ◽  
Spectrophotometric Methods

Aims: To develop simple, sensitive and direct spectrophotometric methods for the estimation of widely prescribed antidiabetic hypoglycemic agent Glimepiride in pure and pharmaceutical dosage form. Place and duration of study: Research Centre of Taywade Group of Institution Koradi, Nagpur affiliated to Rashtrasant Tukadoji Maharaj Nagpur University Nagpur between June 2020 and March 2021. Methodology: Two spectrophotometric methods were developed based on ion-pair formation of drug with Cresol Red dye (Method A) and Bromophenol Blue dye (Method B) in methanol and chloroform. The possible reaction mechanism was proposed with evaluation of statistical parameters. The methods were validated for its application to determine Glimepiride in bulk as well as in pharmaceutical formulations Results: The Beer's law was found linear in the range 10 - 60 µgml-1 at 450 nm for method A and 2 - 20 µgml-1 at 578 nm for method B, respectively. The linear regression equation obtained by applying least square regression analysis for Glimepiride were found to be Absorbance = 0.0136 x Concentration in µgml-1 + 0.028; R2 = 0.9965 for method A and Absorbance = 0.0428x Concentration in µgml-1 +0.0722; R2 = 0.9949 for method B. The Sandell's sensitivity was found to be 0.0696 and 0.0177 for method A and B respectively. The apparent molar absorptivity calculated to be for both methods were 6.6724 x103 and 2.0999 x104 l mol-1 cm-1. Conclusion: Two direct spectrophotometric methods for determination of Glimepiride have been developed successfully and validated for sensitivity, accuracy, precision, repeatability and robustness as per ICH guidelines. The developed methods are suitable for the routine estimation of Glimepiride in pure and dosage form.

Novel UV Spectrophotometric Method for the Quantitative Analysis of CefpodoximeProxetil in Pharmaceutical Formulations by First Derivative Technique

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Potdar S. S. ◽  
Karajgi S. R. ◽  
Simpi C. C. ◽  
Kalyane N. V.
Keyword(s):  
Quantitative Analysis ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
First Derivative ◽  
Good Linearity ◽  
Beer's Law ◽  
Beer’S Law ◽  
Tablet Dosage ◽  
Ich Guideline

The spectrophotometric method for estimation of CefpodoximeProxetil employed first derivative amplitude UV spectrophotometric method for analysis using methanol as solvent for the drug. CefpodoximeProxetil has absorbance maxima at 235nm and obeys Beer’s law in concentration range 10-50µg/ml with good linearity i.e. r2 about 0.999. The recovery studies established accuracy of the proposed method; result validated according to ICH guideline. Results were found satisfactory and reproducible. The method was successfully for evaluation of CefpodoximeProxetil in tablet dosage form without interference of common excipients.

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