Airborne fungus at Pavia (Italy)

Entre el 1º de Febrero de 1979 al 28 de Febrero de 1980, fueron expuestas en Pavia, 3 veces por semana, tres placas de petri de 16 cm de diam. conteniendo PDA. Se aislaron un total de 12.734 colonias (pertenecientes a 46 géneros y 88 especies).Muchos de los aislamientos (54,6%), fueron especies de los géneros Cladosporium (13,4%), Epicoccum (11,7%), Aureobasidium (9,3%), Alternaría (7,9%), Penicillium (6,4%) y Botrytis (5,9%).Alternarla, Cladosporium y Epicoccum, aumentan en el Verano avanzado y en los inicios de Otoño. Aureobasidium, Aspergillus y Penicillium son frecuentes en invierno; Botrytis, es abundante en Primavera. Las especies dominantes aisladas fueron: Alternaria alternata, A. longipes, Aspergillus fumigatus, A.niger y A. flavus, Ameobasidium pullulans var. pullulans, Botrytis cinerea, Cladosporium cladosporioides, Epicoccum purpurascens, Penicillium janthinellum. Las especies de Fusarium roseum var. gibbosum (= F. equiseti), Phoma destructiva, Rhodotorula glutinis y Sporobolomyces roseus fueron también numerosas.

Yeasts producing zeatin

The present paper describes the first screening study of the ability of natural yeast strains to synthesize in culture the plant-related cytokine hormone zeatin, which was carried out using HPLC-MS/MS. A collection of 76 wild strains of 36 yeast species (23 genera) isolated from a variety of natural substrates was tested for the production of zeatin using HPLC-MS/MS. Zeatin was detected in more than a half (55%) of studied strains and was more frequently observed among basidiomycetous than ascomycetous species. The amount of zeatin accumulated during the experiment varied among species and strains. Highest zeatin values were recorded for basidiomyceteSporobolomyces roseusand ascomyceteTaphrinasp. that produced up to 8,850.0 ng and 5,166.4 ng of zeatin per g of dry biomass, respectively. On average, the ability to produce zeatin was more pronounced among species isolated from the arctic-alpine zone than among strains from tropical and temperate climates. Our study also demonstrated that epiphytic strains and pigmented yeast species, typically for phyllosphere, are able to more often produce a plant hormone zeatin than other yeasts.

Removal of Copper (II) Ions from Aqua Growth Medium by Red Yeast

Copper is a natural fungicide and is the active component of various pesticides. We detected uptake of higher concentration of the copper ions and responses to this stress in combination with presence hydrogen peroxide as a source of free radicals were studied on the three red yeast strains of species Rhodotorula glutinis (two strains) and Sporobolomyces roseus (one strain). The maximum Cu sorption was observed at the cells of strain Rhodotorula glutinis CCY 20-2-33 (25,18 mg/g dry weight) and at their exopolymers which accumulated the amount 10.22 mg/g dry weight. The remaining copper was sorbed onto the fibrillar part of cell wall (3.75 mg/g dry weight). The presence peroxide (oxidative stress) in cultivation medium decreased of the toleration of yeasts to Cu2+ ions and cells were able to take up less of about 17 % (from 3mM on 2,5 mM), although total uptake was lower about 11.01-15.96 %. We found that the strains of Rhodotorula glutinis are able to uptake about 44 % more copper ions (25.18-24.32 mg/g dry weight) in compared with strain of Sporobolomyces roseus (16.92 dry weight). However, the addition of peroxide into the cultivation medium the addition of affecting trade changes by reduce of the ability to uptake Cu2+ ions. The exopolymers and fibrillar part of cell wall these yeast were used as biopolymers with high sorption ability for metals.

Yeasts occurring on wheat seeds. III. A taxonomic study of strains of Sporobolomyces roseus Kluyver et van Niel and S. pararoseus Olson et Hammer

Morphology and physiology of strains of <i>Sporobolomvces roseus</i> and <i>S. pararoseus</i> isolated from winter wheat seeds was studied. Most of strains <i>S. roseus</i> and all strains of <i>S. pararoseus</i> exhibited a positive starch reaction, and showed a tendency to lose the ability of formation of ballistospores. In some strains of <i>S. pararoseus</i> adaptation to nilratc nitrogen occurred.

Granulomatous Meningoencephalitis Associated With Sporobolomyces roseus in a Dog

A German Shepherd Dog was evaluated for clinical signs of multifocal, progressive brain disease. Despite supportive care, the dog died shortly after hospital admission. Granulomatous meningoencephalitis with intralesional fungal hyphae was diagnosed postmortem. The fungus was identified as Sporobolomyces roseus by polymerase chain reaction amplification and sequencing of the ITS2 region.