scholarly journals Yeasts producing zeatin

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6474 ◽  
Author(s):  
Rostislav A. Streletskii ◽  
Aleksey V. Kachalkin ◽  
Anna M. Glushakova ◽  
Andrey M. Yurkov ◽  
Vladimir V. Demin
Keyword(s):  
Plant Hormone ◽  
Yeast Species ◽  
The Arctic ◽  
Alpine Zone ◽  
Yeast Strains ◽  
Dry Biomass ◽  
Screening Study ◽  
Sporobolomyces Roseus ◽  
Wild Strains ◽  
Temperate Climates

The present paper describes the first screening study of the ability of natural yeast strains to synthesize in culture the plant-related cytokine hormone zeatin, which was carried out using HPLC-MS/MS. A collection of 76 wild strains of 36 yeast species (23 genera) isolated from a variety of natural substrates was tested for the production of zeatin using HPLC-MS/MS. Zeatin was detected in more than a half (55%) of studied strains and was more frequently observed among basidiomycetous than ascomycetous species. The amount of zeatin accumulated during the experiment varied among species and strains. Highest zeatin values were recorded for basidiomyceteSporobolomyces roseusand ascomyceteTaphrinasp. that produced up to 8,850.0 ng and 5,166.4 ng of zeatin per g of dry biomass, respectively. On average, the ability to produce zeatin was more pronounced among species isolated from the arctic-alpine zone than among strains from tropical and temperate climates. Our study also demonstrated that epiphytic strains and pigmented yeast species, typically for phyllosphere, are able to more often produce a plant hormone zeatin than other yeasts.

scholarly journals Yeast Biodiversity in Fermented Doughs and Raw Cereal Matrices and the Study of Technological Traits of Selected Strains Isolated in Spain

2020 ◽  
Vol 9 (1) ◽  
pp. 47
Author(s):  
Rosana Chiva ◽  
Lorena Celador-Lera ◽  
José Antonio Uña ◽  
Ana Jiménez-López ◽  
María Espinosa-Alcantud ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Yeast Species ◽  
Dough Rheology ◽  
Phenotypic Traits ◽  
Fermentation Time ◽  
Yeast Strains ◽  
Wide Range ◽  
Wild Strains ◽  
Food Matrices ◽  
Comprehensive Study

Bakers use pure microorganisms and/or traditional sourdoughs as the leavening agent for making bread. The performance of each starter and the substances produced by the microorganisms greatly affect the dough rheology and features of breads. Modern sourdoughs inoculated with selected lactic acid bacteria and yeasts are microbiologically stable, safer than traditional sourdoughs, and easy to use. However, the commercial repertoire of baker’s yeasts is still limited. Therefore, there is a demand for new strains of yeast species, capable of conferring distinctive traits to breads made from a variety of agri-food matrices, in the design of innovative starters. In this context, we report the first comprehensive study on yeasts isolated from a wide range of fermented doughs, cereal flours, and grains of Spain. Nine yeast species were identified from 433 isolates, which were distributed among separate clades. Moreover, phenotypic traits of potential technological relevance were identified in selected yeast strains. Mother doughs (MDs) showed the greatest yeast biodiversity, whereas commercial Saccharomyces starters or related and wild strains often dominated the bakery doughs. A metataxonomic analysis of wheat and tritordeum MDs revealed a greater richness of yeast species and percentage variations related to the consistency, flour type, and fermentation time of MDs.

scholarly journals Contribution to the biogeography of arctic-alpine fungi: first records in the Southern Carpathians (Romania)

Sommerfeltia ◽  
2008 ◽  
Vol 31 (1) ◽  
pp. 191-211 ◽  
Author(s):  
A. Ronikier
Keyword(s):  
Geographical Distribution ◽  
The Arctic ◽  
Alpine Zone ◽  
Interesting Species ◽  
Southern Carpathians ◽  
Eleven Species

Contribution to the biogeography of arctic-alpine fungi: first records in the Southern Carpathians (Romania)A list of 24 species collected in the alpine zone of the Southern Carpathians is given. Fourteen species are new to Romania and the Southern Carpathians; five have not been previously reported from the Carpathian Mts. A synopsis of the geographical distribution of eleven species representing the arctic-alpine element is included. The seven most interesting species, Inocybe alboperonata, I. microfastigiata, I. nespiakii, I. oreina, Lactarius brunneoviolaceus, L. nanus, and Russula heterochroa, are described and illustrations of their microcharacters are given.

scholarly journals Candida cabralensis sp. nov., a yeast species isolated from traditional Spanish blue-veined Cabrales cheese

2010 ◽  
Vol 60 (11) ◽  
pp. 2671-2674 ◽  
Author(s):  
Ana Belén Flórez ◽  
Carmela Belloch ◽  
Pablo Álvarez-Martín ◽  
Amparo Querol ◽  
Baltasar Mayo
Keyword(s):  
Lactic Acid ◽  
Yeast Species ◽  
Rrna Gene ◽  
The Novel ◽  
Physiological Characterization ◽  
Yeast Strains ◽  
Unknown Species ◽  
Traditional Cheese ◽  
26S Rrna ◽  
Cabrales Cheese

Three yeast strains, 1AD8T, 3AD15 and 3AD23, belonging to a previously unknown yeast species were isolated from two independent batches of the Spanish blue-veined Cabrales cheese, a traditional cheese manufactured without the addition of starter and mould cultures. Physiological characterization revealed that the unknown yeast is not fermentative and does not assimilate lactose; rather it assimilates dl-lactic acid and ethanol, major end products of lactic acid bacteria metabolism in cheese. The novel yeast is anamorphic. Phylogenetic tree reconstruction based on nucleotide sequence comparison of the D1/D2 region of the 26S rRNA gene showed that Pichia terricola and Pichia fermentans are the closest relatives of the unknown species. The name Candida cabralensis sp. nov. is proposed, and the isolate 1AD8T (=CECT 13027T =CBS 11679T) is the type strain of this novel taxon.

Kloeckera taiwanica sp. nov., an ascomycetous apiculate yeast species isolated from mushroom fruiting bodies

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1434-1437 ◽  
Author(s):  
Chin-Feng Chang ◽  
Li-Yin Huang ◽  
Shan-Fu Chen ◽  
Ching-Fu Lee
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Distinct Species ◽  
Rrna Gene ◽  
Sporulation Medium ◽  
Yeast Strains ◽  
D2 Domain ◽  
Large Subunit Rrna

Three apiculate yeast strains, EJ7M09T, GJ5M15 and GJ15M04, isolated from mushrooms in Taiwan were found to represent a novel species of the genus Kloeckera. The phylogenetically closest relative of this novel species is Hanseniaspora occidentalis, but the type strain of H. occidentalis differed by 4.6 % divergence (25 substitutions; 5 gaps) in the sequence of the D1/D2 domain of the large subunit rRNA gene. This difference clearly suggests that the three strains represent a distinct species. As none of the strains that were examined in this study produced ascospores or exhibited conjugation on common sporulation medium either alone or in a pairwise mixture, this species could be considered as an anamorphic member of the genus Hanseniaspora, and a novel species, Kloeckera taiwanica sp. nov., is proposed, with EJ7M09T ( = BCRC 23182T = CBS 11434T) as the type strain.

CRABTREE POZİTİF VE CRABTREE NEGATİF MAYA TÜRLERİNDE GCR1 GENİNİN IN SILICO ANALİZİ

2021 ◽  
Vol 8 (17) ◽  
pp. 207-216
Author(s):  
Tülay TURGUT GENÇ ◽  
Ataberk ÇAKAN ◽  
Melih GÜNAY
Keyword(s):  
In Silico ◽  
Protein Production ◽  
Heterologous Protein ◽  
Yeast Species ◽  
Yeast Cells ◽  
Crabtree Effect ◽  
Promoter Regions ◽  
Yeast Strains ◽  
Genes Encoding ◽  
Chromatin Folding

The use of fermentation in the presence of oxygen and at high glucose concentrations is referred to as the Crabtree effect. Yeast species that have the Crabtree effect are called Crabtree positive, and yeast species that do not have the Crabtree effect are called Crabtree negative. While Crabtree negative yeast strains are mostly used for heterologous protein production in the industrial field, Crabtree positive yeast strains are used to understand metabolic events in cancer cells. The genes encoding the enzymes involved in the glycolytic pathway in S. cerevisiae yeast cells are controlled by Gcr1p. Gcr1p binds to CT elements located in the promoter regions of glycolytic genes and activates their transcription. In our study, Crabtree positive and negative yeast strains containing Sc-Gcr1p similar proteins were determined, and protein similarity analyzes and promoter analyzes of genes encoding the relevant proteins in these yeast strains were compared in silico using different databases and analysis programs. For this purpose, SGD, UNIPROT, NCBI-Genome and Yeastract databases and BLASTp-NCBI, MEGA-X and Chromatin Folding V2 programs were used. Using the SGD database, 32 different yeast strains were identified that matched with Sc-Gcr1p. Five different Crabtree positive and 5 different Crabtree negative yeast strains were selected from these yeast strains and in silico analyzes were performed using these yeast strains. After protein analysis and promoter analysis, it was determined that the similarities and differences between yeast species were not specific for Crabtree positive and Crabtree negative yeast species, but varied between species.

Cyberlindnera sylvatica sp. nov., a yeast species isolated from forest habitats

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Michael Brysch-Herzberg ◽  
Dénes Dlauchy ◽  
Martin Seidel ◽  
Gábor Péter
Keyword(s):  
Dna Sequences ◽  
Type Strain ◽  
Yeast Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
Sequence Comparisons ◽  
Yeast Strains ◽  
D2 Domain ◽  
Forest Habitats

Five yeast strains isolated from forest habitats in Hungary and Germany were characterized phenotypically and by sequencing of the D1/D2 domain of the large subunit rRNA gene and the ITS1-5.8S-ITS2 (ITS) region of the rRNA gene. The strains have identical D1/D2 domain and ITS region sequences. By sequence comparisons, Candida mycetangii and Candida maritima were identified as the closest relatives among the currently recognized yeast species. The DNA sequences of the investigated strains differ by 1.2 % (six substitutions) in the D1/D2 domain and by 3.5 % (12 substitutions and eight indels) in the ITS region from the type strain of C. mycetangii (CBS 8675T) while by 1.2 % (six substitutions and one indel) in the D1/D2 domain and by 7 % (32 substitutions and seven indels) in the ITS region from the type strain of C. maritima (CBS 5107T). Because the intraspecies heterogeneity seems to be very low and the distance to the most closely related species is above the commonly expected level for intraspecies variability Cyberlindnera sylvatica sp. nov. (holotype, CBS 16335T; isotype, NCAIM Y.02233T; MycoBank no., MB 835268) is proposed to accommodate the above-noted five yeast strains. Phenotypically the novel species can be distinguished from C. mycetangii and C. maritima by the formation of ascospores. Cyberlindnera sylvatica forms one or two hat-shaped ascospores per ascus on many different media as well as well-developed pseudohyphae and true hyphae. Additionally, we propose the transfer of three anamorphic members of the Cyberlindnera americana sub-clade to the genus Cyberlindnera as the following new taxonomic combinations Cyberlindnera maritima f.a., comb. nov., Cyberlindnera mycetangii f.a., comb. nov. and Cyberlindnera nakhonratchasimensis f.a., comb. nov.

scholarly journals Candida bombiphila sp. nov., a new asexual yeast species in the Wickerhamiella clade

2004 ◽  
Vol 54 (5) ◽  
pp. 1857-1859 ◽  
Author(s):  
Michael Brysch-Herzberg ◽  
Marc-André Lachance
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Yeast Strains ◽  
D2 Domain ◽  
Large Subunit Rdna

Two yeast strains were isolated from a bumblebee and bumblebee honey. The strains were almost identical in their D1/D2 domain of the large-subunit rDNA and their physiological abilities. In both respects the strains resembled Wickerhamiella domercqiae. On the basis of these data, it is proposed that the strains represent a novel species with the name Candida bombiphila sp. nov. The type strain is CBS 9712T (=NRRL Y-27640T=MH268T).

scholarly journals Inhibition of ochratoxin A production of Aspergillus carbonarius by yeast species

2011 ◽  
Vol 29 (No. 3) ◽  
pp. 291-297
Author(s):  
I. Var ◽  
Z. Erginkaya ◽  
B. Kabak
Keyword(s):  
Ochratoxin A ◽  
Yeast Species ◽  
Grape Juice ◽  
Rhodotorula Glutinis ◽  
Aspergillus Carbonarius ◽  
Yeast Strains ◽  
Candida Famata ◽  
Candida Lusitaniae ◽  
Yeast Extract Sucrose ◽  
Potential Use

A total of 21 yeast strains isolated from wine-grapes of Turkey were screened for their ability to inhibit ochratoxin A (OTA) accumulation by Aspergillus carbonarius in both yeast extract sucrose (YES) broth and grape juice. In monoculture, A. carbonarius produced a mean level of 39.03 µg/l and 21.32 µg/l OTA when grown in YES medium and grape juice, respectively. However, its ability to produce OTA in YES medium was greatly reduced in the presence of yeast strains except for Candida lusitaniae E2, Kloeckera spp. E4, and Rhodotorula glutinis D6. The percentage of inhibition of OTA production in YES medium ranged between 4.67% and 99.87%. Similarly, OTA production was inhibited in grape juice by more than 50% in the presence of yeast strains apart from Candida famata E6, R. glutinis, C. famata O3, Kloeckera spp. B3, and C. lusitaniae D9. The present study highlights the potential use of yeast isolates in the biocontrol of ochratoxin A-producing A. carbonarius.

Kodamaea transpacifica f.a., sp. nov., a yeast species isolated from ephemeral flowers and insects in the Galápagos Islands and Malaysia: further evidence for ancient human transpacific contacts

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4324-4329 ◽  
Author(s):  
Larissa F. D. Freitas ◽  
Enrique Javier Carvajal Barriga ◽  
Patricia Portero Barahona ◽  
Marc-André Lachance ◽  
Carlos A. Rosa
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Galápagos Islands ◽  
Galapagos Archipelago ◽  
Yeast Strains ◽  
Cameron Highlands ◽  
Ancient Times ◽  
Large Subunit Rrna

Twenty-four yeast strains were isolated from ephemeral flowers of Ipomoea spp. and Datura sp. and their associated insects in the Galápagos Archipelago, Ecuador, and from Ipomoea spp. and associated insects in the Cameron Highlands, Malaysia. Sequences of the D1/D2 domains of the large subunit rRNA gene indicated that these strains belong to a novel yeast species of the Kodamaea clade, although the formation of ascospores was not observed. The closest relative is Candida restingae. The human-mediated dispersion of this species by transpacific contacts in ancient times is suggested. The name Kodamaea transpacifica f.a., sp. nov. is proposed to accommodate these isolates. The type strain is CLQCA-24i-070T ( = CBS 12823T = NCYC 3852T); MycoBank number MB 803609.

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